Application and method of sheep KDM3B gene insertion/deletion as early selection of reproductive traits

Abstract

The invention relates to a sheep KDM3B gene insertion / deletion polymorphism detection method, which comprises the following steps of adopting sheep genome DNA to be detected as a template, carrying out PCR amplification on an intron region insertion / deletion polymorphism site containing a sheep KDM3B gene, carrying out electrophoresis typing on the amplification product, and identifying the genotype corresponding to the insertion / deletion polymorphism site according to the electrophoresis result, wherein the insertion / deletion polymorphic site is selected from a 7-bp indel polymorphic site at the site of the sheep KDM3B gene NC_040256.1:g.52149274-52149280. According to the method, the genotype of the insertion / deletion polymorphic site can be simply, quickly and accurately detected with low cost, so that the Australian white sheep genetic resource population with excellent propagation traits can be quickly established, and the sheep population with excellent propagation genetic resources can be quickly established.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to an application method of sheep KDM3B gene insertion / deletion (indel) as early selection of reproductive traits. Background technique [0002] As one of the earliest animals domesticated by humans, sheep have important economic value in the animal husbandry industry and can provide people with a series of animal by-products such as mutton, goat milk, cashmere, and sheepskin. my country is the country with the largest amount of sheep breeding, slaughtering and mutton output. The reproductive traits of sheep directly affect the production efficiency of breeding enterprises. Modern sheep breeding aims to improve its production and reproductive performance thereby greatly increasing its economic value. With the development of biological science and technology and the deepening of genetic research, the traditional selection method has gradually exposed the...

AI Technical Summary

Problems solved by technology

With the in-depth study of comparative genomics, InDel has provided a large amount of biological information for theoretical research and genetic breeding application research. As a genetic identification marker, it is mostly concentrated in human genome research. There are few studies and applications of InDel markers that can be applied to the breeding of reproductive traits in ruminants

However, there is no report on the InDel site of the KDM3B gene and its relationship with reproductive traits

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