The invention belongs to the technical field of
gene engineering, and particularly relates to application of LjPUB7
protein to regulation and control of
plant root nodule nitrogen fixation efficiency and / or regulation and control of
plant yield. According to the present invention, the LjPUB7
gene is knocked out through the
CRISPR-CAS9 technology, and the LjPUB7
mutant plant is obtained through the
lotus corniculatus stable transformation technology; after
inoculation of
rhizobium, compared with a
wild type, an infection line of an LjPUB7
gene mutant plant is remarkably improved, which indicates that the LjPUB7 gene regulates and controls development of root nodules; results of nodulation experiments show that after the LjPUB7 is knocked out, the number of root nodules and the density of the root nodules can be remarkably increased, and it shows that the LjPUB7 gene and the
protein expressed by the LjPUB7 gene regulate and control the formation of early-stage root nodules and the
nitrogen fixation efficiency of leguminous plants. By inhibiting the expression of the gene, the
nitrogen fixation efficiency of the leguminous plants can be improved, so that the
biomass and the yield of the plants are increased, and the gene has an application prospect in the research of a leguminous
crop nitrogen fixation mechanism and agricultural production.