32 results about "Landrace pig" patented technology

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing the content of porcine inosine and application of the SNP marker. The 10 SNP molecular markers closely related to the porcine inosine content are researched and determined on the basis of American-series purebred landrace, American-series purebred large white pigs and American-series purebred duroc, the dominant allele frequency can be increased generation by generation by optimizing the dominant allele of the SNP, the inosine content can be reduced, and the molecular markers can be used for identifying the porcine inosine content. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with correction of a 100kg backfat thickness character of a pig and application of the SNP molecular marker. The SNP molecular marker is located on a pig chromosome 2 and contains six SNP molecular marker sites. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out in combination with the 100kg backfat thickness correction character of the landrace, six SNP molecular markers obviously related to the character are screened out, and a new marker resource is provided for molecular breeding of the growth character of the boar.

The invention provides an SNP (Single Nucleotide Polymorphism) marker primer pair related to pig multirib traits and application of the SNP marker primer pair. The SNP site is a nucleotide site of chromosome 7 rs701714758 of a reference sequence of an international pig genome version 11.1, has G / A polymorphism, is located in a protein coding region of an ABCD4 gene, and is remarkably associated with rib number characters of Duroc pigs, landrace pigs, large white pigs and Pietrain pigs through verification of whole genome association analysis and meta analysis. The invention further provides a primer pair for detecting the SNP marker, an upstream primer of the primer pair is SEQ ID NO: 2, and a downstream primer of the primer pair is SEQ ID NO: 3. The SNP marker can be applied to marker-assisted selection and whole genome selection of rib number characters of multiple varieties of pigs, can accurately screen a multi-rib number pig group, improves character prediction accuracy, increases pork and rib row yield, meets consumption demands, and has both social and economic benefits.

The application relates to the technical field of pig breeding, and discloses a breeding method of a grain-saving and environment-friendly type pig, which comprises the following steps: S1, respectively establishing a Duroc pig basic group, a Landrace pig basic group and a Large Yorkshire pig basic group; S2, breeding the pig basic groups in the step S1 by combining variety matching with performance measurement, and respectively establishing a Duroc pig core group, a Landrace pig core group and a Large Yorkshire pig core group; S3, selecting boars and sows from the Duroc pig core group, the Landrace pig core group and the Large Yorkshire pig core group, and performing mating test according to breeding requirements; S4, feeding the offspring of the core groups with the same feed, starting weight and feed consumption measurement when the weight is 25-35 kg, ending when the weight is 95-105 kg, and making records; and S5, repeatedly selecting and breeding in an optimal manner. The application can save breeding cost and feed resources, and reduce greenhouse gas emission and manure pollution.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker capable of influencing the content of IMP or GMP (Good Manufacturing Practice) of pigs and application of the SNP marker. According to the invention, based on three test swinery of American-series purebred landrace, American-series purebred large white pig and American-series purebred Duroc, pig whole genome re-sequencing and GWAS analysis are adopted, 15 SNP markers related to the content of IMP or GMP are researched and determined, and by optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the content of IMP or GMP can be increased, and the number of SNP markers related to the content of IMP or GMP can be increased. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to an application of 3-phenylpropionic acid in increasing the fat content in pig muscle. According to the invention, the 3-phenylpropionic acid is added into the pig daily ration, so that the intramuscular fat content of the landrace can be remarkably increased, and the quality of the pork, such as taste, flavor, succulent meat quality and the like, is further improved. The invention discloses an important role of 3-PPA in regulation and control of fat deposition in pig muscle, and provides a new nutrition regulation and control target and a genetic breeding strategy for improvement of pork quality.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting the total flavor nucleotide content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify nine SNP markers associated with total flavor nucleotides. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing the total flavor nucleotide content, improving pork quality, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The application discloses a SNP molecular marker of a site rs338618851 related to pork production efficiency of pigs and application. The genetic marker is cloned from a 93991071 site of a chromosome 6 of pigs. American Landrace pigs are selected as test materials, whole genome DNA is extracted from blood of the pigs, and a primer is designed according to a pig genome sequence NC_010448.4 published in an NCBI database. A base substitution of T or C exists at a 172th position of a primer amplification sequence, the substitution causes polymorphism at the position. The SNP site is typed through a direct sequencing technology, and correlation analysis shows that, compared with other gene individuals, a TT genotype individual at the 172th position is large in birth weight, large in eye muscle area and short in time to reach 100 kg at a certain age. The application provides a new marker for molecular breeding of pork production traits of pigs.

