A kind of detection method and application of pig oct4 gene insertion/deletion

Abstract

The invention discloses a pig Oct4 gene insertion / lack detection method and application thereof. A boar genome DNA to be detected is used as a template; a primer pair P1 designed by referring to the pig genome is used as a primer; the Oct4 gene is amplified through a PCR (polymerase chain reaction) technology; then, agarose gel electrophoresis is performed. The insertion / lack polymorphism of the Oct4 gene in the NC_010449:g.2759-2760ins GGTTTTTGTCTA site is identified according to the electrophoresis result. The result shows that different types of the Oct4 gene 12-bp insertion / lack have the obvious relevance with the breeding properties of testicle weight, testicle long periphery length, testicle short periphery length and the like of 15-day-old large white boars; the result shows that different types of the Oct4 gene 12-bp insertion / lack can be used as DNA (deoxyribonucleic acid) marks of the boar breeding properties. The method is favorable for fast building the boar heredity resource groups with good breeding properties.

Description

technical field [0001] The invention belongs to the field of modern biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion (indel), in particular to a method for detecting the 12-bp insertion / deletion (indel) of Oct4 gene in boars. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology first detects DNA polymorphisms of important genes, then analyzes the correlation between DNA polymorphisms and genetic traits, and finally Trait selection is then carried out based on DNA markers significantly associated with genetic traits. As a new technology born during the development of modern molecular biology technology, MAS breeding technology can quickly and accurately analyze th...

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Problems solved by technology

(2) Inversion of DNA fragments, functional gene inversion to an inactive heterochromosomal region, resulting in no or very little expression of the gene, resulting in phenotypic changes

There is a lack of research on the genetic variation of the pig Oct4 gene, and the functional research on this gene locus is even more blank

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