Cloning and application of pork quality character related GADD45G gene molecule marker

A molecular marker-assisted, trait-based technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2015-11-18
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0006] At present, there are very few studies on pig GADD45G gene at home and abroad, and the study of molecular genetic markers of this gen

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  • Cloning and application of pork quality character related GADD45G gene molecule marker
  • Cloning and application of pork quality character related GADD45G gene molecule marker
  • Cloning and application of pork quality character related GADD45G gene molecule marker

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Embodiment Construction

[0027] The present invention will be further explained below in conjunction with specific examples, but the specific implementation does not limit the present invention in any way.

[0028] Primer design: use the method of comparative genomics to use the human GADD45G gene (GenBank accession number NM_003394.3) as the seed sequence, perform BLAST screening in GenBank for pig EST sequences with more than 80% homology, and screen out after sequence alignment GADD45G gene candidate SNPs site, select the GADD45G gene candidate SNPs site to design primers, and use the primers to amplify the gene DNA extracted from pig ear tissue.

[0029] Primer sequence: the forward primer is 5'-AAACTTGCTGCTTGCG-3' (SEQ ID NO: 2);

[0030]The reverse primer was 5'-GGATGGAGGCGACACT-3' (SEQ ID NO: 3).

[0031] DNA extraction: The present invention adopts the traditional phenol-chloroform-isoamyl alcohol method to extract 5 different pig breeds (325 large white pigs, 107 Taoyuan black pigs, 57 Shazi...

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Abstract

The invention belongs to the technical field of livestock molecular marker biology and particularly relates to cloning and application of a pork quality character related GADD45G gene molecule marker. The molecule marker provided by the invention is obtained by cloning a GADD45G gene and the sequence of the GADD45G molecule marker is shown in SEQ ID No.1. For polymorphism of the GADD45G gene, a primer is designed by using a comparative genomic method according to the GADD45G gene sequence of a human body, the genome DNA of a pig is used as a template for amplification, in an amplified fragment, sequencing is used for screening SNP, PCR-RFLP is used for genetic typing, that A/G base mutation exists at 640th bp in the SEQ ID No.1 is found, PCR-RFLP-PstI polymorphism is caused and the molecule marker can be used for association analysis of pork quality characters. According to the invention, the novel molecular marker is provided for pork quality character marker assistant selection.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular biology, and relates to a nucleic acid molecule comprising porcine gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to the molecular marker cloning method of the GADD45G gene shown in SEQ ID NO: 1, the single nucleotide polymorphism site in the polynucleotide sequence and its detection method. Background technique [0002] Pork is the most important meat on the table of nearly 1.4 billion people in my country. In 2014, the domestic pork production in China was 56.71 million tons, and the number of live pigs sold reached 735.1 million. Compared with local breeds in China, the growth rate of foreign pigs is very fast, but the poor meat quality is also an indisputable fact. The problem of pork quality has been widely concerned. Among many influencing factors, intramuscular fat content (IMF) is an important factor. IMF is the main index to evaluate the quality ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/11C12Q1/68
Inventor 马海明肖定福杨攀肖芝萍
Owner HUNAN AGRICULTURAL UNIV
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