Method for rapidly identifying orange MLO powdery mildew resistance gene

Abstract

The present invention is a method for rapidly identifying an orange MLO powdery mildew resistance gene, relating to plant comparative genomics, genetics, bioinformatics and other disciplines of knowledge, and belonging to the field of plant biotechnology science. The method in the invention mainly comprises the following steps of: 1) download of an orange full genome sequence and collection of an MLO gene; 2) identification of the MLO gene; 3) identification of a candidate MLO powdery mildew gene through phylogenetic relationship of the MLO gene; and 4) comparison of the MLO powdery mildew gene. The method in the invention can effectively shorten a mining cycle of the orange powdery mildew gene, facilitating rapid identification of the powdery mildew gene; the method can develop corresponding cosegregation functional markers (SNP, SCAR and the like) through the identified candidate powdery mildew gene and can also be used in fast molecular marker-assisted selection of the powdery mildew resistance gene with high accuracy; the method can also create multiple breeding materials with resistance property through combination of other resistance gene molecular markers, shortening a breeding period and improving breeding efficiency; the method can further lays a foundation for describing an orange powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the powdery mildew gene of sweet orange by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategy by means of the whole genome sequence of sweet orange, and mainly involves the download of the whole genome sequence of sweet orange, candidate Gene identification, gene comparison, clustering and other means to identify the powdery mildew gene belong to the field of plant biotechnology science. Background technique [0002] Sweet orange is a fruit tree of the genus Citrus (Citurs) in the citrus subfamily of Rutaceae (Rutaceae), also known as yellow fruit, orange, golden ball, golden orange, etc. , my country is one of the countries of origin of sweet oranges, and the main producing areas are mainly in Sichuan, Guangdong, Taiwan, Guangxi, Fujian, Hunan, Jiangxi, Hubei and other provinces. In the world's citrus production, sweet orange accounts for the largest propo...

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Problems solved by technology

However, because the basic research on sweet orange is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

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