42 results about "Indel polymorphism" patented technology

The invention discloses a detection method for goat CSN1S1 gene insertion / deletion, and applications thereof. According to the method, the whole genomic DNA of a goat to be detected is used as a template, the goat CSN1S1 gene is amplified through a PCR technology, and agarose gel electrophoresis is performed to identify whether the indel polymorphism exists in the goat CSN1S1 gene. According to the present invention, the large sample (N is more than 1000) related analysis results reveal that the different types of the CSN1S1 gene indel are significantly related to the first lambing trait of Shanbei white cashmere goat, such that the different types of the CSN1S1 gene indel can be used as the DNA mark for improving the first lambing trait of the goat; and the method for rapidly detecting the goat CSN1S1 gene indel can be used in the marker-assisted selection breeding of goat reproductive traits in China, and is conducive to the rapid establishment of the goat genetic resource groups having the high lambing number trait.

The invention discloses a fast detection method for chicken Pax7 gene 31 bp indel polymorphism and the application thereof, wherein the detection method comprises the following steps: designing a pair of primers based on gene sequences of a third exon and a third intron of a chicken Pax7 gene; then performing PCR amplification; and finally detecting the 31 bp indel polymorphism of the gene through agarose gel electrophoresis, when the third intron 31 bp is inserted, the gene is an II genotype, when the third intron 31 bp is in deletion, the gene is a DD genotype, and when the locus is a heterozygous individual, the gene is an ID genotype. Meanwhile, the method utilizes detection result to perform relevance analyze with chicken economical characters, which is used for chicken assistant selection and molecular breeding. According to the invention, during big DNA fragment detection, the method not only has high resolution, sensitive detection and accurate type judgment, but also has the characteristics of simplicity in operation, time conservation and low cost, and can be widely popularized.

The invention discloses a detection method for 19-bp duplication and deletion polymorphism of a Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of the detection method. The method takes Chinese yellow cattle whole genome DNA (Deoxyribonucleic Acid) as a template, and a primer pair P1 is used for treating a partial segment of the Chinese yellow cattle PLAG1 gene through PCR (Polymerase Chain Reaction) amplification; then the segment obtained by the PCR amplification is subjected to agarose gel electrophoresis; a gene type of an insertion / deletion polymorphism siteon the Chinese yellow cattle PLAG1 gene is identified according to an electrophoresis result; different gene types of the duplication and deletion polymorphism of the gene are remarkably related withgrowth properties including body height, body length, chest circumference, chest width, chest depth, waist height and the like four types of Chinese yellow cattle including Qiachuan cattle, Nanyang cattle, Jiaxian cattle and Luxi cattle. The method disclosed by the invention can be applied to marker-assisted selection breeding of growth characters of beef cattle, and a good genetic resource groupwith good growth performance is easily and rapidly established.

The invention relates to a gallinaceous visfatin gene 9bp indel polymorphism detection method and application thereof. The detection method comprises the following steps of: designing a primer in the position of a gallinaceous visfatin gene 9bp indel locus; then carrying out PCR amplification; and detecting the polymorphism of the gene 9bp indel by the polyacrylamide gel electrophoresis, wherein the gene is of a DD genotype when 9bp is deleted, the gene is of an II genotype when 9bp is inserted, and the heterozygous individual at the locus is of an ID genotype. The correlation analysis combining the detection results and economic characters of chickens is used for the assistant selection and the molecular breeding of chickens. The detection method does not need be subject to the enzyme digestion, thereby saving the cost and the time; the detection is carried out by adopting the polyacrylamide gel electrophoresis, thereby overcoming the limitation of the agarose electrophoresis to the small-fragment nucleotide difference detection. The method has the advantages of high resolution, high detection sensitivity and accurate genotype judgment; and the gel has on requirements for the staining method, and the ethidium bromide (EB) staining and the silver staining are both applicable.

The invention discloses a fluorescent labeling complex amplification kit for insertion-deletion (InDel) polymorphism detection, and application of the kit. The kit comprises thirty-four InDel polymorphic sites and a sex locus (amelogenin). The fluorescent labeling complex amplification kit can be used for detecting the thirty-four InDel polymorphic sites and the one sex locus (amelogenin) at the same time; the kit can be used for detecting and genotyping 456 unrelated individuals from different ethnic groups from all over the country, has a cumulative personal identification rate reaching 0.99999985, and has a cumulative non-parent exclusion rate of 0.9989; the results prove that the kit is accurate in genotyping results, good in repeatability, high in sensitivity and accurate in genotyping results; therefore, a new method is provided for genetic relationship identification, sibling identification, individual identification, medical diagnosis, and the like.

