Glucuronic acid transferase, gene encoding same, and method for using same

By isolating the gene of the first transferase of glucuronic acid that can catalyze soybean soybean alcohol B from soybean and expressing the enzyme in the biological expression system, the problem of difficulty in obtaining glycyrrhizone stably and continuously in the prior art is solved, and the effect of efficient biosynthesis of glycyrrhizone in the biological system is achieved.

CN119685182APending Publication Date: 2025-03-25OSAKA UNIVERSITY +1
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Patent Information

Application Number
CN202411891074.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2019-10-17
Filing Date
2020-10-16
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

The prior art is difficult to obtain sufficient amounts of glycyrrhizin in a stable and continuous manner because there is a lack of glucuronic acid first transferase that catalyzes the direct transfer of glucuronic acid.

Method used

By isolating the cellulose synthase-like gene that can catalyze glucuronic acid transfer of the 3-position hydroxyl group of soybean soybean soybean B, the glucuronic acid first transferase was successfully identified, and a large number of biosynthetic glycyrrhizones were used to biosynthesis in the biological expression system.

Benefits of technology

The stable and continuous biosynthesis of licorice saponins in the biological expression system is achieved, and the problem of difficulty in obtaining sufficient amount of licorice saponins in the prior art is solved.

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Abstract

A gene of a glucuronic acid first transferase that catalyzes glucuronic acid transfer to a hydroxyl group at position 3 of an oleanane-type triterpenoid is identified. Provided is a glucuronic acid first transferase gene which is derived from leguminous plants (Soybean, Liquorice, and Japanese Lotus corniculatus), comprises a base sequence represented by SEQ ID NO: 2, SEQ ID NO: 4, and SEQ ID NO: 6, and has a desired activity.
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Citation Information

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