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A detection method and application of boar kdm5b gene insertion/deletion polymorphism

A detection method and polymorphic site technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as lack of research, influence of boar reproductive traits, and blank correlation studies, and achieve low cost, The effect of speeding up the breeding of fine varieties

Inactive Publication Date: 2020-04-28
NORTHWEST A & F UNIV
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Problems solved by technology

[0016] In summary, the KDM5B gene may have an impact on the reproductive traits of boars
At present, the research on the KDM5B gene in the field of genetic variation in boars is scarce, and the research on the association between the gene Indel and reproductive traits is even more blank

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  • A detection method and application of boar kdm5b gene insertion/deletion polymorphism
  • A detection method and application of boar kdm5b gene insertion/deletion polymorphism
  • A detection method and application of boar kdm5b gene insertion/deletion polymorphism

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Embodiment Construction

[0037] The present invention utilizes PCR amplification method to detect the insertion / deletion polymorphism that boar KDM5B gene may produce at NC_010452.3:g. Long, short axis length of testis) for association analysis to verify whether it can be used as a molecular marker for assisted selection in molecular breeding of boars. The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

[0038] 1. Experimental drugs and reagents

[0039] 1.1 Biochemical and biological reagents: ①Taq DNA polymerase (purchased from Fermantas, MBI); ②Proteinase K (purchased from Huamei Bioengineering Company); ③Marker (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.).

[0040] 1.2 Ordinary reagents: Ordinary reagents are purchased from Huamei Bioengineering Co., Ltd., and are packaged for imports: Tris, EDTA, NaCl, NaOH, KCl, NaCl 2 HPO 4 、KH 2...

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Abstract

The invention discloses a detection method of boar KDM5B gene insertion / deletion polymorphism and application. The detection method comprises the following steps: adopting a boar whole genome DNA as a template, amplifying KDM5B genes by virtue of PCR, then performing agarose gel electrophoresis, and identifying 35-bp insertion / deletion polymorphism of the KDM5B gene at a NC_010452.3: g.52599_52633 site. Different gene types of the 35-bp insertion / deletion polymorphism of the KDM5B gene are significantly related to reproductive traits such as short testicle axial length, heavy testicle and the like, the KDM5B gene 35-bp insertion / deletion polymorphism site can be used as a molecular marker for improving the reproductive traits of the Landrace boar, so that the marker assisted selection (MAS) of the Landrace boar reproductive traits is facilitated, and a boar population with excellent genetic resources can be rapidly established.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion polymorphism, in particular to a method for rapidly and accurately detecting the 35-bp insertion / deletion polymorphism of boar KDM5B gene NC_010452.3:g.52599_52633 site sexual method. Background technique [0002] As the most important meat consumption product for Chinese people, pigs have a huge stock in China. Breeding germplasm resources with excellent production traits is the guarantee for sufficient pork supply. By studying the genetic information of the pig genome, we can find polymorphisms in genes related to reproductive performance, resistance, animal product quality, feed conversion rate, etc., and apply these polymorphic information in the field of pig molecular breeding, which is conducive to breeding and improvement Produce more varieties with excellent production performance. [0003] Pig is an important m...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 潘传英崔洋于帅陈瑞曾文先董武子
Owner NORTHWEST A & F UNIV
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