The invention discloses a detection method of boar KDM5B gene insertion / deletion polymorphism and application. The detection method comprises the following steps: adopting a boar whole genome DNA as a template, amplifying KDM5B genes by virtue of PCR, then performing agarose gel electrophoresis, and identifying 35-bp insertion / deletion polymorphism of the KDM5B gene at a NC_010452.3: g.52599_52633 site. Different gene types of the 35-bp insertion / deletion polymorphism of the KDM5B gene are significantly related to reproductive traits such as short testicle axial length, heavy testicle and the like, the KDM5B gene 35-bp insertion / deletion polymorphism site can be used as a molecular marker for improving the reproductive traits of the Landrace boar, so that the marker assisted selection (MAS) of the Landrace boar reproductive traits is facilitated, and a boar population with excellent genetic resources can be rapidly established.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

The invention relates to a method for detecting insertion / deletion polymorphism of a sheep BMPR1B gene by using primer pairs. The detection method comprises the following steps: by taking sheep genomeDNA (deoxyribonucleic acid) to be detected as a template, and the primer pairs as amplification primers, performing PCR (polymerase chain reaction) amplification on a segment with insertion / deletionpolymorphism sites in a sheep BMPR1B gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion / deletion polymorphism sites according to electrophoresis results. By adopting the method provided by the invention, the primers are designed according to the insertion / deletion polymorphism sites (refer to a sequence NC_040257.1:g29373339_29373348) in the sheep BMPR1B gene intron region, the sheep genome DNA is adopted as the temperature, and the genotypes of the insertion / deletion polymorphism sites can be simply, rapidly and precisely detected with low cost through sequence amplification and electrophoresis identification.

The invention relates to a detection method for insertion / deletion polymorphism of a sheep RORA (retinoid acid receptor related orphan receptor A) gene. The detection method comprises the following steps: by taking genome DNA (deoxyribonucleic acid) of sheep to be detected as a template, and a primer pair as amplification primers, performing PCR (polymerase chain reaction) amplification on a fragment with insertion / deletion polymorphic sites in a sheep RORA gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion / deletion polymorphic sites according to electrophoresis results, wherein the insertion / deletion polymorphic sites are selected from 23-bp insertion / deletion polymorphic sites of NC_040258.1:S.10400_10423 sites of the sheep RORA gene. By adopting the method, the genotypes of the insertion / deletion polymorphic sites can be simply, rapidly and precisely detected with low cost.

The invention relates to a composite amplification system for jointly detecting InDel genetic markers of human full locus group, and a kit thereof. Particularly, the system jointly detects 45 Insertion / Deletion (InDel) polymorphic genetic markers on human autosomes and sex chromosomes. The composite amplification system comprises 45 pairs of primers marked by four fluorescent dyes (FAM, HEX, TAMRA and ROX) respectively, and can be used for composite amplification and detection of 45 InDel genetic markers in the same system. The kit can be used for detecting human biological samples and has the characteristics of high sensitivity, good repeatability, strong applicability and the like. 27 autosome InDel markers and 16 X chromosome InDel markers have high cumulative individual recognition rate and proper cumulative non-parent exclusion probability in unrelated individuals of Han nationality, and two Y chromosome InDel markers can meet the requirement of sex judgment in detection. Therefore, the detection kit can provide an effective supplementary detection means for forensic medicine practice.

The invention discloses a DNA detection method for detecting chest depth traits of Luxi blackhead rams and an application of the DNA detection method. The method comprises the following steps: by using whole genome DNA of the Luxi blackhead rams to be tested as a template and a primer pair P1 as amplification primers, performing amplification on a fragment containing an insertion / deletion polymorphic site of a fourth intron region of a sheep IGF2BP1 gene by using PCR, performing electrophoresis on PCR amplification products, and screening Luxi blackhead rams having excellent chest depth traitsaccording to the electrophoresis results. The method can use the IGF2BP1 gene to establish a kit for rapid identification of the Luxi blackhead ram genetic resource populations having the excellent chest depth traits, and can screen the sheep genetic resource populations having the excellent chest depth traits.

