64 results about "Comparative genomics" patented technology

The invention relates to a haynaldia villosa's 6VS chromosome specific molecular marker 6VS-BH1 and an application thereof, and belongs to the technical fields of molecular biology and genetic breeding science. The labeled primer 6VS-BH1 is developed on the basis of wheat-brachypodium distachyon comparative genomics means, and the specific sequence is 6VS-BH1F, as shown in SEQ ID NO.1, and 6VS-BH1R, as shown in SEQ ID NO.2. The molecular marker 6VS-BH1 has the advantages of wide annealing temperature range (60-66 DEG C), good stability, strong product brightness, high resolution and the like. The molecular marker 6VS-BH1, as a co-dominant marker, not only can be used for effectively tracing haynaldia villosa's 6VS chromosome in wheat background, but also can be used for distinguishing a homozygote and heterozygote and for distinguishing a Pm21 gene carried translocation line T6VS.6AL and a PmV gene carried translocation line T6VS.6DL. Therefore, the molecular marker 6VS-BH1 disclosed by the invention has an important practical value in the molecular marker-assisted selection breeding of wheat powdery mildew and the pyramiding breeding of Pm21 gene and PmV gene.

The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.

A method and system for representing a similarity between at least two genomes that includes detecting gene clusters which are common to the at least two genomes and representing the common gene clusters in a PQ tree. The PQ tree includes a first internal node (P node), that allows permutation of the children thereof, and a second internal node (Q node), that maintains unidirectional order of the children thereof.

The invention provides transcription factors participating in regulating and controlling synthesis of bitter principles of cucumis melo and application of the transcription factors. A comparative genomics method is utilized for the first time, and the two bHLH transcription factors CmBr and CmBt for controlling synthesis of bitter taste are discovered in genomes of the cucumis melo, and separatelycontrol formation of the bitter taste in roots and wild fruits. Through a yeast one-hybrid technology, a gel retardation test and a tobacco transient expression system, it is proved that the two transcription factors can be directly combined with a promoter region of a bitter principle synthesis gene, and then the expression of the synthesis gene is activated; meanwhile, through transient expression of cotyledons of the cucumis melo, it is proved genetically that the overexpression of CmBr and CmBt can activate the expression of the bitter taste synthesis gene, so that the cotyledons withoutthe bitter taste obtain the phenotype of the bitter taste. The CmBt gene is located in a domestication region. The invention further discloses a molecular mechanism for formation of the bitter taste of the cucumis melo, and a theoretical basis is provided for breeding of the cucumis melo without the bitter taste.

The invention provides rapid identification of soybean anti-powdery mildew gene, relates to knowledge in the disciplines of plant comparative genomics, genetics and bioinformatics, and belongs to the scientific field of plant biotechnology. The invention includes the steps of: 1) downloading the soybean whole genome sequence and acquiring MLO type gene; 2) identifying the MLO genotype; 3) identifying MLO gene phylogenetic relationship; and 4) comparing MLO-type powdery mildew gene. The invention effectively shortens the gene mining cycle for soybean powdery mildew, and is conducive to the rapid identification of anti-powdery mildew gene; corresponding coseparation functional markers (SNP, scar, etc.) are developed through the identified candidate powdery mildew genes, and the identified candidate powdery mildew genes can also be used for fast molecular marker assisted selection of anti-powdery mildew genes and have high accuracy; and combined with other resistant gene molecular markers, multi-resistance breeding material can be created, so as to shorten the breeding period and improve breeding efficiency. Therefore, the invention lays foundation for the explanation of powdery mildew resistant molecular mechanism of soybean.

The invention provides a transcription factor participating into regulation of muskmelon bitter principle synthesis and application of the transcription factor. A comparative genomics method is firstly utilized for finding two bHLH transcription factors CmBr and CmBt which control bitter synthesis in a muskmelon genome, the two bHLH transcription factors are respectively used for controlling the bitter formation at the root part and in the wild fruit. A yeast one-hybrid technology, a gel retardant experiment and a tobacco transient expression system are used for proving that the two transcription factors can be directly combined to a promoter region of a bitter principle synthesis gene, and can activate the expression of the synthesis gene; the muskmelon cotyledon transient expression proves that the overexpression of each of the CmBr and the CmBt can activate the expression of the bitter synthesis gene from the genetics, so that the bitter-free cotyledon can acquire the bitter phenotype. The CmBt gene is located in a domestication region. The invention further discloses a molecule mechanism of the muskmelon bitter formation, and provides the theoretical basis for the breeding of the bitter-free muskmelon.

The invention belongs to the technical field of livestock molecular biology, and particularly relates to molecular cloning and application of pork quality character related gene Myo6 (Myosin 6). The molecular marker disclosed by the invention is obtained through cloning the Myo6 gene, and the sequence of the Myo6 molecular marker is described as SEQ ID NO: 1. The polymorphism of the Myo6 gene is as follows: a primer is designed by using the comparative genomics method according to the My06 gene sequence of human, the genome DNA of pigs is adopted as a template for amplification, SNP is screened by sequencing the amplified fragments, genotyping is carried out by using PCR-RFLP, a G / A base mutation at 709bp is discovered, and PCR-RFLP-Xba I polymorphism is caused. The molecular cloning and application of pork quality character related gene Myo6 provide a novel molecular marker for the assistant selection of pig markers.

Relating to plant comparative genomics, genetics, bioinformatics and other disciplinary knowledge, the invention belongs to the scientific field of plant biotechnology, and discloses rapid identification of phaseolus vulgaris mildew resistance locus o gene. The main steps include: 1) download of phaseolus vulgaris whole genome sequence and acquisition of MLO type gene; 2) identification of MLO type gene; 3) MLO type gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew. The invention effectively shortens the mining cycle of phaseolus vulgaris powdery mildew gene, and is conducive to rapid identification of powdery mildew gene. Corresponding coseparation functional markers (SNP, SCAR and the like) are developed through identified candidate powdery mildew genes, which also can be used for molecular marker-assisted selection of mildew resistance locus o gene and have high accuracy. Multi-resistance breeding materials can be created by combining other resistant gene molecular markers, the breeding period can be shortened, and the breeding efficiency is improved, thus laying the foundation for elaborating the phaseolus vulgaris mildew resistance molecular mechanism.

The invention belongs to the technical field of livestock molecular biology, and particularly relates to molecular cloning and application of a pig backfat thickness related SLC13A5 gene. A molecular marker disclosed by the invention is obtained by SLC13A5 gene cloning, and a sequence marked by an SLC13A5 molecule is shown in SEQ ID NO:1. According to the polymorphism of the SLC13A5 gene, a primer is designed according to SLC13A5 gene sequence of human by using a comparative genomic method and amplified by taking pig genome DNA as a template; SNP of the amplified fragment is screened in a sequencing manner; PCR-RFLP is utilized to implement genetic typing; base mutation of one T / C at the 251bp is found, which causes PCR-RFLP-Bsu36I polymorphism; moreover, the marker is used for detecting the conditions of Chinese and foreign pig breeds. The invention provides new molecular marking for pig marker assisted selection.