An oil-control and skin-astringing composition, its preparation method and application

Abstract

The present invention discloses an oil-control and skin-astringing composition, its preparation method and application, specifically belonging to the field of toilet preparations. The oil-control and skin-astringing composition comprises: 1.5 - 2.1 parts of tea extract, 7.1 - 8.6 parts of witch hazel water, 0.003 - 0.01 part of moso bamboo leaf extract, 0.036 - 0.051 part of bird's-foot trefoil seed extract and 0.6 - 1.2 parts of ginkgo purple root extract. The oil-control and skin-astringing composition provided by the present invention has excellent performance in inhibiting the oil secretion of human sebaceous gland cells and has excellent deep oil-control effect on the skin; the leave-on skin topical agent provided by the present invention has excellent short-term oil-control, long-term oil-control and skin-astringing effects.

Description

Technical Field

[0001] The present invention belongs to the field of toilet preparations, and particularly relates to an oil-control and skin-astringing composition, a preparation method thereof, and an application thereof. Background Art

[0002] In the modern skin care field, oil control and pore shrinking are the focus of many consumers. Excessive sebum secretion and enlarged pores not only affect the appearance of the skin, but also easily cause skin problems such as acne and pimples. Therefore, it is of great significance to develop a skin care composition that can effectively control oil and shrink pores.

[0003] Witch hazel water contains various beneficial components such as vitamins, tannins, and flavonoids, and has the effects of anti-inflammatory and antioxidant, regulating sebum secretion, shrinking pores, and evening skin tone. Tea extracts have a wide range of applications in cosmetics. There are more than 1,400 substances in tea, including tea polyphenols, purine alkaloids, amino acids, proteins, vitamins, mineral elements, and tea saponins, and have significant antioxidant, anti-inflammatory and antibacterial, pore shrinking, anti-wrinkle and anti-aging effects. Although witch hazel water and tea extracts each have significant skin care effects, a single ingredient often fails to fully meet consumers' comprehensive requirements for oil control and pore shrinking.

[0004] Therefore, developing an oil-control and skin-astringing composition that organically combines multiple natural ingredients can not only give play to their respective advantages, but also promote the oil-control and skin-astringing effects between witch hazel water and tea extracts through synergistic effects, achieving a more significant skin care effect. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a composition with oil-control and skin-astringing effects containing multiple plant extracts such as tea extract, witch hazel water, moso bamboo leaf extract, lotus corniculatus seed extract, and ginkgo purple root extract, as well as a preparation method and an application thereof, and achieve a more significant oil control and pore shrinking effect through the organic combination of natural plant ingredients.

[0006] To achieve the above purpose, the present invention discloses the following technical solutions:

[0007] In the first aspect, the present invention provides an oil-control and skin-astringing composition, and by mass, the composition contains the following components:

[0008]

[0009]

[0010] Preferably, by mass, the composition contains the following components:

[0011]

[0012] Further preferably, by mass parts, the composition comprises the following components:

[0013]

[0014] Even more preferably, by mass parts, the composition comprises the following components:

[0015]

[0016] In a second aspect, the present invention provides the use of the aforementioned oil-control and skin-astringing composition in the preparation of a skin care product having the effects of oil control, acne treatment, antioxidant, antibacterial, anti-inflammatory and / or soothing.

[0017] In a third aspect, the present invention provides a leave-on topical skin agent having the effects of oil control and skin astringency, and the leave-on topical skin agent contains the aforementioned oil-control and skin-astringing composition;

[0018] The addition amount of the oil-control and skin-astringing composition in the leave-on topical skin agent is 5-10 wt%.

[0019] Preferably, the leave-on topical skin agent further comprises a thickener, a humectant, a preservative, a chelating agent, an emulsifier, a pH regulator and a solvent.

[0020] Further preferably, the thickener is selected from at least one of acrylate / C10-30 alkyl acrylate cross-linked polymer and carbomer;

[0021] The humectant is selected from at least one of glycerol, allantoin, 1,3-propanediol, butanediol and 1,2-hexanediol;

[0022] The preservative is p-hydroxyacetophenone;

[0023] The chelating agent is disodium EDTA;

[0024] The emulsifier is PPG-5-ceteth-20;

[0025] The pH regulator is arginine;

[0026] The solvent is deionized water.