The invention relates to a primer pair for detecting insertion / deletion polymorphism of a sheep PRL gene. The primer pair can be used for PCR amplification of a fragment containing an insertion / deletion polymorphism site of an intron region of the sheep PRL gene. According to the primer pair, genotype of the insertion / deletion polymorphism site can be simply, quickly and accurately detected atlow cost. The detected insertion / deletion polymorphism site can be used as a molecular marker site for number of lambs produced by sheep, thereby accelerating an establishment of a sheep population with excellent lamb number traits and improving the breeding speed of improved varieties.

The invention relates to a molecular marker and a kit for predicting sudden cardiac death based on a polymorphic site of an METTL16 gene, the molecular marker is an rs58928048 site of the METTL16 gene, a base TGTCTG is inserted into or deleted from the site, and the insertion type indicates that the onset risk of sudden cardiac death is high. The invention provides a polymorphic molecular marker related to sudden cardiac death and further provides a sudden cardiac death detection kit by researching the correlation between insertion and deletion polymorphism of an rs58928048 site of an METTL16 gene and the phenotype of sudden cardiac death, so that the onset risk of sudden cardiac death of a detected individual is obtained.

The invention discloses a detection method of boar KDM5B gene insertion / deletion polymorphism and application. The detection method comprises the following steps: adopting a boar whole genome DNA as a template, amplifying KDM5B genes by virtue of PCR, then performing agarose gel electrophoresis, and identifying 35-bp insertion / deletion polymorphism of the KDM5B gene at a NC_010452.3: g.52599_52633 site. Different gene types of the 35-bp insertion / deletion polymorphism of the KDM5B gene are significantly related to reproductive traits such as short testicle axial length, heavy testicle and the like, the KDM5B gene 35-bp insertion / deletion polymorphism site can be used as a molecular marker for improving the reproductive traits of the Landrace boar, so that the marker assisted selection (MAS) of the Landrace boar reproductive traits is facilitated, and a boar population with excellent genetic resources can be rapidly established.

The invention relates to the technical field of biotechnology and livestock breeding, and particularly discloses a detection method for insertion / deletion polymorphism of a sheep CHCHD 7 gene and application thereof. The method comprises the following steps: taking a to-be-detected sheep whole genome DNA containing the CHCHD 7 gene as a template, performing PCR amplification on a fragment containing insertion / deletion polymorphic sites of a 3-flanking region of the sheep CHCHD 7 gene, performing electrophoresis on a PCR amplification product, and identifying the genotype of the insertion / deletion polymorphic sites according to an electrophoresis result. The insertion / deletion polymorphic sites detected by the method can be used as molecular markers of body length traits and chest depth traits of Tan sheep rams, so that rapid construction of Tan sheep populations with excellent three-dimensional scale traits is facilitated, and the breeding speed of improved varieties is increased.

The invention discloses a pig Oct4 gene insertion / lack detection method and application thereof. A boar genome DNA to be detected is used as a template; a primer pair P1 designed by referring to the pig genome is used as a primer; the Oct4 gene is amplified through a PCR (polymerase chain reaction) technology; then, agarose gel electrophoresis is performed. The insertion / lack polymorphism of the Oct4 gene in the NC_010449:g.2759-2760ins GGTTTTTGTCTA site is identified according to the electrophoresis result. The result shows that different types of the Oct4 gene 12-bp insertion / lack have the obvious relevance with the breeding properties of testicle weight, testicle long periphery length, testicle short periphery length and the like of 15-day-old large white boars; the result shows that different types of the Oct4 gene 12-bp insertion / lack can be used as DNA (deoxyribonucleic acid) marks of the boar breeding properties. The method is favorable for fast building the boar heredity resource groups with good breeding properties.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

The invention relates to a primer pair, kit and method for detecting sheep colony stimulating factor1 receptor (CSF1R) gene insertion / deletion polymorphism and an application of the method. The primerpair can be used for PCR amplification of a fragment containing sheep CSF1R gene intron region insertion / deletion polymorphic loci. The primers can be designed according to the sheep CSF1R gene intron region insertion / deletion polymorphic loci, genotypes of the insertion / deletion polymorphic loci can be detected simply, quickly, at low cost and accurately by using sheep genome DNA as a template through sequence amplification and electrophoretic identification, and different genotypes of the 27-bp insertion / deletion polymorphic loci have a significant correlation with mastitis traits of Australian white sheep. The method for detecting the sheep CSF1R gene insertion / deletion polymorphism provided by the invention can be applied to sheep molecular marker assisted selection breeding to accelerate the cultivation of sheep populations with high resistance to mastitis.

