Loofah gummy stem blight-resistant gene segments or gene markers and application thereof

A gene marker and gene fragment technology, applied in the field of plant biotechnology science, can solve problems such as economic loss, affecting the growth and development process of loofah plants, and achieve the effects of shortening breeding years, improving breeding efficiency, and improving identification efficiency.

Inactive Publication Date: 2015-04-29
CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP
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  • Abstract
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Problems solved by technology

Blight has seriously affected the normal growth and development process of loofah plants, bringing great economic losses to production

Method used

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  • Loofah gummy stem blight-resistant gene segments or gene markers and application thereof
  • Loofah gummy stem blight-resistant gene segments or gene markers and application thereof
  • Loofah gummy stem blight-resistant gene segments or gene markers and application thereof

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Embodiment Construction

[0017] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the loofah resistance gene. The specific implementation process is as follows:

[0018] 1. DNA extraction: Take an appropriate amount of young leaves of loofah, dry them with silica gel, grind them into powder, and extract the total genomic DNA by CTAB method. The extracted DNA was detected by an ultraviolet spectrophotometer, and samples with an OD260 / OD280 value of 1.8-2.0 were selected and stored at -20°C for future use.

[0019] 2. Amplification and cloning of homologous fragments of disease resistance genes: according to the amino acid sequences GG(L / I / V / M / S)GKTT and G(L / N ) PL (A / T / S) (L / V), designed a pair of degenerate primers: NBS15'-GKTT GGIGGIWSIGGIAARACIACG-3' and NBS25'-GCIGCIIARIGGRTTICC-3'. Take loofah genomic DNA 50ng as a template, and ca...

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Abstract

The invention mainly relates to loofah gummy stem blight-resistant gene segments or gene markers and application thereof. Three loofah gummy stem blight-resistant gene segments or gene markers are rapidly separated by mainly adopting an anti-disease gene homologous sequencing method; and through sequencing, the lengths of nucleotides of these gene segments or gene markers are respectively 504bp, 504bp and 504bp. According to the loofah gummy stem blight-resistant gene segments or gene markers, the excavating period of a loofah gummy stem blight-resistant gene is effectively shortened; rapid identification of a gummy stem blight gene is facilitated; corresponding co-separation functional markers (SNP, SCAR and the like) are developed through identified gummy stem blight; the gene segments or gene markers can also be applied to assistant selection of molecular markers of the gummy stem blight-resistant gene; the accuracy is high; creation of multi-resistance breeding materials can be carried out by combining loofah gummy stem blight-resistant gene markers with molecular markers for other anti-disease genes; the breeding period is shortened; the breeding efficiency is improved; and a foundation is laid for elaboration of molecular mechanism of the loofah gummy stem blight-resistant gene.

Description

technical field [0001] The invention utilizes the homologous sequence method of the disease-resistant gene to clone loofah resistance gene fragments or transform them into molecular markers, etc., and mainly relates to conventional hybrid breeding of loofah, molecular marker-assisted breeding, positioning and molecular markers of resistance gene of loofah Mapping, the cloning of the resistance gene and the analysis of the regulation mechanism of the resistance gene belong to the field of plant biotechnology science. Background technique [0002] Blight is one of the most important diseases in loofah production. Damage stems, but also damage leaves and fruits. Stem lesions are oval to fusiform, with brown edges and taupe in the middle. Sometimes the affected part overflows with amber resinous jelly, and eventually the stems die. Leaf spots are nearly round, ranging from 10 to 20 mm in diameter, brown or dark brown, with slight ring patterns. The fruit is infected with nea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 戴新钱孝英钱春桃
Owner CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP
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