Application of comparative genomics to rapid identification of phaseolus vulgaris mildew resistance locus o gene

Abstract

Relating to plant comparative genomics, genetics, bioinformatics and other disciplinary knowledge, the invention belongs to the scientific field of plant biotechnology, and discloses rapid identification of phaseolus vulgaris mildew resistance locus o gene. The main steps include: 1) download of phaseolus vulgaris whole genome sequence and acquisition of MLO type gene; 2) identification of MLO type gene; 3) MLO type gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew. The invention effectively shortens the mining cycle of phaseolus vulgaris powdery mildew gene, and is conducive to rapid identification of powdery mildew gene. Corresponding coseparation functional markers (SNP, SCAR and the like) are developed through identified candidate powdery mildew genes, which also can be used for molecular marker-assisted selection of mildew resistance locus o gene and have high accuracy. Multi-resistance breeding materials can be created by combining other resistant gene molecular markers, the breeding period can be shortened, and the breeding efficiency is improved, thus laying the foundation for elaborating the phaseolus vulgaris mildew resistance molecular mechanism.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies to quickly identify the powdery mildew gene of kidney bean by means of sequencing the whole genome sequence of kidney bean, and mainly involves the download of the whole genome sequence of kidney bean and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Kidney beans are the main edible bean crops for human beings, accounting for 50% of the total global edible bean production, and are one of the main sources of plant protein. Bean powdery mildew occurs widely in various parts of my country and is the main disease in production. The annual yield loss is as high as 30%, and even as high as 80% in serious cases. At present, in production, spraying fungicides and prom...

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Problems solved by technology

However, because the basic research of kidney bean is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

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