DNA detection method for detecting weight trait of black-head sheep in western Shandong and application of DNA detection method

A detection method and body weight technology, applied in the field of molecular biology, to achieve the effect of improving the speed of breeding of improved varieties and low cost

Active Publication Date: 2020-05-15
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on this gene is mainly focused on the field of structural biology and the research on the transport mechanism. There is no report on the significant correlation between the InDel site of the HIAT1 gene and the growth traits such as sheep weight.

Method used

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  • DNA detection method for detecting weight trait of black-head sheep in western Shandong and application of DNA detection method
  • DNA detection method for detecting weight trait of black-head sheep in western Shandong and application of DNA detection method
  • DNA detection method for detecting weight trait of black-head sheep in western Shandong and application of DNA detection method

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Experimental program
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Embodiment 1

[0025] The present invention utilizes the PCR method to detect the insertion / deletion polymorphism that may be produced by the mutation of the 5'UTR region of the sheep HIAT1 gene (reference sequence: NC_040252.1), and conducts correlation analysis with the weight traits of sheep to verify its Are there molecular markers that can be used as assisted selection in molecular breeding of sheep.

[0026] 1. Experimental drugs and reagents

[0027] 1.1 Biochemical reagents and biological reagents: ①Taq DNA polymerase (purchased from Fermantas or MBI Company); ②Proteinase K (purchased from Huamei Bioengineering Company); ③Marker I (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.).

[0028] 1.2 Common reagents: Tris, EDTA, NaCl, HCl, NaOH, Tris saturated phenol, chloroform, absolute ethanol, sodium dodecylsulfonate (SDS), ethidium bromide (EB), bromophenol blue, dimethyl Common reagents such as benzocyanide FF, boric acid, agarose, etc. were purchased from Huamei Bi...

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Abstract

The invention discloses a DNA detection method for detecting the weight trait of black-head sheep in western Shandong and application of the DNA detection method. According to the method, a whole-genome DNA of a to-be-detected black-head sheep in western Shandong is used as a template, a primer pair P1 is used as an amplification primer, fragments inserted/deleted with polymorphic sites in the 5'UTR region of an HIAT1 gene of a sheep are amplified by utilizing PCR, the PCR amplified product is subjected to electrophoresis, and the black-head sheep in western Shandong with excellent weight trait is screened according to the electrophoresis result. The method can quickly identify the genetic resource population of black-head sheep in western Shandong with excellent weight trait and can quickly identify the sheep genetic resource population of sheep.

Description

technical field [0001] The invention relates to a DNA detection method for detecting body weight traits of Luxi black-headed sheep and an application thereof, belonging to molecular biology. Background technique [0002] Therefore, mutton has attracted people's attention because of its delicious meat, high protein, low fat and other advantages. In the field of animal science research, in order to improve the meat yield of sheep, how to select sheep individuals with better growth traits has always been a key issue in animal genetics and breeding. [0003] Nowadays, molecular breeding methods will be combined with traditional breeding methods to jointly solve the genetic breeding problems of animals. Among them, the method of screening out an effective genetic marker to assist selective breeding through polymorphism research on related genes has gradually been widely recognized by people. [0004] Marker-assisted selection (Marker-assisted Selection, MAS) technology refers t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 宋恩亮雒云云姜富贵蓝贤勇成海建毛翠张果平魏晨
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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