Method for rapidly identifying orange MLO powdery mildew resistance gene

A powdery mildew resistance gene and powdery mildew resistance technology, which is applied in genetic engineering, plant genetic improvement, angiosperm/flowering plants, etc., can solve the problems of long time and difficult cloning, and achieve shortening breeding years, shortening time and efficiency low effect

Inactive Publication Date: 2012-10-10
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research on sweet orange is not deep enough, it is not only ti

Method used

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  • Method for rapidly identifying orange MLO powdery mildew resistance gene
  • Method for rapidly identifying orange MLO powdery mildew resistance gene
  • Method for rapidly identifying orange MLO powdery mildew resistance gene

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Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the sweet orange powdery mildew gene. The specific implementation process is as follows:

[0032] 1) Collection and identification of sweet orange MLO gene

[0033] In order to obtain all MLO-type gene family members of sweet orange, we first constructed an HMM model based on the MLO-type genes of Arabidopsis thaliana, and the MLO gene sequences of resistance to powdery mildew of tomato, pea, pepper, rose, pepper, and japonicus japonicus. Search for MLO-type genes in the genome sequence; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC,315947 TC325903, TC315944, TC315912, TC322759, TC322059, TC330654, ...

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Abstract

The present invention is a method for rapidly identifying an orange MLO powdery mildew resistance gene, relating to plant comparative genomics, genetics, bioinformatics and other disciplines of knowledge, and belonging to the field of plant biotechnology science. The method in the invention mainly comprises the following steps of: 1) download of an orange full genome sequence and collection of an MLO gene; 2) identification of the MLO gene; 3) identification of a candidate MLO powdery mildew gene through phylogenetic relationship of the MLO gene; and 4) comparison of the MLO powdery mildew gene. The method in the invention can effectively shorten a mining cycle of the orange powdery mildew gene, facilitating rapid identification of the powdery mildew gene; the method can develop corresponding cosegregation functional markers (SNP, SCAR and the like) through the identified candidate powdery mildew gene and can also be used in fast molecular marker-assisted selection of the powdery mildew resistance gene with high accuracy; the method can also create multiple breeding materials with resistance property through combination of other resistance gene molecular markers, shortening a breeding period and improving breeding efficiency; the method can further lays a foundation for describing an orange powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the powdery mildew gene of sweet orange by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategy by means of the whole genome sequence of sweet orange, and mainly involves the download of the whole genome sequence of sweet orange, candidate Gene identification, gene comparison, clustering and other means to identify the powdery mildew gene belong to the field of plant biotechnology science. Background technique [0002] Sweet orange is a fruit tree of the genus Citrus (Citurs) in the citrus subfamily of Rutaceae (Rutaceae), also known as yellow fruit, orange, golden ball, golden orange, etc. , my country is one of the countries of origin of sweet oranges, and the main producing areas are mainly in Sichuan, Guangdong, Taiwan, Guangxi, Fujian, Hunan, Jiangxi, Hubei and other provinces. In the world's citrus production, sweet orange accounts for the largest propo...

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12Q1/68A01H5/00
Inventor 钱孝英袁伟
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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