Rapid identification of corn powdery mildew resistant genes

A powdery mildew resistant gene and powdery mildew resistant technology is applied in genetic engineering, plant genetic improvement, microbial measurement/inspection, etc. It can solve the problems of difficult gene full-length sequences and short target sequences, shortening the mining cycle, The effect of shortening time and improving identification efficiency

Inactive Publication Date: 2015-04-29
南农大(常熟)新农村发展研究院有限公司
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Problems solved by technology

However, under normal circumstances, the target sequences obtained by the above techniques are relatively short, and it is generally not easy to directly obtain the full-length sequence of the gene

Method used

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  • Rapid identification of corn powdery mildew resistant genes
  • Rapid identification of corn powdery mildew resistant genes
  • Rapid identification of corn powdery mildew resistant genes

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Embodiment Construction

[0029] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the corn powdery mildew gene. The specific implementation process is as follows:

[0030] 1) Collection of maize MLO gene

[0031]In order to obtain all the MLO gene family members of maize, we first constructed an HMM model using the MLO gene sequences of Arabidopsis, tomato, pea, pepper, rose, pepper, and lotus root MLO gene sequences for resistance to powdery mildew. Retrieve the MLO gene in different crops; secondly, use the published MLO gene sequence in different crops as the target sequence (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325904, TC3159 ,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC285032,TC290021,TC302716,TC283487,TC...

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Abstract

The invention provides a rapid identification method of corn powdery mildew resistant genes and relates to plant comparative genomics. Such subject knowledge as genetics and bioinformatics belong to the field of plant biotechnology science. The method provided by the invention comprises the following main steps: 1) downloading a corn whole genome sequence and collecting an MLO gene; 2) verifying the MLO gene; 3) researching the phylogenetic relationship of the MLO gene; and 4) comparing MLO type powdery mildew genes. By adopting the invention, the mining cycle of the corn powdery mildew genes is effectively shortened to facilitate the rapid identification of the powdery mildew genes; corresponding co-separation functional markers (SNP, SCAR and the like) are developed via the identified powdery mildew genes, and the identified powdery mildew genes can also be quickly applied to the auxiliary selection of the molecular markers of the powdery mildew resistant genes, and the accuracy is high; in combination with other anti-disease gene molecular markers, a multi-resistance breeding material can be cultured to shorten the breeding period and improve the breeding efficiency; a foundation is laid for illustrating the corn powdery mildew resistant molecular mechanism.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategies to quickly identify corn powdery mildew genes by means of the whole genome sequence of corn, and mainly involves the download of the whole genome sequence of corn and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Powdery mildew is a worldwide plant disease that affects most plants. It occurs in plant leaves, tender stems, flower stalks, flower buds, petals and other parts. The initial stage is yellow-green irregular small spots with inconspicuous edges. Soon the lesions continued to expand, white powder spots appeared on the surface, and finally numerous black spots grew on the place, and the affected parts turned gray, covering the surface in a continuous p...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/29
CPCC07K14/415C12Q1/6895C12Q2600/156
Inventor 钱孝英钱春桃
Owner 南农大(常熟)新农村发展研究院有限公司
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