A kind of chicken growth trait gene diagnosis kit and its application

A technology of growth status and genes, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problem of less research on Indel polymorphic markers

Active Publication Date: 2022-04-22
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on Indel polymorphic markers in long non-coding RNAs

Method used

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  • A kind of chicken growth trait gene diagnosis kit and its application
  • A kind of chicken growth trait gene diagnosis kit and its application
  • A kind of chicken growth trait gene diagnosis kit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In the present embodiment, the amplified fragment of the chicken growth trait gene diagnostic primer covers the insertion or deletion fragment region of 97bp in the second intron of the chicken LncFAM gene; specifically, the primer is designed according to the LncFAM gene sequence (GenBankAccession NC_006088) published by NCBI, The amplified fragment of the designed primer is the 461-557th position of the second intron region of the chicken LncFAM gene (the sequence of the second intron region of the LncFAM gene is shown in SEQ ID NO.1), and the primer sequences are as follows:

[0040] P-F: 5'-TCTCCACCCATTTTTATGCTGC-3' (as shown in SEQ ID NO.2);

[0041] P-R: 5'-TGTTGCCAGAAATATGCTTGCT-3' (shown in SEQ ID NO.3).

[0042] The size of the fragment amplified by the above primers is 303bp (as shown in SEQ ID NO.4) or 206bp (as shown in SEQ ID NO.5), 303bp is the I allele, and 206bp is the D allele. There is a 97bp insertion in the I allele, and the nucleotide sequence of t...

experiment example 1

[0072] In this experimental example, the correlation between the insertion or deletion of 97bp in the second intron of the chicken LncFAM gene and the growth status of the chicken was studied, the dominant genotypes were clarified, and individuals of these dominant genotypes were selected for breeding. The overall technical flow chart Such as figure 1 As shown, the primers used in the PCR reaction in the research are the primers in Example 1 of the chicken growth trait gene diagnostic primers, and the research process includes the following steps:

[0073] (1) Collection of samples

[0074] Collection of chicken farm F of Henan Agricultural University 2 Generation resource group (Gushi chicken × Anka) 800, 266 Xichuan silky chickens, 143 Lushi chickens, 129 Dongxiang chickens, 92 Changshun chickens, 143 Gushi chickens, Hailan brown-shell hens 222, 60 Roman brown-shell hens, 459 Hubbard broilers, 172 Ross 308 (308 refers to Ross 308 breed Albera chicken) and 300 AA broilers (...

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Abstract

The invention relates to a chicken growth trait gene diagnosis kit and application thereof, belonging to the technical field of biological breeding. In the present invention, it is found that chicken LncFAM The 97bp indel polymorphic marker in the second intron region of the gene is related to chicken growth and development. For the 97bp homozygous insertion type II, homozygous deletion type DD or heterozygous ID, the II genotype individuals had the best growth status, followed by the ID genotype individuals, and the DD genotype individuals the worst. According to the indel polymorphic markers, corresponding chicken growth trait gene diagnostic primers and kits can be designed to detect 97bp homozygous inserted chicken individuals, and then quickly screen for fast-growing individuals for molecular breeding, thereby shortening the breeding time. Speed ​​up the breeding process.

Description

technical field [0001] The invention relates to a chicken growth trait gene diagnosis kit and application thereof, belonging to the technical field of biological breeding. Background technique [0002] Genetic resources of livestock and poultry are important biological resources for protecting biodiversity, cultivating new varieties, and realizing sustainable development strategies of animal husbandry. There are abundant resources of local chicken breeds in our country, how to evaluate, protect and select them is an urgent problem to be solved. At present, with people's emphasis on the growth environment of livestock and poultry and the improvement of feed quality requirements, the production performance of livestock and poultry has been greatly improved. necessary material basis. However, unfavorable selection of poultry will lead to the loss of some dominant genotypes. Therefore, modern molecular markers can be used to improve the economic traits of local livestock and p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李转见经珍珠李文雅康相涛田亚东韩瑞丽孙桂荣王鑫磊王永才闫峰宾蒋瑞瑞李红李国喜刘小军李文婷陈悦
Owner HENAN AGRICULTURAL UNIVERSITY
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