Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application

A technology for polymorphism detection and detection methods, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of undiscovered patent documents, etc., to improve the speed of breeding of improved varieties, low cost effect

Active Publication Date: 2020-05-01
天津奥群牧业有限公司
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[0006] Through searching, no published patent documents related to this patent application have been found

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  • Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application
  • Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application
  • Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application

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Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that this embodiment is descriptive and not restrictive, and the protection scope of the present invention cannot be limited by this.

[0031] The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention, unless otherwise specified, are conventional methods in the field.

[0032] A primer pair for detecting insertion / deletion polymorphism of sheep RORA gene, the primer pair can be used for PCR amplification of the 23-bp insertion / deletion polymorphism site of sheep RORA gene NC_040258.1:S.10400_10423.

[0033] Preferably, the primer pair is:

[0034] Upstream primer: SEQ NO.1 5'-GGATGGGGCTTGGTGGATTA-3' (20nt);

[0035] Downstream primer: SEQ NO. 2 5'-CAGGTGGTGAGCCATCTTGG-3' (20nt).

[0036] The application of the primer pair for detecting the insertion / deletion polymorphism of the sheep RORA gen...

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Abstract

The invention relates to a detection method for insertion / deletion polymorphism of a sheep RORA (retinoid acid receptor related orphan receptor A) gene. The detection method comprises the following steps: by taking genome DNA (deoxyribonucleic acid) of sheep to be detected as a template, and a primer pair as amplification primers, performing PCR (polymerase chain reaction) amplification on a fragment with insertion / deletion polymorphic sites in a sheep RORA gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion / deletion polymorphic sites according to electrophoresis results, wherein the insertion / deletion polymorphic sites are selected from 23-bp insertion / deletion polymorphic sites of NC_040258.1:S.10400_10423 sites of the sheep RORA gene. By adopting the method, the genotypes of the insertion / deletion polymorphic sites can be simply, rapidly and precisely detected with low cost.

Description

Technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a detection method, primer pair and application of sheep RORA gene insertion / deletion polymorphism. Background technique [0002] Sheep are deeply loved by consumers for its meat, milk and cashmere properties. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is increasing. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the genetic structure and function of sheep, and through screening and sheep production DNA marker sites closely related to litter number traits are detected and tested, and based on the association between genetic polymorphisms and litter number traits, the establishment of sheep populations with excel...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张清峰杨钰塔程志强潘传英林春建韩旭飞卢小芳蓝贤勇
Owner 天津奥群牧业有限公司
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