The invention relates to a detection kit for a chicken CEL gene promoter 99bp indel polymorphic marker and application of the detection kit, and belongs to the technical field of molecular genetics breeding. According to the detection kit and the application, the CEL gene promoter 99bp indel polymorphic marker is found and can be used for assistant selection and molecular breeding of chickens; specifically, according to a sequence as shown in SEQ ID NO.1, a primer found to be without 183rd to 281st sites from the 5' end of the sequence is designed and amplified, whether the 183rd to 281st sites are lost or not is judged according to the size of the amplification product, then whether a detection sample is DD or II or ID gene type is judged, and the slaughter performance of local chickens is improved by enhancing breeding of DD gene type individuals. The application method established through the detection kit does not need digestion, is high in resolution, accurate in type judgment, easy to operate, low in cost and short in period, does not need special instruments, and is easy to popularize.