The present invention belongs to the field of molecular biotechnology and molecular marker technology, and discloses a SNP molecular marker related to pig birth weight and its application. The SNP molecular marker related to pig birth weight corresponds to the nucleic acid site at 5379864 bp on chromosome 16 of the pig reference genome Sscrofa11.1 version sequence. The base of the site is C or T and is contained in the intron of the MARCHF11 gene. The present invention also discloses the application of the SNP molecular marker related to pig birth weight in the genetic breeding of Landrace pigs, and provides a method for selecting or assisting in the selection of pigs with high / low birth weight using the SNP molecular marker and a primer for identifying the SNP site. The above method can achieve accurate prediction and efficient selection of Landrace pig birth weight, promote the cultivation of excellent breeding pigs, accelerate the population improvement process, and provide strong support for the sustainable development of the pig farming industry.

The invention discloses a functional site combination for predicting sow reproductive traits and application thereof.The functional site combination comprises 12232 SNP loci, ear tissues of Changbai breeding pigs from a certain pig farm in the south of China are collected, gene chip sequencing and whole genome re-sequencing data are used, and the functional site combination is used for predicting the reproductive traits of sows. A large number of SNP markers are obtained by using a genotype filling technology, functional marker information related to the breeding traits of the landrace sows is screened in combination with multi-omics information, noise sites in filling data are removed, and finally the 10K functional site applied to the breeding traits of the landrace sows is formed. The SNP molecular marker related to the breeding traits of the landrace sows is screened out, a new molecular marker development basis is provided for prediction and genetic improvement of the breeding traits related to the landrace sows, and the SNP molecular marker has important significance in improving the breeding and breeding efficiency of pigs.

The application discloses a binary pig efficient breeding method based on hybridization advantage and application, relates to the technical field of livestock breeding, and comprises the following steps: acquiring phenotype data of pure line Landrace pigs, pure line Large White pigs and historical hybrid offspring, setting the weight of reproduction traits, growth traits and feed efficiency traits in combination with external environment parameters, and generating comprehensive heterosis evaluation indexes; performing high-density genotyping on candidate Landrace pigs and candidate Large White pigs, constructing a genetic effect prediction model based on the genotyping results and the phenotype data, predicting the hybridization effect of a pairing combination of Landrace pigs and Large White pigs by using the genetic effect prediction model, screening the pairing combination meeting the comprehensive heterosis evaluation indexes according to the hybridization effect, and implementing artificial insemination to obtain first-generation binary pigs; and performing whole life cycle performance determination on the first-generation binary pigs, and calculating the measured comprehensive heterosis evaluation indexes.

The application discloses a SNP molecular marker related to a total number of piglets character of a Changbai pig and application thereof, a SNP site with a login number of rs322407989 is significantly related to the total number of piglets character, and the SNP site is located at a base at position 92518039 of a chromosome 7 (version 11.1 of a pig genome), reference is made to an Ensembl database, nucleotide sequences of 50 bp upstream and downstream of a fragment with the login number of rs322407989 are obtained, the nucleotide sequences are shown in a sequence table SEQ ID NO. 1 or 2, a polymorphism site is G or A, compared with an individual with a genotype of GG, the Changbai pig has a higher total number of piglets when the genotype is AG.

