12 results about "Indel polymorphism" patented technology

Provided in the present invention are the use of insertion / deletion polymorphism genetic markers in detecting the copy number of human chromosomal subtelomeric regions, a reagent panel for detecting the insertion / deletion polymorphisms genetic markers, and a method for detecting the copy number of human chromosomal subtelomeric regions.

The application of a molecular marker related to piglet growth traits relates to the field of livestock breeding, and aims to provide an InDel marker based on a pig HNF4A gene and application thereof in early selection of growth traits. By detecting an InDel marker in a first intron region of the pig HNF4A gene, the speed of pig breed selection is accelerated by using molecular marker assisted selection. The molecular marker corresponds to a 20-bp insertion / deletion polymorphism site between 46,821,377-46,821,378 bp of Chr17 in the international pig reference genome Sscrofa11.1 version sequence. The application can be used to accurately establish a pig population with high growth speed, thereby accelerating the selection and breeding process of pig growth traits.

The invention belongs to the technical field of gene detection, and particularly relates to a dual MIRA-LFD primer probe group, a kit and a method for identifying 23bp insertion / deletion polymorphic sites of a bovine prion protein gene promoter region. The primer probe group comprises an upstream primer as shown in SEQ ID NO: 2, a downstream primer as shown in SEQ ID NO: 5, an insertion type probe as shown in SEQ ID NO: 6 and a deletion type probe as shown in SEQ ID NO: 7. By utilizing the primer probe group disclosed by the invention, the 23bp insertion / deletion polymorphism of the bovine PRNP promoter region can be specifically detected, and a scientific basis is provided for bovine genotype identification and mad bovine disease susceptibility risk assessment.

The invention provides a primer group, a kit and a method for identifying 23bp insertion / deletion polymorphic sites of a bovine prion protein gene promoter region, and belongs to the technical field of gene detection. The primer group disclosed by the invention comprises an upstream outer primer 23-PF1, an upstream inner primer 23-IFA4, a downstream inner primer 23-AR2 and a downstream outer primer 23-PR3. A specific primer group is designed aiming at 23bp insertion / deletion polymorphic sites, an ARMS-PCR typing system is established by optimizing annealing temperature and primer concentration gradient, and 23bp insertion / deletion homozygotes and hybrid genotypes of the 23bp insertion / deletion homozygotes in a bovine prion protein gene promoter region can be specifically detected. The technical problem that the current identification of the 23bp insertion / deletion polymorphic site of the prion protein gene promoter region of the cattle product is tedious is solved, the disease resistance of the cattle to the mad cattle disease can be judged, and a scientific basis is provided for genotype identification of the cattle product and mad cattle disease susceptibility evaluation.

The present invention discloses a substance, method, and application for detecting multiple insertion / deletion polymorphisms in Leymus chinensis, belonging to the technical field of assays or detection methods involving enzymes, nucleic acids, or microorganisms. This application can be used to identify or assist in the identification of Leymus chinensis fruit set rate using 30 primer pairs, from InDel-1-P to InDel-30-P. These 30 primer pairs can accurately and efficiently identify varieties with this trait, facilitating the identification and screening of high-fruit-set Leymus chinensis germplasm during the seedling stage by breeders, accelerating the selection of new Leymus chinensis varieties.

This invention relates to the field of plant molecular breeding technology, specifically to an Indel marker EH-03-Indel-108 closely linked to ear height in maize and its application. This marker is located in a specific region of maize chromosome 3 and is a 4bp insertion / deletion polymorphic marker. Using specific primer pairs, PCR amplification, and agarose gel electrophoresis, ear haplotypes can be determined based on the 216bp band. It has wide applications in marker-assisted breeding of maize, enabling the screening of low ear height, lodging-resistant varieties. Compared with existing technologies, this invention can accurately identify the ear height trait in maize, improve the efficiency of lodging-resistant variety breeding, is simple to operate, low in cost, promotes maize genetic improvement, enhances the selectivity of breeding materials, and has significant theoretical and practical implications for the study of the molecular mechanisms of maize ear height and the process of genetic selection and improvement.

The application discloses application of an InDel molecular marker or a substance for detecting the InDel molecular marker in identification or auxiliary identification of pig growth rate, and the InDel molecular marker is a DNA molecule with a nucleotide sequence as shown in SEQ ID No. 1. The application has beneficial effects, including: (1) the application finds that an insertion / deletion site in a pig RPS27L gene promoter region can be used as a genetic selection marker for pig molecular breeding, and is beneficial to screening of pig breeds with excellent growth traits and acceleration of speed of selection and breeding of fine breeds; (2) the application uses a primer pair P1 designed according to a whole genome of a reference pig as a primer, uses pig genomic DNA as a template, and detects a genotype of an insertion / deletion polymorphism site (Chr1:108,880,114-108,880,126bp) in a pig RPS27L gene promoter region in a pig population through sequence amplification, electrophoretic identification and Sanger sequencing, and the detection is efficient, accurate and low in cost.

