Detection method for insertion/deletion polymorphism of sheep PDGFD gene, kit and application

A detection kit and detection method technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of undiscovered patent publications, etc., and achieve the effect of improving the breeding speed and low cost of fine varieties
CN113278708AInactive Publication Date: 2021-08-20天津奥群牧业有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
天津奥群牧业有限公司
Publication Date
2021-08-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection method for insertion / deletion polymorphism of a sheep PDGFD gene, which comprises the following steps of: taking a to-be-detected sheep genome DNA (Deoxyribonucleic Acid) as a template, amplifying an insertion / deletion polymorphic locus in an intron region of the sheep PDGFD gene by using PCR (Polymerase Chain Reaction), performing electrophoresis typing on an amplified product, and identifying genotypes of the corresponding insertion / deletion polymorphic locus according to an electrophoresis result; and the insertion / deletion polymorphic locus is selected from the 18-bp indel polymorphic locus at the postition NC040266.1: g.3876993-3877010 of the sheep PDGFD gene. The method disclosed by the invention can be used for simply, conveniently and accurately detecting the genotype of the insertion / deletion polymorphic locus at low cost, so that a sheep genetic resource group with excellent characters can be quickly established.
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Description

technical field

[0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a detection method, kit and application of sheep PDGFD gene insertion / deletion (indel) polymorphism. Background technique

[0002] Sheep are an important part of my country's mutton sheep industry and play an important role in the structure of animal husbandry. At present, my country ranks first in the world in terms of sheep stock and annual slaughter, and is a veritable mutton producer. At the same time, my country is also a big mutton consumer. As an important economic trait of sheep, reproductive traits can affect the lambing rate of sheep, thus directly affecting the economic benefits of sheep industry. Modern sheep breeding aims to improve its production and reproductive performance thereby greatly increasing its economic value. In recent years, my country's mutton sheep production cannot meet people's growing consumption demand. To this end,...

Claims

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