Method and kit for detecting insertion/deletion polymorphism of sheep CRY2 gene, and application of kit

A technology for detection kits and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve problems such as patent publications that have not been found, and achieve improved breeding speed and low cost. cost effect

Inactive Publication Date: 2021-08-27
天津奥群牧业有限公司
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  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Method and kit for detecting insertion/deletion polymorphism of sheep CRY2 gene, and application of kit
  • Method and kit for detecting insertion/deletion polymorphism of sheep CRY2 gene, and application of kit
  • Method and kit for detecting insertion/deletion polymorphism of sheep CRY2 gene, and application of kit

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Embodiment Construction

[0030] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0031] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0032] A method for detecting insertion / deletion polymorphism of sheep CRY2 gene, said method comprising the following steps:

[0033] Using the sheep genomic DNA to be tested as a template, use PCR to amplify the insertion / deletion polymorphism site containing the intron region of the sheep CRY2 gene, and perform electrophoresis typing on the amplified product, and identify the corresponding insertion / deletion polymorphism a...

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Abstract

The invention discloses a method for detecting insertion / deletion polymorphism of a sheep CRY2 gene. The method comprises the following steps: amplifying insertion / deletion polymorphism sites containing an intron region of the sheep CRY2 gene by using PCR by taking a to-be-detected sheep genome DNA as a template, carrying out electrophoretic typing on an amplification product, and identifying the genotype of the corresponding insertion / deletion polymorphism site according to the electrophoresis result, The insertion / deletion polymorphism sites are selected from a 6-bp indel polymorphic site at the NC_040266.1: g.82139190-82139195 site of the sheep CRY2 gene. According to the method disclosed by the invention, primers are designed according to the insertion / deletion polymorphism site of the intron region of the sheep CRY2 gene, and the genotype of the insertion / deletion polymorphism site can be simply, conveniently and accurately detected at low cost through sequence amplification and electrophoresis identification by taking sheep genome DNA as the template.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a detection method, kit and application of sheep CRY2 gene insertion / deletion (indel) polymorphism. Background technique [0002] As one of the earliest animals domesticated by humans, sheep have important economic value in the animal husbandry industry and can provide people with a series of animal by-products such as mutton, goat milk, cashmere, and sheepskin. my country is the country with the largest amount of sheep breeding, slaughtering and mutton output. The reproductive traits of sheep directly affect the production efficiency of breeding enterprises. Modern sheep breeding aims to improve their production and reproductive performance, thereby greatly increasing their economic value. With the development of biological science and technology and the deepening of genetic research, the traditional selection method has gradually exposed the disadv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张清峰黄洋铭张东杰王春智苏鹏蓝贤勇潘传英王力林春建徐红伟
Owner 天津奥群牧业有限公司
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