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Kit used for diagnosing/predicting sudden cardiac death

A technology for sudden cardiac death and kits, which is applied in the determination/examination of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of not predicting the susceptibility of SCD and diagnosing SCD.

Active Publication Date: 2018-03-30
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is no research report on the correlation between the insertion / deletion polymorphisms of the above four genes and SCD, and there is no joint detection of the insertion / deletion polymorphisms on the four genes to predict the susceptibility and Reports on the diagnosis of SCD

Method used

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  • Kit used for diagnosing/predicting sudden cardiac death
  • Kit used for diagnosing/predicting sudden cardiac death
  • Kit used for diagnosing/predicting sudden cardiac death

Examples

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Embodiment 1

[0041] A kit for diagnosing / predicting sudden cardiac death of the present invention, comprising a pair of specific primers labeled with fluorescent dyes for detecting the insertion / deletion polymorphism site rs3917 on the COL1A2 gene, rs72014506 on the MIR155HG gene The specific primer pair for the insertion / deletion polymorphic site, the specific primer pair for the insertion / deletion polymorphic site rs113044851 on the CTH gene, and the specificity for the insertion / deletion polymorphic site rs397729601 on the DSG2 gene primer pair. in

[0042] The specific primer pair for the rs3917 insertion / deletion polymorphic site on the COL1A2 gene uses the sequence shown in SEQ ID No.1 as a sense primer, and uses the sequence shown in SEQ ID No.2 as an antisense primer;

[0043] The specific primer pair for the rs72014506 insertion / deletion polymorphic site on the MIR155HG gene uses the sequence shown in SEQ ID No.7 as a sense primer, and uses the sequence shown in SEQ ID No.8 as an...

Embodiment 2

[0069] The kit for diagnosing / predicting sudden cardiac death in Example 1 of the present invention is used in the same manner as in Example 1, and its diagnosis of SCD is as follows:

[0070] The gene sequencing diagrams and SDS-PAGE gel electrophoresis diagrams of COLIA2 gene, MIR155HG gene, CTH gene and DSG2 gene are as follows Figure 1-4 shown. In the figure, A is an example of the sequencing results of the template strand, and the underline corresponds to the indel of the coding strand at the polymorphic site; B is from different individuals (numbered 1-14, where numbers 1, 2, 3, 8, 9, and 10 No. 1 sample is diagnosed as sudden cardiac death by forensic pathology anatomy, and the remaining samples are normal control group) DNA samples using the PCR amplification system of the present invention to obtain a schematic diagram of product electrophoresis.

[0071] figure 1 It shows that numbers 1 and 8 are deletion homozygotes, numbers 2, 4, 5, 6, 7, 11, 12, 13 and 14 are ins...

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Abstract

The invention relates to a kit used for diagnosing / predicting sudden cardiac death (SCD). The kit comprises one or more of a specific primer pair (SEQ ID No. 1-SEQ ID No. 6) used for detecting the number rs3917 insertion / deletion polymorphic site on a COL1A2 gene, a specific primer pair (SEQ ID No. 7-SEQ ID No. 12) used for detecting the number rs72014506 insertion / deletion polymorphic site on anMIR155HG gene, a specific primer pair (SEQ ID No. 13-SEQ ID No. 18) used for detecting the number rs113044851 insertion / deletion polymorphic site on a CTH gene and a specific primer pair (SEQ ID No. 19-SEQ ID No. 24) used for detecting the number rs397729601 insertion / deletion polymorphic site on a DSG2 gene. The kit has the advantages that the SCD risks of a patient are evaluated through the insertion / deletion polymorphic evaluation, the associated analysis of the genotype of the four sites can favorably provide molecular-genetics support and help for the diagnosis and prediction of the SCD,and the kit has high application value in the risk evaluation and diagnosis of the SCD; in addition, the kit is good in detection specificity, high in sensitivity and good in accuracy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for diagnosing / predicting sudden cardiac death. Background technique [0002] Sudden cardiac death (Sudden Cardiac Death, SCD) refers to sudden unexpected death caused by cardiac causes, manifested as sudden loss of consciousness and sudden circulatory and respiratory arrest in a short period of time. The specified time limit of "short term" is <1 hour when there are eyewitnesses, and 24 hours when there are no eyewitnesses. Epidemiological studies have shown that the incidence of SCD in the Chinese population is about 0.42‰, which is comparable to that of other Asian populations (0.40‰), and slightly lower than that of European and North American populations (0.5‰-1.0‰). However, due to the large population in China, there are far more SCD cases than in other countries. Due to its unexpected onset, rapid disease progression and sudden death, SCD seriously threatens human ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 高玉振何艳李立娟张晴尹志霞周玮邹燕
Owner SUZHOU UNIV
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