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Rapid identification of powdery mildew resistant gene of Chinese cabbage by using comparative genomics

A powdery mildew resistance gene and powdery mildew resistance technology, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problems of long time and difficulty in cloning, and achieve the effects of shortening time, shortening excavation cycle, and improving identification efficiency.

Inactive Publication Date: 2015-05-06
JIANGSU CHANGSHU MODERN AGRI IND PARK DEV
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Problems solved by technology

However, because the basic research of Chinese cabbage is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of powdery mildew resistant gene of Chinese cabbage by using comparative genomics
  • Rapid identification of powdery mildew resistant gene of Chinese cabbage by using comparative genomics
  • Rapid identification of powdery mildew resistant gene of Chinese cabbage by using comparative genomics

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Embodiment Construction

[0029]The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the powdery mildew gene of Chinese cabbage. The specific implementation process is as follows:

[0030] 1) Collection of MLO-type genes in Chinese cabbage

[0031] In order to obtain all the members of the MLO gene family in Chinese cabbage, we first constructed an HMM model based on the MLO genes of Arabidopsis thaliana and the MLO gene sequences of resistance to powdery mildew in tomato, pea, rose, pepper, and japonicus. Retrieve the MLO gene in different crops; secondly, use the published MLO gene sequence in different crops as the target sequence (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325904, TC3159 ,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC2...

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Abstract

The invention provides rapid identification of powdery mildew resistant gene of Chinese cabbage, relates to knowledge in the disciplines of plant comparative genomics, genetics and bioinformatics, and belongs to the scientific field of plant biotechnology. The invention includes the steps of: 1) downloading the whole genome sequence of Chinese cabbage and acquiring MLO type gene; 2) identifying the MLO genotype; 3) identifying MLO gene phylogenetic relationship; and 4) comparing MLO-type powdery mildew gene. The invention effectively shortens the gene mining cycle for Chinese cabbage powdery mildew, and is conducive to the rapid identification of powdery mildew resistant gene; corresponding coseparation functional markers (SNP, SCAR, etc.) are developed through the identified candidate powdery mildew genes, and the identified candidate powdery mildew genes can also be used for fast molecular marker assisted selection of powdery mildew resistant genes and have high accuracy; combined with other resistant gene molecular markers, multi-resistance breeding material can be created, so as to shorten the breeding period and improve breeding efficiency. Therefore, the invention lays foundation for the explanation of powdery mildew resistant molecular mechanism of Chinese cabbage.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategies to quickly identify Chinese cabbage powdery mildew genes by means of sequencing the entire genome sequence of Chinese cabbage, and mainly involves the download of the entire genome sequence of Chinese cabbage. Gene identification, gene comparison, clustering and other means to identify the powdery mildew gene belong to the field of plant biotechnology science. Background technique [0002] Chinese cabbage is one of the widely grown vegetable crops in China. In recent years, with global warming, the disease has become more and more serious in Northwest my country and North China, resulting in a sharp decline in the yield and quality of cabbage. It has become one of the main diseases in cabbage production. Spraying fungicides and planting resistant varieties are the main defenses against the disease. However, long-term use of f...

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68
Inventor 钱春桃其他发明人请求不公开姓名
Owner JIANGSU CHANGSHU MODERN AGRI IND PARK DEV
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