5 results about "Indel polymorphism" patented technology

Provided in the present invention are the use of insertion / deletion polymorphism genetic markers in detecting the copy number of human chromosomal subtelomeric regions, a reagent panel for detecting the insertion / deletion polymorphisms genetic markers, and a method for detecting the copy number of human chromosomal subtelomeric regions.

This invention relates to the field of plant molecular breeding technology, specifically to an Indel marker EH-03-Indel-108 closely linked to ear height in maize and its application. This marker is located in a specific region of maize chromosome 3 and is a 4bp insertion / deletion polymorphic marker. Using specific primer pairs, PCR amplification, and agarose gel electrophoresis, ear haplotypes can be determined based on the 216bp band. It has wide applications in marker-assisted breeding of maize, enabling the screening of low ear height, lodging-resistant varieties. Compared with existing technologies, this invention can accurately identify the ear height trait in maize, improve the efficiency of lodging-resistant variety breeding, is simple to operate, low in cost, promotes maize genetic improvement, enhances the selectivity of breeding materials, and has significant theoretical and practical implications for the study of the molecular mechanisms of maize ear height and the process of genetic selection and improvement.

The application discloses application of an InDel molecular marker or a substance for detecting the InDel molecular marker in identification or auxiliary identification of pig growth rate, and the InDel molecular marker is a DNA molecule with a nucleotide sequence as shown in SEQ ID No. 1. The application has beneficial effects, including: (1) the application finds that an insertion / deletion site in a pig RPS27L gene promoter region can be used as a genetic selection marker for pig molecular breeding, and is beneficial to screening of pig breeds with excellent growth traits and acceleration of speed of selection and breeding of fine breeds; (2) the application uses a primer pair P1 designed according to a whole genome of a reference pig as a primer, uses pig genomic DNA as a template, and detects a genotype of an insertion / deletion polymorphism site (Chr1:108,880,114-108,880,126bp) in a pig RPS27L gene promoter region in a pig population through sequence amplification, electrophoretic identification and Sanger sequencing, and the detection is efficient, accurate and low in cost.

The present application relates to the technical field of plant molecular breeding, and particularly relates to an indel marker PH-03-Indel-106 related to the plant height trait of corn and application thereof. The indel marker is located in the region of the 3rd chromosome of corn Chr3:163962052-163964052, is a 7bp insertion / deletion polymorphism marker, the 7bp insertion sequence CTGAGGG exists in the tall inbred line of corn, and the insertion sequence does not exist in the short inbred line of corn. The specific primer pair PH-03-Indel-106F / R can specifically amplify the target region, and through 1.5% agarose gel electrophoresis detection, the 270bp band is determined as the short haplotype, and no band is the tall haplotype. The marker can be used for molecular marker assisted selection, breeding improvement and early identification and screening of the plant height trait of corn. The phenotype can be accurately predicted through DNA detection in the seedling stage, and environmental interference is avoided. The present application has the advantages of high specificity, simple detection, low cost, high throughput and the like, can significantly shorten the breeding period, realizes directional improvement of the plant height trait, and has important significance for genetic selection and plant type optimization of corn varieties.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhWRI1 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The molecular marker is located at InDel sites of 45bp, 716bp, 738bp-45bp, 718bp and 423bp of a No.8 chromosome of peanuts, the sequence of the molecular marker is SEQ ID NO.1 or SEQ ID NO.2, and a candidate gene of the molecular marker is named as AhWRI1. The KASP molecular marker is successfully developed on the basis of LTR reverse transcription transposon insertion / deletion polymorphism, the association between the KASP molecular marker and the oil content of the seed kernel is verified in a Yuhua 15 * Yueyan 20 recombinant inbred line population and a germplasm resource material, the accuracy of the marker is proved, and the KASP molecular marker can be applied to high-oil-content molecular marker-assisted selection breeding of the peanut seed kernel.