Application and method of sheep KDM3B gene insertion/deletion as early selection of reproductive traits

A sheep and gene technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of little research and application, and the InDel site of KDM3B gene has not been seen, so as to improve the selection of improved breeds The effect of breeding speed and low cost

Active Publication Date: 2021-04-06
天津奥群牧业有限公司
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Problems solved by technology

With the in-depth study of comparative genomics, InDel has provided a large amount of biological information for theoretical research and genetic breeding application research. As a genetic identification marker, it is mostly concentrated in human gen

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  • Application and method of sheep KDM3B gene insertion/deletion as early selection of reproductive traits
  • Application and method of sheep KDM3B gene insertion/deletion as early selection of reproductive traits
  • Application and method of sheep KDM3B gene insertion/deletion as early selection of reproductive traits

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Embodiment Construction

[0030] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0031] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0032] The 7-bp insertion / deletion polymorphism of sheep KDM3B gene NC_040256.1:g.52149274-52149280.

[0033] A detection method for insertion / deletion polymorphism of sheep KDM3B gene, comprising the following steps:

[0034] Using the sheep genomic DNA to be tested as a template, use PCR to amplify the insertion / deletion polymorphism site containing the intron region of the sheep KDM3B gene, perform electrophoresis typing ...

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Abstract

The invention relates to a sheep KDM3B gene insertion/deletion polymorphism detection method, which comprises the following steps of adopting sheep genome DNA to be detected as a template, carrying out PCR amplification on an intron region insertion/deletion polymorphism site containing a sheep KDM3B gene, carrying out electrophoresis typing on the amplification product, and identifying the genotype corresponding to the insertion/deletion polymorphism site according to the electrophoresis result, wherein the insertion/deletion polymorphic site is selected from a 7-bp indel polymorphic site at the site of the sheep KDM3B gene NC_040256.1:g.52149274-52149280. According to the method, the genotype of the insertion/deletion polymorphic site can be simply, quickly and accurately detected with low cost, so that the Australian white sheep genetic resource population with excellent propagation traits can be quickly established, and the sheep population with excellent propagation genetic resources can be quickly established.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to an application method of sheep KDM3B gene insertion / deletion (indel) as early selection of reproductive traits. Background technique [0002] As one of the earliest animals domesticated by humans, sheep have important economic value in the animal husbandry industry and can provide people with a series of animal by-products such as mutton, goat milk, cashmere, and sheepskin. my country is the country with the largest amount of sheep breeding, slaughtering and mutton output. The reproductive traits of sheep directly affect the production efficiency of breeding enterprises. Modern sheep breeding aims to improve its production and reproductive performance thereby greatly increasing its economic value. With the development of biological science and technology and the deepening of genetic research, the traditional selection method has gradually exposed the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 张清峰康雨欣潘传英蓝贤勇林春建苏鹏黄洋铭秦巧梅徐红伟李铭
Owner 天津奥群牧业有限公司
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