[0027] In a fourth aspect, the present invention provides a preparation method of the leave-on topical skin agent described in the third aspect, and the preparation method includes the following steps:

[0028] S1. Stir and mix the thickener, the humectant and deionized water, heat to 80-90 °C, homogenize at a rotation speed of 1000-1500 rpm for 5-10 min, keep warm for standby after homogenization to obtain component A;

[0029] S2. Stir and mix the preservative, chelating agent, emulsifier and deionized water, heat to 50 - 60 °C, homogenize at a rotation speed of 800 - 1000 rpm for 3 - 5 min, keep warm for standby after homogenization to obtain Component B;

[0030] S3. Cool Component A to 50 - 60 °C, add Component B and stir and mix at a rotation speed of 500 - 600 rpm for 5 - 10 min, then cool to below 45 °C, add the oil-control and pore-tightening composition and stir and mix at a rotation speed of 500 - 600 rpm for 5 - 10 min, finally add a pH regulator to adjust the pH to 5.5 - 6.5, stop stirring, discharge to obtain a leave-on topical skin agent.

[0031] In the present invention:

[0032] The tea extract is rich in tea polyphenols, alkaloids, tea polysaccharides, theanine, vitamins, etc., has antioxidant effects, can scavenge free radicals, delay skin aging. The tea extract also has astringency, can shrink thick pores, reduce wrinkles, and at the same time has antibacterial and anti-inflammatory effects. In terms of oil control, the tea extract can inhibit sebum secretion, reduce oiliness, and help maintain the skin's water-oil balance.

[0033] Witch hazel water is rich in tannic acid, gallic acid, catechins, proanthocyanidins, flavonoids, choline, saponins, etc., and has the effects of pore-tightening, anti-inflammatory and calming, oil control and balance, and antioxidant. For oily and combination skin, witch hazel water can control the secretion of excess oil, reduce skin greasiness, and at the same time has a moisturizing effect. Witch hazel water can also reduce redness and inflammation and soothe sensitive skin.

[0034] The extract of Phyllostachys heterocycla var. pubescens leaves contains various flavonoid compounds, and has the effects of antioxidant, antibacterial and anti-inflammatory, regulating blood lipid and anti-cell aging. The extract of Phyllostachys heterocycla var. pubescens leaves can help control oil, and it can regulate sebum secretion and reduce skin greasiness.

[0035] The extract of Lotus corniculatus seeds has astringent and anti-inflammatory effects, helps reduce skin oil secretion, and at the same time provides soothing and moisturizing effects.

[0036] The extract of Prunus armeniaca L. var. ansu Maxim. and Lithospermum erythrorhizon Sieb. et Zucc. has antioxidant and anti-inflammatory effects, can reduce skin oil secretion, and at the same time has the effect of pore-tightening.

[0037] The present invention has found through research that when tea extract, witch hazel water, moso bamboo leaf extract, lotus corniculatus seed extract, and ginkgo purple root extract are used simultaneously, their effects can be enhanced. Specifically, both tea extract and witch hazel water have astringent and oil-control effects, and when used together, they can more effectively reduce sebum secretion and shrink pores; the antioxidant and anti-inflammatory properties of moso bamboo leaf extract and lotus corniculatus seed extract can bring effects such as soothing the skin, reducing inflammation and oil secretion; the addition of ginkgo purple root extract can further enhance the oil-control effect, and they promote each other to obtain a more excellent effect of controlling oil and astringing the skin.

[0038] Advantages of the present invention:

[0039] 1. The oil-control and skin-astringing composition provided by the present invention has excellent performance in inhibiting the oil secretion of human sebaceous gland cells and has an excellent deep oil-control effect on the skin;

[0040] 2. The leave-on skin topical agent provided by the present invention has excellent short-term oil-control, long-term oil-control, and skin-astringing effects. Compared with the blank control group, the test group has obvious effects in controlling oil and reducing pore coarsening. Specific embodiments

[0041] To make the objectives, technical solutions, and advantages of the present invention clearer and more understandable, the following further describes the present invention in detail with reference to specific embodiments. However, the following embodiments are only the preferred embodiments of the present invention and not all of them. For clarity, not all features of the actual embodiments are described.

