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Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon

A powdery mildew resistance gene and a technology for Brachypodium biscarp, which is applied in the field of plant biotechnology and science, can solve the problems of long time, difficulty in cloning, insufficient basic research on Brachypodium bismuth, and the like, so as to shorten the breeding period and shorten the time, the effect of improving breeding efficiency

Inactive Publication Date: 2012-10-03
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research of Brachypodium distachyon is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon
  • Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon
  • Rapid identification of anti-powdery-mildew gene of Brachypodium distachyon

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Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the powdery mildew gene of Brachypodium distachyon. The specific implementation process is as follows:

[0032] 1) Collection and identification of Brachypodium distachyon MLO-type genes

[0033] In order to obtain all the MLO-type gene family members of Brachypodium distachyon, we first constructed an HMM model based on the MLO-type genes of Arabidopsis thaliana, and the powdery mildew resistance MLO gene sequences of tomato, pea, pepper, rose, pepper, and japonicus. The MLO-type genes were retrieved from the Brachypodium distachyon genome sequence; secondly, the published MLO gene sequences in different crops were used as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, T...

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Abstract

The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.

Description

technical field [0001] The present invention uses methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies to quickly identify the Brachypodium distachyon powdery mildew gene by means of sequencing the whole genome sequence of Brachypodium distachyon; the invention mainly relates to two The downloading of the whole genome sequence of Brachypodium paniculata, the identification of candidate genes, the comparison of genes, clustering and other means, and then the identification of powdery mildew genes belong to the field of plant biotechnology science. Background technique [0002] Brachypodium distachybes ( Brachypodium distachyon ) is a temperate grass plant with relatively simple growth conditions, short plants, self-pollination, short growth cycle, strong reproductive ability, easy transformation, small genome, and rich genetic resources. Studies have shown that Brachypodium distachyon It has a common ancestor with wheat and rice,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68
Inventor 钱孝英
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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