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Detection method for insertion/deletion polymorphism of sheep PDGFD gene, kit and application

A detection kit and detection method technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of undiscovered patent publications, etc., and achieve the effect of improving the breeding speed and low cost of fine varieties

Inactive Publication Date: 2021-08-20
天津奥群牧业有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Detection method for insertion/deletion polymorphism of sheep PDGFD gene, kit and application
  • Detection method for insertion/deletion polymorphism of sheep PDGFD gene, kit and application
  • Detection method for insertion/deletion polymorphism of sheep PDGFD gene, kit and application

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Embodiment Construction

[0029] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0030] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0031] A detection method for insertion / deletion polymorphism of sheep PDGFD gene, comprising the following steps:

[0032]Using the sheep genomic DNA to be tested as a template, use PCR to amplify the insertion / deletion polymorphism site containing the intron region of the sheep PDGFD gene, and perform electrophoresis typing on the amplified product, and identify the corresponding insertion / deletion polymorphism according to...

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Abstract

The invention discloses a detection method for insertion / deletion polymorphism of a sheep PDGFD gene, which comprises the following steps of: taking a to-be-detected sheep genome DNA (Deoxyribonucleic Acid) as a template, amplifying an insertion / deletion polymorphic locus in an intron region of the sheep PDGFD gene by using PCR (Polymerase Chain Reaction), performing electrophoresis typing on an amplified product, and identifying genotypes of the corresponding insertion / deletion polymorphic locus according to an electrophoresis result; and the insertion / deletion polymorphic locus is selected from the 18-bp indel polymorphic locus at the postition NC040266.1: g.3876993-3877010 of the sheep PDGFD gene. The method disclosed by the invention can be used for simply, conveniently and accurately detecting the genotype of the insertion / deletion polymorphic locus at low cost, so that a sheep genetic resource group with excellent characters can be quickly established.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a detection method, kit and application of sheep PDGFD gene insertion / deletion (indel) polymorphism. Background technique [0002] Sheep are an important part of my country's mutton sheep industry and play an important role in the structure of animal husbandry. At present, my country ranks first in the world in terms of sheep stock and annual slaughter, and is a veritable mutton producer. At the same time, my country is also a big mutton consumer. As an important economic trait of sheep, reproductive traits can affect the lambing rate of sheep, thus directly affecting the economic benefits of sheep industry. Modern sheep breeding aims to improve its production and reproductive performance thereby greatly increasing its economic value. In recent years, my country's mutton sheep production cannot meet people's growing consumption demand. To this end,...

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张清峰苏鹏蓝贤勇潘传英黄洋铭雒云云林春建
Owner 天津奥群牧业有限公司
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