Molecular marker and kit for predicting sudden cardiac death based on METTL16 gene polymorphic site

A technology for sudden cardiac death and molecular markers, which can be used in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve problems such as predicting SCD susceptibility

Pending Publication Date: 2022-07-12
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the prior art, there is no research report on the correlation between the rs58928048 indel polymorphism and SCD, and there

Method used

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  • Molecular marker and kit for predicting sudden cardiac death based on METTL16 gene polymorphic site
  • Molecular marker and kit for predicting sudden cardiac death based on METTL16 gene polymorphic site

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The use of embodiment 1 detection kit

[0036] Step 1: Extraction of DNA Template

[0037] Genomic DNA from peripheral blood was extracted using a blood genomic DNA extraction system (non-spin column type).

[0038] Step 2: PCR reaction - replication of the target fragment

[0039] Use a PCR detection kit for SCD susceptibility, which contains the following primers:

[0040] SEQ ID NO.1: 5'-CACATGAATGCTTGGACTAGAAT-3', Tm value is 57°C;

[0041] SEQ ID NO.2: 5'-GTGTATTTTTTTTCCTGGAGAGA-3', the Tm value is 59°C;

[0042] The primer pair can specifically amplify the fragment containing the indel polymorphism of rs58928048 in the METTL16 gene.

[0043] The total volume of the PCR reaction system is 10uL, including: 1μL DNA template, 0.04μL each of 50μM specific primer pairs, 0.08μL of 2.5U / μL Taq DNA polymerase; 0.2μL of 2.5mM dNTP mixture; 25mM MgCl 2 Solution 0.6 μL; 10× PCR reaction buffer 1 μL; deionized water to make up;

[0044] The reaction was carried out on an...

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Abstract

The invention relates to a molecular marker and a kit for predicting sudden cardiac death based on a polymorphic site of an METTL16 gene, the molecular marker is an rs58928048 site of the METTL16 gene, a base TGTCTG is inserted into or deleted from the site, and the insertion type indicates that the onset risk of sudden cardiac death is high. The invention provides a polymorphic molecular marker related to sudden cardiac death and further provides a sudden cardiac death detection kit by researching the correlation between insertion and deletion polymorphism of an rs58928048 site of an METTL16 gene and the phenotype of sudden cardiac death, so that the onset risk of sudden cardiac death of a detected individual is obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker and a kit for predicting sudden cardiac death based on METTL16 gene polymorphism sites. Background technique [0002] Sudden cardiac death (SCD) refers to sudden and unexpected death caused by cardiac causes, which is manifested as sudden loss of consciousness and circulatory and respiratory arrest in a short period of time. "Short-term" is defined as less than 1 hour with witnesses and 24 hours without witnesses. A large number of previous studies have shown that the cause of death in SCD is mostly coronary atherosclerotic heart disease or fatal arrhythmia. The former is more common in elderly people, while the latter is more common in young people. Considering that it is difficult to find the exact cause of death in postmortem autopsy and histological examination, in order to accurately diagnose SCD, it is imminent to uncover the molecular mechanism of SCD and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 甄晓媛赵文锋李立娟高玉振
Owner SUZHOU UNIV
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