Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application
A technology for polymorphism detection and detection methods, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of undiscovered patent documents, etc., to improve the speed of breeding of improved varieties, low cost effect
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[0030] The embodiments of the present invention will be described in detail below. It should be noted that this embodiment is descriptive and not restrictive, and the protection scope of the present invention cannot be limited by this.
[0031] The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention, unless otherwise specified, are conventional methods in the field.
[0032] A primer pair for detecting insertion / deletion polymorphism of sheep RORA gene, the primer pair can be used for PCR amplification of the 23-bp insertion / deletion polymorphism site of sheep RORA gene NC_040258.1:S.10400_10423.
[0033] Preferably, the primer pair is:
[0034] Upstream primer: SEQ NO.1 5'-GGATGGGGCTTGGTGGATTA-3' (20nt);
[0035] Downstream primer: SEQ NO. 2 5'-CAGGTGGTGAGCCATCTTGG-3' (20nt).
[0036] The application of the primer pair for detecting the insertion / deletion polymorphism of the sheep RORA gen...
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