The present invention provides an InDel molecular marker for identifying resistance to Fusarium truncatum in corn species. The Fusarium truncatum resistance trait gene in corn species is located in the promoter region of the GRMZM2G083526 gene on corn chromosome 5, including two tightly linked InDel molecular markers, InDel-329 and InDel-395, respectively. The nucleotide sequence of InDel-329 is CATGTCC, and the nucleotide sequence of InDel-395 is CATCGAC, both of which show insertion / deletion polymorphisms. The present invention also provides an application of InDel molecular markers for use in a kit for detecting resistance to Fusarium truncatum in corn species, analysis of corn germplasm resources, or assisted genetic breeding. The present invention can effectively accelerate the corn seed breeding process.

The invention relates to the technical field of plant molecular breeding, in particular to an Indel marker EH-03-Internel-108 highly closely linked with a corn ear position and application of the Indel marker EH-03-Internel-108 highly closely linked with the corn ear position, and the marker is located in a specific region of a third chromosome of corn and is a 4bp insertion / deletion polymorphism marker. Through a specific primer pair, PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis, the ear position haplotype can be judged according to a 216bp band. The molecular marker is widely applied to corn molecular marker-assisted breeding, and low-ear lodging-resistant varieties can be screened. Compared with the prior art, the method has the advantages that the corn ear height character can be accurately identified, the lodging-resistant variety breeding efficiency is improved, the operation is simple and convenient, the cost is low, the corn genetic improvement is promoted, the breeding material selectivity is enhanced, and the method has important theoretical and practical significance on the molecular mechanism research and genetic breeding improvement process of the corn ear height character.

The present application relates to the technical field of plant molecular breeding, and particularly relates to an indel marker PH-03-Indel-106 related to the plant height trait of corn and application thereof. The indel marker is located in the region of the 3rd chromosome of corn Chr3:163962052-163964052, is a 7bp insertion / deletion polymorphism marker, the 7bp insertion sequence CTGAGGG exists in the tall inbred line of corn, and the insertion sequence does not exist in the short inbred line of corn. The specific primer pair PH-03-Indel-106F / R can specifically amplify the target region, and through 1.5% agarose gel electrophoresis detection, the 270bp band is determined as the short haplotype, and no band is the tall haplotype. The marker can be used for molecular marker assisted selection, breeding improvement and early identification and screening of the plant height trait of corn. The phenotype can be accurately predicted through DNA detection in the seedling stage, and environmental interference is avoided. The present application has the advantages of high specificity, simple detection, low cost, high throughput and the like, can significantly shorten the breeding period, realizes directional improvement of the plant height trait, and has important significance for genetic selection and plant type optimization of corn varieties.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhWRI1 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The molecular marker is located at InDel sites of 45bp, 716bp, 738bp-45bp, 718bp and 423bp of a No.8 chromosome of peanuts, the sequence of the molecular marker is SEQ ID NO.1 or SEQ ID NO.2, and a candidate gene of the molecular marker is named as AhWRI1. The KASP molecular marker is successfully developed on the basis of LTR reverse transcription transposon insertion / deletion polymorphism, the association between the KASP molecular marker and the oil content of the seed kernel is verified in a Yuhua 15 * Yueyan 20 recombinant inbred line population and a germplasm resource material, the accuracy of the marker is proved, and the KASP molecular marker can be applied to high-oil-content molecular marker-assisted selection breeding of the peanut seed kernel.

The invention provides a primer probe group, a kit and a method for identifying 12bp insertion / deletion polymorphic sites of an intron region of a bovine prion protein gene, and belongs to the technical field of gene detection. The primer probe group comprises 12 to mF1 as shown in SEQ ID NO: 2, 12 to mR2 as shown in SEQ ID NO: 4, 12 to IP1 as shown in SEQ ID NO: 6 and 12 to DP1 as shown in SEQ ID NO: 7, and a nucleotide sequence of an intron region 12bp is as shown in SEQ ID NO: 1. The primer probe group can specifically detect the 12bp insertion / deletion polymorphism of the bovine PRNP intron region, and provides a scientific basis for bovine genotype identification and mad bovine disease susceptibility risk assessment.