Hormone-free culture medium for culturing high-quality butterfly orchid seedlings and application of hormone-free culture medium

Abstract

The invention discloses a hormone-free culture medium for culturing high-quality butterfly orchid seedlings. The hormone-free culture medium comprises three culture mediums A, B and C, wherein the culture medium A consists of 1.5-2.8g / L of Huaduoduo #11, 1.5-2.5g / L of tryptone, 1.0-3.0g / L of active carbon, 20-30mg / L of glutathione, 20-30g / L of saccharose and 8.0-9.0g / L of agar powder, and the final pH of the culture medium A is adjusted to 5.2-5.5. The invention also discloses application of three culture mediums A, B and C, of the hormone-free culture medium, and used in culture of the high-quality butterfly orchid seedlings. The series of the culture mediums and a method are simple and adaptive. The conveniently applied culture is suitable for germfree sowing of selfed or interbreeded seeds of most garden seeds in conventional butterfly orchid species, so that a large quantity of high-quality seedlings of the different species of high-quality butterfly orchids can be obtained; furthermore, the aberration rate of the seedlings is reduced as no hormone is added, and the culture speed of the high-quality butterfly orchid seedlings is increased.

Description

technical field

[0001] The invention belongs to cultivation of agricultural and forestry flowers, in particular to a hormone-free medium for cultivating high-quality Phalaenopsis seedlings and its application. Background technique

[0002] Phalaenopsis is a plant of the Orchidaceae Phalaenopsis genus. Its flower shape is strange and delicate, and the flowers are large and gorgeous. It has very high ornamental value and economic value. With the continuous improvement of people's living standards, the market demand for Phalaenopsis is also increasing. However, Phalaenopsis is a typical single-stem tropical epiphytic orchid. Side branches rarely occur in plants, and the ramet reproduction coefficient is extremely low. Phalaenopsis needs to be artificially pollinated to obtain seeds, but its seeds are underdeveloped, have no endosperm, and only have a very thin seed coat. It is difficult to germinate under natural conditions, and it needs to be aseptically sown by tissue culture...

Examples

Embodiment Construction

[0016] First prepare the hormone-free series culture medium (comprising A, B, C three kinds of culture medium) of Phalaenopsis seedling, the making of this series culture medium adopts the culture medium preparation method of tissue culture routine.

[0017] Medium A for aseptic sowing, containing the following components: Huaduoduo No. 11, 2.2g / L; tryptone 2.5g / L; activated carbon 2.0g / L; glutathione 30mg / L; sucrose 20g / L ; Agar powder 9.0g / L; The final pH is adjusted to 5.2-5.5.

[0018] Medium B for the cultivation of protocorm-differentiated seedlings, which contains the following components: Huaduoduo No. 11, 2.2g / L; tryptone 2.0g / L; activated carbon 1.5g / L; malic acid 30mg / L; adenine 10mg / L; banana puree 50g / L; sucrose 15g / L; agar powder 8.0g / L; final pH adjusted to 5.0-5.5.

[0019] Medium C for strong seedling rooting culture of Phalaenopsis seedlings, which contains the following components: Huaduoduo 11, 2.4g / L; tryptone 2.5g / L; activated carbon 1.0g / L; malic acid ...

technical field

[0001] The invention belongs to cultivation of agricultural and forestry flowers, in particular to a hormone-free medium for cultivating high-quality Phalaenopsis seedlings and its application. Background technique

[0002] Phalaenopsis is a plant of the Orchidaceae Phalaenopsis genus. Its flower shape is strange and delicate, and the flowers are large and gorgeous. It has very high ornamental value and economic value. With the continuous improvement of people's living standards, the market demand for Phalaenopsis is also increasing. However, Phalaenopsis is a typical single-stem tropical epiphytic orchid. Side branches rarely occur in plants, and the ramet reproduction coefficient is extremely low. Phalaenopsis needs to be artificially pollinated to obtain seeds, but its seeds are underdeveloped, have no endosperm, and only have a very thin seed coat. It is difficult to germinate under natural conditions, and it needs to be aseptically sown by tissue culture...

Examples

Embodiment Construction

[0016] First prepare the hormone-free series culture medium (comprising A, B, C three kinds of culture medium) of Phalaenopsis seedling, the making of this series culture medium adopts the culture medium preparation method of tissue culture routine.

[0017] Medium A for aseptic sowing, containing the following components: Huaduoduo No. 11, 2.2g / L; tryptone 2.5g / L; activated carbon 2.0g / L; glutathione 30mg / L; sucrose 20g / L ; Agar powder 9.0g / L; The final pH is adjusted to 5.2-5.5.

[0018] Medium B for the cultivation of protocorm-differentiated seedlings, which contains the following components: Huaduoduo No. 11, 2.2g / L; tryptone 2.0g / L; activated carbon 1.5g / L; malic acid 30mg / L; adenine 10mg / L; banana puree 50g / L; sucrose 15g / L; agar powder 8.0g / L; final pH adjusted to 5.0-5.5.

[0019] Medium C for strong seedling rooting culture of Phalaenopsis seedlings, which contains the following components: Huaduoduo 11, 2.4g / L; tryptone 2.5g / L; activated carbon 1.0g / L; malic acid ...