42 results about "Indel polymorphism" patented technology

The invention discloses a detection method of boar KDM5B gene insertion / deletion polymorphism and application. The detection method comprises the following steps: adopting a boar whole genome DNA as a template, amplifying KDM5B genes by virtue of PCR, then performing agarose gel electrophoresis, and identifying 35-bp insertion / deletion polymorphism of the KDM5B gene at a NC_010452.3: g.52599_52633 site. Different gene types of the 35-bp insertion / deletion polymorphism of the KDM5B gene are significantly related to reproductive traits such as short testicle axial length, heavy testicle and the like, the KDM5B gene 35-bp insertion / deletion polymorphism site can be used as a molecular marker for improving the reproductive traits of the Landrace boar, so that the marker assisted selection (MAS) of the Landrace boar reproductive traits is facilitated, and a boar population with excellent genetic resources can be rapidly established.

The invention discloses a DNA detection method for detecting breast depth traits of Luxi black ram and its application. The whole genome DNA of the Luxi black ram to be tested is used as a template, and the primer pair P1 is used as an amplification primer to amplify by PCR The fragment containing the insertion / deletion polymorphic site in the fourth intron region of the sheep IGF2BP1 gene was electrophoresed on the PCR amplification product, and the Luxi black ram with excellent breast depth was screened according to the electrophoresis results. This method can not only The IGF2BP1 gene was used to establish a population for rapid identification of Luxi black ram genetic resources with excellent chest depth traits, and at the same time to screen sheep genetic resource populations with excellent chest depth traits.

The invention discloses a pig Oct4 gene insertion / lack detection method and application thereof. A boar genome DNA to be detected is used as a template; a primer pair P1 designed by referring to the pig genome is used as a primer; the Oct4 gene is amplified through a PCR (polymerase chain reaction) technology; then, agarose gel electrophoresis is performed. The insertion / lack polymorphism of the Oct4 gene in the NC_010449:g.2759-2760ins GGTTTTTGTCTA site is identified according to the electrophoresis result. The result shows that different types of the Oct4 gene 12-bp insertion / lack have the obvious relevance with the breeding properties of testicle weight, testicle long periphery length, testicle short periphery length and the like of 15-day-old large white boars; the result shows that different types of the Oct4 gene 12-bp insertion / lack can be used as DNA (deoxyribonucleic acid) marks of the boar breeding properties. The method is favorable for fast building the boar heredity resource groups with good breeding properties.

The invention relates to a chicken growth trait gene diagnosis kit and application thereof, belonging to the technical field of biological breeding. In the present invention, it is found that chicken LncFAM The 97bp indel polymorphic marker in the second intron region of the gene is related to chicken growth and development. For the 97bp homozygous insertion type II, homozygous deletion type DD or heterozygous ID, the II genotype individuals had the best growth status, followed by the ID genotype individuals, and the DD genotype individuals the worst. According to the indel polymorphic markers, corresponding chicken growth trait gene diagnostic primers and kits can be designed to detect 97bp homozygous inserted chicken individuals, and then quickly screen for fast-growing individuals for molecular breeding, thereby shortening the breeding time. Speed ​​up the breeding process.

The invention discloses a fluorescent labeling compound amplification kit for insertion-deletion polymorphism detection and its application. The kit includes 34 InDel polymorphic sites and a sex locus (Amelogenin). The kit is a fluorescence-labeled multiple amplification kit that can detect 34 insertion-deletion polymorphic sites and a sex locus (Amelogenin) at the same time. Using the above-mentioned kit, 456 unrelated individuals from different ethnic groups across the country were detected and analyzed. Genotyping, the cumulative personal identification rate reached 0.99999985, and the cumulative non-parent exclusion rate was 0.9989. The results showed that the typing results of the kit were accurate, reproducible, high-sensitivity, and accurate. It can be used for genetic relationship identification, Sibling identification, individual identification and medical diagnosis provide a new method.