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Molecular cloning and application of pork quality character related gene Myo6 (Myosin 6)

A technology of meat quality traits and genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of few research reports on pig Myo6 gene

Inactive Publication Date: 2015-12-02
HUNAN AGRICULTURAL UNIV
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  • Description
  • Claims
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Problems solved by technology

However, there are few research reports on the pig Myo6 gene

Method used

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  • Molecular cloning and application of pork quality character related gene Myo6 (Myosin 6)
  • Molecular cloning and application of pork quality character related gene Myo6 (Myosin 6)
  • Molecular cloning and application of pork quality character related gene Myo6 (Myosin 6)

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Embodiment Construction

[0034] The present invention will be further explained below in conjunction with specific embodiments, but the specific implementation does not limit the present invention in any way.

[0035] Take human Myo6 (GenBank accession number NC_000006.12) as the seed sequence, use BLASTN in GenBank, design specific primers with reference to pig EST (Expressed SequenceTags) whose homology is more than 80%, and clone the pig Myo6 gene using RACE technology cDNA full length. The Myo6 gene polymorphism is based on the use of comparative genomics methods to design primers based on the human Myo6 gene sequence, and use pig genomic DNA as a template to amplify. The amplified fragments are sequenced to screen SNPs, and it is found that there is a G / A base at the 709th place. Base mutations lead to PCR-RFLP-XbaI polymorphism, and use this to detect the distribution of the marker in domestic and foreign pig breeds.

[0036] Take 25-day-old Shaziling pig longissimus dorsi muscle, extract total RNA ...

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Abstract

The invention belongs to the technical field of livestock molecular biology, and particularly relates to molecular cloning and application of pork quality character related gene Myo6 (Myosin 6). The molecular marker disclosed by the invention is obtained through cloning the Myo6 gene, and the sequence of the Myo6 molecular marker is described as SEQ ID NO: 1. The polymorphism of the Myo6 gene is as follows: a primer is designed by using the comparative genomics method according to the My06 gene sequence of human, the genome DNA of pigs is adopted as a template for amplification, SNP is screened by sequencing the amplified fragments, genotyping is carried out by using PCR-RFLP, a G / A base mutation at 709bp is discovered, and PCR-RFLP-Xba I polymorphism is caused. The molecular cloning and application of pork quality character related gene Myo6 provide a novel molecular marker for the assistant selection of pig markers.

Description

Technical field [0001] The present invention belongs to the technical field of livestock molecular biology, and relates to a nucleic acid molecule containing pig gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to a method for molecular cloning of the Myo6 gene shown in SEQ ID NO:1, a single nucleotide polymorphism site in the polynucleotide sequence, and a method for detecting the single nucleotide polymorphism site. Background technique [0002] Pork is the main source of animal protein. As people's demand for meat increases, the requirements for meat quality increase accordingly. The factors affecting pork quality are genetic and non-hereditary. The intramuscular fat content of pigs has a great influence on meat quality traits. Generally speaking, the higher the intramuscular fat content, the tenderer the pork quality, and thus the better the meat quality. Meat quality traits are mostly quantitative traits, controlled by minor-effect polygenes. Ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/11C12Q1/68
Inventor 马海明柴玉兰王玲玉
Owner HUNAN AGRICULTURAL UNIV
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