[0042] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0043] In the present invention:

[0044] The tea extract is an extract of the leaves of Camellia sinensis, and its INCI name / English name is Camellia sinensis leaf extract, which is purchased from Yunnan Tangren Biotechnology Co., Ltd.;

[0045] The witch hazel water is the water of Hamamelis virginiana, and its INCI name / English name is Hamamelis virginiana (witch hazel) water, which is purchased from Ganzhou Huahan Biotechnology Co., Ltd.;

[0046] The Phyllostachys pubescens leaf extract is the extract of the leaves of Phyllostachys pubescens. Its INCI name / English name is PHYLLOSTACHYS PUBESCENS LEAF EXTRACT, and it is purchased from Golden Basin Biotechnology Co., Ltd.;

[0047] The Lotus corniculatus seed extract is the extract of the seeds of Lotus corniculatus. Its INCI name / English name is LOTUS CORNICULATUS SEED EXTRACT, and it is purchased from Shanghai Jiakai Biotechnology Co., Ltd.;

[0048] The Lithospermum officinale root extract is the extract of the roots of Lithospermum officinale. Its INCI name / English name is LITHOSPERMUM OFFICINALE ROOT EXTRACT, and it is purchased from Mufan Biotechnology;

[0049] Other raw materials are commercially available.

[0050] Preparation of the composition:

[0051] Accurately weigh the raw materials in Table 1, mix and stir evenly to obtain the oil-control and skin-astringing composition.

[0052] Table 1 Mass ratio of the composition raw materials

[0053]

[0054] Note: "-" in the table indicates no addition.

[0055] Sebum secretion inhibition test:

[0056] Test cells: The immortalized human sebaceous gland cell line SZ95 is selected. The cell line has passed the cell line quality control test and the results are qualified. The passage number for this test is P10.

[0057] Test consumables: Fetal bovine serum, high-glucose DMEM medium, penicillin, streptomycin, phosphate buffer (PBS), improved Oil Red detection kit, paraformaldehyde, isopropanol, isotretinoin, dihydrotestosterone (DHT).

[0058] Complete medium: Add 10% fetal bovine serum, 100 U / mL penicillin, and 100 μg / mL streptomycin to the high-glucose DMEM medium;

[0059] Test sample group: Complete medium containing the test sample and 1 μmol / L DHT. Among them, the test sample is Component 1-12, and the concentration of the test sample in the complete medium is 5 wt% and 10 wt%;

[0060] Blank control group: Complete medium;

[0061] Negative control group: Complete medium containing 1 μmol / L DHT;

[0062] Positive control group: Complete medium containing 1 μmol / L DHT and 5 μmol / L isotretinoin.

[0063] Experimental protocol:

[0064] (1) Cell preparation: Inoculate well - growing human sebaceous gland cells into the complete medium in a 24 - well plate, with 5×10 4 cells per well. Add 500 μL of PBS solution to the edge wells of the plate. Set up blank control group, negative control group, positive control group and test sample group, with three replicates in each group. Place the 24 - well plate in an incubator at 37°C and 5% CO 2 overnight.

[0065] (2) Drug administration: After overnight culture, change the complete medium in the 24 - well plate and carry out drug administration. The blank control group is given 1 mL of complete medium, the sample group is given 1 mL of complete medium containing the test sample at the corresponding concentration and 1 μmol / L DHT, the positive control group is given 1 mL of complete medium containing 1 μmol / L DHT and 5 μmol / L isotretinoin, and the negative control group is given 1 mL of complete medium containing 1 μmol / L DHT. After drug administration, place the 24 - well plate in an incubator at 37°C and 5% CO 2 for 48 h.

[0066] (3) Oil droplet detection: After incubation, wash with PBS, fix with 4% paraformaldehyde for 30 min, wash with distilled water for 2 min, wash twice. Detect according to the instruction manual of the oil red detection kit. Use an inverted microscope and an enzyme - linked immunosorbent assay (ELISA) reader to detect the oil droplet situation in each sample well, and take the average value of the three replicates for each group.

[0067] Judgment criteria:

[0068] Oil Red is a fat-soluble azo dye that can specifically stain neutral lipids such as triglycerides in cells or tissues, but has a weaker staining effect on lipids such as phospholipids and steroids. When cell or tissue sections are immersed in the Oil Red staining solution, Oil Red can dissolve in the fat (such as lipid droplets) in the cells or tissues, making them appear red or orange-red. The staining conditions in the tissues or cells can be observed through a microscope to compare the differences in triglyceride accumulation among different groups. After staining, lipids can be extracted with 60% isopropanol, and the absorbance of the dye at 510 nm can be measured for quantitative comparison. Under the same stimulation condition of 1 μmol / L DHT, compared with the negative control group, the content of oil droplets in the test sample group decreased, indicating that the sample has the ability to inhibit sebum secretion at the test concentration. The inhibition rate calculation formula is: Inhibition rate (%) = (Oil droplet content in the negative control group - Oil droplet content in the test sample group) / Oil droplet content in the negative control group × 100%. The results are shown in Table 2.