The invention discloses a DNA detection method for detecting the weight trait of black-head sheep in western Shandong and application of the DNA detection method. According to the method, a whole-genome DNA of a to-be-detected black-head sheep in western Shandong is used as a template, a primer pair P1 is used as an amplification primer, fragments inserted / deleted with polymorphic sites in the 5'UTR region of an HIAT1 gene of a sheep are amplified by utilizing PCR, the PCR amplified product is subjected to electrophoresis, and the black-head sheep in western Shandong with excellent weight trait is screened according to the electrophoresis result. The method can quickly identify the genetic resource population of black-head sheep in western Shandong with excellent weight trait and can quickly identify the sheep genetic resource population of sheep.

The invention relates to a primer pair for detecting sheep growth hormone receptor (GHR) gene insertion / deletion polymorphism. The primer pair can be used for PCR amplification of a fragment containing a sheep GHR gene intron region insertion / deletion polymorphic site. The method can detect the genotype of the insertion / deletion polymorphic site simply, quickly, at low cost and accurately, and thedetected insertion / deletion polymorphic site can be used as a molecular marker site for the number of lambs produced by sheep, so that the establishment of sheep populations with excellent litter size traits can be accelerated, and the speed of improved variety breeding can be improved.

The invention relates to a sheep KDM3B gene insertion / deletion polymorphism detection method, which comprises the following steps of adopting sheep genome DNA to be detected as a template, carrying out PCR amplification on an intron region insertion / deletion polymorphism site containing a sheep KDM3B gene, carrying out electrophoresis typing on the amplification product, and identifying the genotype corresponding to the insertion / deletion polymorphism site according to the electrophoresis result, wherein the insertion / deletion polymorphic site is selected from a 7-bp indel polymorphic site at the site of the sheep KDM3B gene NC_040256.1:g.52149274-52149280. According to the method, the genotype of the insertion / deletion polymorphic site can be simply, quickly and accurately detected with low cost, so that the Australian white sheep genetic resource population with excellent propagation traits can be quickly established, and the sheep population with excellent propagation genetic resources can be quickly established.

The invention belongs to the technical field of biomedicine, and particularly relates to application of polymorphism sites to preparation of products for detecting myeloproliterative neoplasms (MPN).According to the invention, through 269 patients clinically diagnosed as MPN in Qilu Hospital of Shandong University and 291 normal controls, statistical analysis is performed on the distribution situation of No. rs28362491 insertion / deletion polymorphism sites on an NF-kappa B gene in blood specimens, and data shows that the gene carrying type of the rs28364491 sites on the NF-kappa B gene is related to the susceptibility of the sites to MPN, thus providing a theoretical foundation for the occurrence mechanism of the myeloproliterative neoplasms, and providing a research foundation for development of a myeloproliterative neoplasms early warning model suitable for the population of China.

The invention discloses a detection method for insertion / deletion polymorphism of a sheep PDGFD gene, which comprises the following steps of: taking a to-be-detected sheep genome DNA (Deoxyribonucleic Acid) as a template, amplifying an insertion / deletion polymorphic locus in an intron region of the sheep PDGFD gene by using PCR (Polymerase Chain Reaction), performing electrophoresis typing on an amplified product, and identifying genotypes of the corresponding insertion / deletion polymorphic locus according to an electrophoresis result; and the insertion / deletion polymorphic locus is selected from the 18-bp indel polymorphic locus at the postition NC040266.1: g.3876993-3877010 of the sheep PDGFD gene. The method disclosed by the invention can be used for simply, conveniently and accurately detecting the genotype of the insertion / deletion polymorphic locus at low cost, so that a sheep genetic resource group with excellent characters can be quickly established.

