Detection method for insertion/ deletion polymorphism of sheep CHCHD 7 gene and application thereof

Abstract

The invention relates to the technical field of biotechnology and livestock breeding, and particularly discloses a detection method for insertion / deletion polymorphism of a sheep CHCHD 7 gene and application thereof. The method comprises the following steps: taking a to-be-detected sheep whole genome DNA containing the CHCHD 7 gene as a template, performing PCR amplification on a fragment containing insertion / deletion polymorphic sites of a 3-flanking region of the sheep CHCHD 7 gene, performing electrophoresis on a PCR amplification product, and identifying the genotype of the insertion / deletion polymorphic sites according to an electrophoresis result. The insertion / deletion polymorphic sites detected by the method can be used as molecular markers of body length traits and chest depth traits of Tan sheep rams, so that rapid construction of Tan sheep populations with excellent three-dimensional scale traits is facilitated, and the breeding speed of improved varieties is increased.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and livestock breeding, in particular to a rapid and accurate typing detection of the 8-bp insertion / deletion polymorphism (indel) site of sheep CHCHD7 gene NC_040260.1:g36219994_3622000 and its molecular markers Applications in assisted selection breeding. Background technique [0002] With the development of molecular genetics, people pay more and more attention to the study of the relationship between genes at the DNA molecular level, and dozens of major genes closely related to the inheritance of quantitative traits have been discovered so far. Marker-assisted selection (MAS) uses molecular markers related to quantitative traits, using marker information as auxiliary information, to accurately and quickly analyze the genetic composition of individuals from the molecular level, so as to achieve genotype selection. Since the introduction of MAS in 1990, more than 20 genetic markers have ...

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Problems solved by technology

Moreover, the soluble protein encoded by CHCHD7 is ubiquitously expressed in mammals, with the highest expression levels in adult tissues, especially heart, skeletal muscle, and pancreas; but lower expression levels in fetal tissues (Aspet al. , 2006)

However, there are no reports about the indel locus of CHCHD7 gene and its significant correlation with growth and development traits.

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