[0069] Table 2 Relative content of oil droplets and inhibition rate in sebaceous gland cells

[0070]

[0071]

[0072] Result analysis:

[0073] Compared with the negative control group, the content of oil droplets in Components 1-4 all showed a decrease. The sebum secretion inhibition rates of Components 1-4 with a concentration of 10 wt% in human sebaceous gland cells reached 30.11%, 31.26%, 33.15%, and 30.74% respectively, indicating that the oil-control and skin-astringing composition provided by the present invention has excellent performance in inhibiting sebum secretion in human sebaceous gland cells and has excellent deep oil-control efficacy on the skin.

[0074] Preparation of the examples:

[0075] Weigh each raw material according to Table 3;

[0076] S1. Stir and mix the thickener, humectant and deionized water, heat to 90 °C, homogenize at a rotation speed of 1200 rpm for 10 min, and keep warm for standby after homogenization to obtain Component A;

[0077] S2. Stir and mix the preservative, chelating agent, emulsifier and deionized water, heat to 60 °C, homogenize at a rotation speed of 800 rpm for 5 min, and keep warm for standby after homogenization to obtain Component B;

[0078] S3. Cool down component A to 60°C, add component B and stir for 10 min at a rotation speed of 500 rpm, then cool down to below 45°C, add the oil-control and skin-astringing composition and stir for 10 min at a rotation speed of 500 rpm. Finally, add a pH regulator to adjust the pH to 6, stop stirring, discharge the mixture, and obtain the essence.

[0079] Table 3 Mass percentages of raw materials in the examples

[0080]

[0081] Note: "-" in the table indicates no addition.

[0082] Performance test of the examples:

[0083] 1 Oil-control effect test

[0084] 1.1 Instant oil-control effect test

[0085] Take Examples 1-3 above as the test groups and the blank control example as the blank control group, and conduct an instant oil-control effect test on the arm skin of skin subjects.

[0086] Test method: Select 15 male subjects, divide them into 3 groups evenly with 5 subjects in each group. Select two 4 cm × 4 cm test areas at corresponding positions on the inner sides of the two arms of the 15 skin subjects, and mark them as A and B respectively. Use latex finger cots to apply (1.0 ± 0.1) mg / cm 2 squalane in the two areas. After 3 minutes of application, apply (2.0 ± 0.1) mg / cm 2 of the blank control example as the blank control in area A, and apply (2.0 ± 0.1) mg / cm 2 of the test sample of the test group in area B. After applying the test sample, use the sebum test system of the SkinLab Combo skin detector provided by Cortex, Denmark. Use the instrument probe to test the test area and give the oiliness value. Take the average value (retained to one decimal place) of the measured results of all subjects as the final result of this test sample. The oiliness value data of each group are shown in Table 4.

[0087] Table 4 Test results of oiliness values

[0088]

[0089]

[0090] 1.2 Long-lasting oil-control effect test

[0091] The above-prepared Examples 1-3 and the blank control example were used as test samples, wherein Examples 1-3 were used as the test group, and the blank control example was used as the blank control group, and the forehead skin of the skin test subjects was tested for the long-lasting oil control effect. The sebum test system of the SkinLab Combo skin detector provided by the Danish Cortex company was still used to test the oil content.

[0092] Test method: 20 male subjects were selected. There was no significant difference in the facial oil level of these 20 subjects. They were divided into 4 groups, with 5 people in each group. The method of use was that the subjects took 2.0±0.5g of the test sample and evenly applied it on the face in the form of smear after washing their faces with the standard cleanser in the morning and evening; the subjects performed a facial oil content test before starting to use the test sample, and the initial value was recorded as W0; the facial oil content test was performed after using the test sample for 2 weeks, recorded as W2; the facial oil content test was performed after using the test sample for 4 weeks, recorded as W4; the average value of each group of data was taken (keeping two decimal places), and the specific test results are shown in Table 5.

