Hormone-free culture medium for culturing high-quality butterfly orchid seedlings and application of hormone-free culture medium
A culture medium, Phalaenopsis technology, applied in application, plant regeneration, horticulture, etc., can solve the problems of increasing somatic cell variation, prolonging the reproduction cycle, etc., reducing the variation rate, speeding up the cultivation speed, and convenient application Effect
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[0016] First, prepare the hormone-free series medium for the Phalaenopsis seedlings (including A, B, and C three mediums). The preparation of this series of medium adopts the conventional method of tissue culture medium preparation.
[0017] Medium A for aseptic seeding, containing the following components: Huaduoduo No. 11, 2.2g / L; Tryptone 2.5g / L; Activated carbon 2.0g / L; Glutathione 30mg / L; Sucrose 20g / L ; Agar powder 9.0g / L; the final pH is adjusted to 5.2 ~ 5.5.
[0018] Medium B for the cultivation of protocorm differentiation seedlings, which contains the following components: Huaduoduo No. 11, 2.2g / L; Tryptone 2.0g / L; Activated carbon 1.5g / L; Malic acid 30mg / L; Adenine 10mg / L; banana puree 50g / L; sucrose 15g / L; agar powder 8.0g / L; the final pH is adjusted to 5.0-5.5.
[0019] Medium C for the rooting culture of strong seedlings of Phalaenopsis seedlings. It contains the following components: Huaduoduo No. 11, 2.4g / L; Tryptone 2.5g / L; Activated carbon 1.0g / L; Malic acid 30mg...
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