A detection method and application of boar kdm5b gene insertion/deletion polymorphism

A detection method and polymorphic site technology, applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as lack of research, influence of boar reproductive traits, and blank correlation studies, and achieve low cost, The effect of speeding up the breeding of fine varieties
CN106755474BInactive Publication Date: 2020-04-28NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2020-04-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection method of boar KDM5B gene insertion / deletion polymorphism and application. The detection method comprises the following steps: adopting a boar whole genome DNA as a template, amplifying KDM5B genes by virtue of PCR, then performing agarose gel electrophoresis, and identifying 35-bp insertion / deletion polymorphism of the KDM5B gene at a NC_010452.3: g.52599_52633 site. Different gene types of the 35-bp insertion / deletion polymorphism of the KDM5B gene are significantly related to reproductive traits such as short testicle axial length, heavy testicle and the like, the KDM5B gene 35-bp insertion / deletion polymorphism site can be used as a molecular marker for improving the reproductive traits of the Landrace boar, so that the marker assisted selection (MAS) of the Landrace boar reproductive traits is facilitated, and a boar population with excellent genetic resources can be rapidly established.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion polymorphism, in particular to a method for rapidly and accurately detecting the 35-bp insertion / deletion polymorphism of boar KDM5B gene NC_010452.3:g.52599_52633 site sexual method. Background technique

[0002] As the most important meat consumption product for Chinese people, pigs have a huge stock in China. Breeding germplasm resources with excellent production traits is the guarantee for sufficient pork supply. By studying the genetic information of the pig genome, we can find polymorphisms in genes related to reproductive performance, resistance, animal product quality, feed conversion rate, etc., and apply these polymorphic information in the field of pig molecular breeding, which is conducive to breeding and improvement Produce more varieties with excellent production performance.

[0003] Pig is an important m...

Claims

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