The application belongs to the technical field of molecular genetics, and provides a SNP molecular marker related to pig backfat thickness and application thereof. The application studies and determines that the pig backfat thickness related molecular marker is located at the 9558333th site of chromosome 18 in the international pig reference genome 11.1 version (corresponding to the 701th base A / G mutation of SEQ ID NO:1), and verifies the influence effect on the pig backfat thickness. When the base of the site is A, the pig has lower backfat thickness. Based on this, the application establishes a molecular marker assisted selection breeding technology for rapidly improving the pig backfat thickness, and the frequency of the advantageous allele (A) can be increased generation by generation, the backfat thickness of the breeding pig is significantly reduced, the genetic improvement process of the commercial pig population containing the bloodline of the Landrace pig and the Large White pig is accelerated, the breeding efficiency is improved, the sales profit of the enterprise is increased, the core competitiveness is improved, and the economic benefit of the commercial pig is improved.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects porcine inosine content and its application. Based on purebred American Landrace, purebred American Large White, and purebred American Duroc pigs, this invention studies and identifies 10 SNP molecular markers closely related to porcine inosine content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, reducing inosine content, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The present application belongs to the field of molecular biological technology and molecular marker technology, and relates to a SNP molecular marker related to pork yield efficiency of pigs and application thereof, which is from the 93707553th site of chromosome 15 of pigs. Large white pigs and long white pigs are selected as test materials, whole genome DNA is extracted from blood of the pigs, and primers are designed according to the pig genome sequence (NC_010457.5) published by NCBI database. There is a base substitution of A or G at the 306th site, resulting in polymorphism. The SNP site is typed, and correlation analysis shows that, compared with other gene individuals, the AA genotype individuals have the traits of thin backfat in vivo, large eye muscle area and the like. The present application provides a new marker for pig molecular marker assisted selection, and the marker is applied to genetic improvement of pork yield efficiency of pigs, so that the breeding improvement process of high-quality pigs is accelerated.

The invention relates to the field of gene molecular breeding, and discloses a Landrace pig growth and reproduction SNP molecular marker and application thereof, and is characterized in that the Landrace pig SNP molecular marker is composed of 306 SNP molecular markers, and the physical positions of the 306 SNP molecular markers are determined by sequence alignment based on a pig reference genome Sscrofa11.1; the site information of the SNP molecular marker is specifically shown in the specification table 1. The Landrace growth and reproduction SNP molecular marker has the beneficial effects that the Landrace growth and reproduction SNP molecular marker provided by the invention can be used for low-cost and large-scale genetic typing of the Landrace, the sites are flexible based on a liquid chip technology, and new functional marker sites can be added at any time in the later period.

The invention belongs to the technical field of gene detection, and relates to a detection method of nucleic acid, in particular to a pig breed identification method based on a liquid chip technology and application of the pig breed identification method. According to the invention, a specific SNP marker combination aiming at 73 pig varieties is established for the first time, the specific SNP marker combination is used for constructing a liquid phase chip for pig variety identification, and targeted capture and high-throughput sequencing are combined, so that the distinguishing accuracy of 95% or more among the 73 pig varieties is successfully realized. The method disclosed by the invention has the advantages of wide variety types, high cost performance, high accuracy and short detection period, and can be used for identifying the variety of landrace, large white pig, duroc, 28 wild boars and 42 domestic pigs.

The invention discloses a disease-resistant cell strain, a section of amino acid sequence of M130 protein expressed in the cell strain is deleted to generate a new M130p.Ser1028Gly1041del protein type, the amino acid sequence of the M130p.Ser1028Gly1041del protein type is as shown in SEQ ID No: 28, and the cell strain has the capability of resisting PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). The cell nucleus of the cell strain can be used as a donor to produce a disease-resistant cloned pig, and complete resistance to the current PRRSV epidemic strain is obtained. Through molecular design and a new editing method, natural mutation of animal endogenous genes is modified under the condition that the M130 endogenous protein space structure and the biological function are not affected, and the generated gene editing pig is better in health degree, high in cloning efficiency and higher in biological safety, and can be used for preparing the M130 endogenous protein. The method provided by the invention can be applied to the creation of novel materials for resisting the porcine reproductive and respiratory syndrome, such as large white pigs, landrace pigs, duroc pigs, Berkshire pigs, Hanpshire pigs and Pietrain pigs of different strains, and local pigs in China, and lays a foundation for breeding and popularization of porcine reproductive and respiratory syndrome resisting varieties.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker influencing the pig AMP (Adenosine monophosphate) content and application thereof. On the basis of three test swinery of American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred Duroc, pig whole genome re-sequencing and GWAS analysis research are adopted, 14 SNP molecular markers remarkably related to the AMP content are determined, dominant alleles of the SNP are optimized, the frequency of the dominant alleles can be increased generation by generation, the AMP content can be increased, the number of the SNP molecular markers is increased, and the number of the SNP molecular markers is increased. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker capable of influencing the content of porcine purine bases and application of the SNP marker. According to the invention, based on three test swinery of American-series purebred landrace, American-series purebred large white pig and American-series purebred Duroc, pig whole genome re-sequencing and GWAS analysis are adopted, 15 SNP markers which significantly influence the content of pig purine bases are researched and determined, and by optimizing the dominant alleles of the SNP markers, the frequency of the dominant alleles can be increased generation by generation, and the number of the dominant alleles can be increased. The corresponding purine content is reduced or increased, and the genetic improvement progress of pigs is accelerated, so that the economic benefit of breeding of boars is effectively improved.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and discloses a pig birth weight related SNP (single nucleotide polymorphism) molecular marker and application thereof, the pig birth weight related SNP molecular marker corresponds to a nucleic acid site at 5379864 bp of chromosome 16 of a pig reference genome Sscrofa11.1 version sequence, and a basic group of the site is C or T and is contained in an intron of an MARCHF11 gene. The invention also discloses an application of the SNP molecular marker related to the birth weight of the pig in genetic breeding of landrace pigs, and provides a method for breeding or assisted breeding of pigs with high / low birth weight by using the SNP molecular marker and a primer for identifying the SNP site. By means of the method, precise prediction and efficient selection of the birth weight of the landrace can be achieved, breeding of excellent breeding pigs is promoted, the population improvement process is accelerated, and powerful support is provided for sustainable development of the pig breeding industry.