[0093] Table 5 Grease content test results

[0094]

[0095] 1.3 Facial pore change detection

[0096] The above-prepared Examples 1-3 and the blank control example were used as test samples, wherein Examples 1-3 were used as the test group, and the blank control example was used as the blank control group, and the facial skin of the skin test subjects was subjected to pore feature counting detection. The facial image acquisition device VISIA provided by Canfield Company of the United States was used to detect the pore feature counting. The pore feature count is the number of pores obtained by VISIA statistics, and the parameter is obtained by VISIA's own software analysis.

[0097] Experimental method: 20 male subjects were selected. There was no significant difference in the facial pore feature count levels of these 20 subjects. They were divided into 4 groups, with 5 people in each group. The method of use was that the subjects took 2.0±0.5g of the test sample and evenly applied it on the face in the form of smear after washing their faces with the standard facial cleanser in the morning and evening; the subjects performed a pore feature count test before starting to use the test sample, recorded as W0; the pore feature count test was performed after using the test sample for 2 weeks, recorded as W2; the pore feature count test was performed after using the test sample for 4 weeks, recorded as W4; the average value of each group of data was taken (keeping one decimal place), and the specific results are shown in Table 6 below.

[0098] Table 6 Changes in pore feature counts

[0099]

[0100] 1.4 Result Analysis

[0101] According to the test results, it can be seen that the skin topical agent for residence provided by the present invention has excellent short-term oil control, long-term oil control and skin astringency effects. Among them, compared with the blank control group, the test group has obvious effects of oil control and reduction of enlarged pores. At the same time, the oil control and skin astringency performance of the skin topical agent increases with the increase of the concentration of the composition, showing concentration dependence, indicating that the composition is a key ingredient for oil control and skin astringency in the overall formulation.

[0102] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that this is only an example, and the protection scope of the present invention is defined by the appended claims. Without departing from the principles and essence of the present invention, those skilled in the art can make various changes or modifications to these embodiments, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. An oil-controlling and skin-smoothing composition, characterized in that: The composition consists of the following components in parts by mass: 1.5-2.1 parts of tea extract; 7.1-8.6 parts of witch hazel water; 0.03-0.1 parts of moso bamboo leaf extract; Lotus japonicus seed extract 0.36-0.51 parts; 0.6-1.2 parts of Ginkgo biloba root extract.

2. The oil-controlling skin-smoothing composition according to claim 1, characterized in that: The composition consists of the following components in parts by mass: 1.7-2.1 parts of tea extract; 7.6-8.6 parts of witch hazel water; 0.06-0.1 parts of moso bamboo leaf extract; Lotus japonicus seed extract 0.41-0.51 parts; 0.8-1.2 parts of Ginkgo biloba root extract.

3. The oil-controlling skin-smoothing composition according to claim 2, characterized in that: The composition consists of the following components in parts by mass: 1.9-2.1 parts of tea extract; 8.1-8.6 parts of witch hazel water; 0.08-0.1 parts of moso bamboo leaf extract; Lotus japonicus seed extract 0.46-0.51 parts; 1.0-1.2 parts of Ginkgo biloba root extract.

4. The oil-controlling skin-smoothing composition according to claim 3, characterized in that: The composition consists of the following components in parts by mass: 1.9 parts of tea extract; 8.1 parts of witch hazel water; 0.08 parts of moso bamboo leaf extract; Lotus japonicus seed extract 0.46 parts; 1.0 parts of Ginkgo biloba root extract.

5. Use of the oil-controlling and skin-smoothing composition according to any one of claims 1 to 4 in preparing a skin care product with oil-controlling effect.

6. A leave-on skin topical preparation with oil-controlling and skin-smoothing effects, characterized in that: The leave-on skin topical preparation comprises the oil-controlling and skin-smoothing composition according to any one of claims 1 to 4; The amount of the oil-controlling and skin-astringing composition added to the leave-on skin external preparation is 5-10 wt %.

7. The leave-on skin external preparation according to claim 6, characterized in that: The leave-on skin external preparation further comprises a thickener, a moisturizer, a preservative, a chelating agent, an emulsifier, a pH adjuster, and a solvent.

8. The leave-on skin external preparation according to claim 7, characterized in that: The thickener is selected from at least one of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer and carbomer; The moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol; The preservative is p-hydroxyacetophenone; The chelating agent is disodium EDTA; The emulsifier is PPG-5-cetoeth-20; The pH regulator is arginine; The solvent is deionized water.

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