The invention discloses a method for detecting insertion / deletion polymorphism of a sheep CRY2 gene. The method comprises the following steps: amplifying insertion / deletion polymorphism sites containing an intron region of the sheep CRY2 gene by using PCR by taking a to-be-detected sheep genome DNA as a template, carrying out electrophoretic typing on an amplification product, and identifying the genotype of the corresponding insertion / deletion polymorphism site according to the electrophoresis result, The insertion / deletion polymorphism sites are selected from a 6-bp indel polymorphic site at the NC_040266.1: g.82139190-82139195 site of the sheep CRY2 gene. According to the method disclosed by the invention, primers are designed according to the insertion / deletion polymorphism site of the intron region of the sheep CRY2 gene, and the genotype of the insertion / deletion polymorphism site can be simply, conveniently and accurately detected at low cost through sequence amplification and electrophoresis identification by taking sheep genome DNA as the template.

The invention discloses a detection method for goat CSN1S1 gene insertion / deletion, and applications thereof. According to the method, the whole genomic DNA of a goat to be detected is used as a template, the goat CSN1S1 gene is amplified through a PCR technology, and agarose gel electrophoresis is performed to identify whether the indel polymorphism exists in the goat CSN1S1 gene. According to the present invention, the large sample (N is more than 1000) related analysis results reveal that the different types of the CSN1S1 gene indel are significantly related to the first lambing trait of Shanbei white cashmere goat, such that the different types of the CSN1S1 gene indel can be used as the DNA mark for improving the first lambing trait of the goat; and the method for rapidly detecting the goat CSN1S1 gene indel can be used in the marker-assisted selection breeding of goat reproductive traits in China, and is conducive to the rapid establishment of the goat genetic resource groups having the high lambing number trait.

The invention relates to a chicken growth trait gene diagnosis kit and application thereof, belonging to the technical field of biological breeding. In the present invention, it is found that chicken LncFAM The 97bp indel polymorphic marker in the second intron region of the gene is related to chicken growth and development. For the 97bp homozygous insertion type II, homozygous deletion type DD or heterozygous ID, the II genotype individuals had the best growth status, followed by the ID genotype individuals, and the DD genotype individuals the worst. According to the indel polymorphic markers, corresponding chicken growth trait gene diagnostic primers and kits can be designed to detect 97bp homozygous inserted chicken individuals, and then quickly screen for fast-growing individuals for molecular breeding, thereby shortening the breeding time. Speed ​​up the breeding process.

The invention relates to a kit used for diagnosing / predicting sudden cardiac death (SCD). The kit comprises one or more of a specific primer pair (SEQ ID No. 1-SEQ ID No. 6) used for detecting the number rs3917 insertion / deletion polymorphic site on a COL1A2 gene, a specific primer pair (SEQ ID No. 7-SEQ ID No. 12) used for detecting the number rs72014506 insertion / deletion polymorphic site on anMIR155HG gene, a specific primer pair (SEQ ID No. 13-SEQ ID No. 18) used for detecting the number rs113044851 insertion / deletion polymorphic site on a CTH gene and a specific primer pair (SEQ ID No. 19-SEQ ID No. 24) used for detecting the number rs397729601 insertion / deletion polymorphic site on a DSG2 gene. The kit has the advantages that the SCD risks of a patient are evaluated through the insertion / deletion polymorphic evaluation, the associated analysis of the genotype of the four sites can favorably provide molecular-genetics support and help for the diagnosis and prediction of the SCD,and the kit has high application value in the risk evaluation and diagnosis of the SCD; in addition, the kit is good in detection specificity, high in sensitivity and good in accuracy.

The invention relates to a detection kit for a chicken CEL gene promoter 99bp indel polymorphic marker and application of the detection kit, and belongs to the technical field of molecular genetics breeding. According to the detection kit and the application, the CEL gene promoter 99bp indel polymorphic marker is found and can be used for assistant selection and molecular breeding of chickens; specifically, according to a sequence as shown in SEQ ID NO.1, a primer found to be without 183rd to 281st sites from the 5' end of the sequence is designed and amplified, whether the 183rd to 281st sites are lost or not is judged according to the size of the amplification product, then whether a detection sample is DD or II or ID gene type is judged, and the slaughter performance of local chickens is improved by enhancing breeding of DD gene type individuals. The application method established through the detection kit does not need digestion, is high in resolution, accurate in type judgment, easy to operate, low in cost and short in period, does not need special instruments, and is easy to popularize.