The application relates to the field of gene molecular breeding, discloses a long white pig growth and reproduction SNP molecular marker and application thereof, and is characterized in that the long white pig SNP molecular marker is composed of 306 SNP molecular markers, the physical position of the 306 SNP molecular markers is determined based on sequence alignment of a pig reference genome Sscrofa11.1; and the SNP molecular marker site information is specifically shown in Table 1 in the description. The long white pig growth and reproduction SNP molecular marker provided by the application can be used for low-cost and large-scale genotyping of long white pigs, is based on liquid phase chip technology, is flexible in site, and can increase new functional marker sites at any time in the later period.

The present invention relates to a method for efficiently extracting porcine hair DNA, comprising the following steps: hair follicle collection, hair follicle digestion, DNA extraction, precipitation and washing. The DNA extraction is carried out twice. The first extraction is with Tris-saturated phenol, and the second extraction is with a mixture of saturated phenol: chloroform: isoamyl alcohol = 25:24:1. Then, isopropanol and 10 M ammonium acetate are added to the secondary extraction solution for the first precipitation of DNA, and ice ethanol is added to the precipitate for the second precipitation of DNA, followed by subsequent washing and other treatments. Using the method of the present invention, porcine hair DNA of Duroc, Large White pigs and Landrace pigs can be quickly and effectively extracted, and the extraction quality meets the requirements of DNA for gene chip sequencing.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects AMP content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention uses whole-genome resequencing and GWAS analysis to identify 14 SNP molecular markers significantly associated with AMP content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing AMP content, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) marker capable of influencing the total flavor nucleotide content of pigs and application of the SNP marker. According to the invention, nine SNP markers related to total flavor nucleotides are researched and determined by adopting pig whole genome re-sequencing and GWAS analysis on the basis of three test swines of American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred Duroc, and dominant alleles of the SNP are optimized, so that the frequency of the dominant alleles can be increased generation by generation, and the number of the dominant alleles can be increased. The total flavor nucleotide content is increased, the pork quality is improved, and the genetic improvement progress of pigs is accelerated, so that the economic benefit of breeding of boars is effectively improved.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker that affects the IMP or GMP content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify 15 SNP markers associated with IMP or GMP content. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing IMP or GMP content, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

The invention discloses a lean type pig breeding method which comprises the following steps: S1, basic group construction: a female parent group: on the basis of Suzhong pig line 1 and Suzhong pig line 2, introducing 15-20% of brown-hair glutinous grain pig blood lineage to form a synthetic line female parent group; in the male parent group, 10-15% of Duroc pig blood lineage is introduced on the basis of British large white pigs to form a synthetic line male parent group; s2, crossbreeding: adopting a four-way hybridization mode: in the first round of hybridization, synthetic line female parent group female * dan line landrace male to F1 generation sows; the second round of hybridization: F1-generation sows * synthetic line male parent groups are male parent groups and breeding groups are obtained; s3, precise breeding is conducted in four stages; S4, generation breeding is conducted, wherein a 2 + 3 breeding mode is adopted, and two generations are bred in a closed mode to fix good characters; and 3 exogenous boars with blood lineage are introduced in an open manner for blood relationship renewal. The breeding performance, the growth speed, the lean meat percentage and the meat quality character can be improved at the same time.