Quick identification of MLO powdery mildew resistant gene for citrus

Abstract

The invention provides quick identification of a powdery mildew resistant gene for citrus, relates to subject knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The quick identification mainly comprises the following steps: (1) downloading a citrus whole-genome sequence and acquiring an MLO gene; (2) identifying the MLO gene; (3) establishing a phylogenetic relationship of the MLO gene; and (4) comparing an MLO powdery mildew gene. According to the quick identification, the mining period of the citrus powdery mildew gene is effectively shortened to facilitate quick identification of the powdery mildew gene; the corresponding coseparation functional markers (SNP, SCAR and the like) are developed through the identified candidate powdery mildew gene and quickly used for molecular marker auxiliary selection of the powdery mildew resistant gene, and the accuracy is high; by virtue of combination with other anti-disease gene molecular markers, creation of a multi-resistant breeding material can be carried out, the breeding period is shortened, and the breeding efficiency is improved; and a foundation is laid for stating the citrus powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the citrus powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies by means of the whole genome sequence of citrus, and mainly involves the download of the whole genome sequence of citrus and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Citrus belongs to the Rutaceae Citrus genus and is a small evergreen tree or shrub. Citrus fruit is rich in nutrition, excellent in color, fragrance and taste. It can be eaten fresh or processed into various processed products mainly fruit juice. The output of citrus ranks first in all kinds of fruits, and citrus juice accounts for 3 / 4 of fruit juice, which is widely favored by consumers. Citrus has the highest vitamin C content and i...

AI Technical Summary

Problems solved by technology

However, because the basic research of citrus is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

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