Quick identification of MLO powdery mildew resistant gene for citrus

A powdery mildew resistance gene and citrus technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problems of long time and difficult cloning, and achieve the goal of shortening the breeding period, shortening time, and low efficiency Effect

Inactive Publication Date: 2015-04-29
CHANGSHU CITY DONGBANG TOWN LIMU VEGETABLE PROFESSION COOP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the basic research of citrus is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

Method used

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  • Quick identification of MLO powdery mildew resistant gene for citrus
  • Quick identification of MLO powdery mildew resistant gene for citrus
  • Quick identification of MLO powdery mildew resistant gene for citrus

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Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the citrus powdery mildew gene. The specific implementation process is as follows:

[0032] 1) Collection and identification of citrus MLO-type genes

[0033] In order to obtain all the MLO gene family members of citrus, we first constructed an HMM model using the MLO gene sequences of Arabidopsis, tomato, pea, pepper, rose, pepper, and lotus root MLO gene sequences for resistance to powdery mildew. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC285032,TC290021,TC302...

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Abstract

The invention provides quick identification of a powdery mildew resistant gene for citrus, relates to subject knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The quick identification mainly comprises the following steps: (1) downloading a citrus whole-genome sequence and acquiring an MLO gene; (2) identifying the MLO gene; (3) establishing a phylogenetic relationship of the MLO gene; and (4) comparing an MLO powdery mildew gene. According to the quick identification, the mining period of the citrus powdery mildew gene is effectively shortened to facilitate quick identification of the powdery mildew gene; the corresponding coseparation functional markers (SNP, SCAR and the like) are developed through the identified candidate powdery mildew gene and quickly used for molecular marker auxiliary selection of the powdery mildew resistant gene, and the accuracy is high; by virtue of combination with other anti-disease gene molecular markers, creation of a multi-resistant breeding material can be carried out, the breeding period is shortened, and the breeding efficiency is improved; and a foundation is laid for stating the citrus powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the citrus powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies by means of the whole genome sequence of citrus, and mainly involves the download of the whole genome sequence of citrus and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Citrus belongs to the Rutaceae Citrus genus and is a small evergreen tree or shrub. Citrus fruit is rich in nutrition, excellent in color, fragrance and taste. It can be eaten fresh or processed into various processed products mainly fruit juice. The output of citrus ranks first in all kinds of fruits, and citrus juice accounts for 3 / 4 of fruit juice, which is widely favored by consumers. Citrus has the highest vitamin C content and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 钱春桃其他发明人请求不公开姓名
Owner CHANGSHU CITY DONGBANG TOWN LIMU VEGETABLE PROFESSION COOP
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