Clone and application of pig meat quality character relevance WNT10B gene molecular mark

Abstract

The invention belongs to the field of the livestock molecule molecular biological technique and particularly relates to a clone and the application of a pig meat quality character relevance WNT10B gene molecular mark. The molecular mark is obtained by cloning pig WNT10B genes, and the sequence of the WNT10B molecular mark of the WNT10B gene is shown in SEQ ID NO:1. According to the polymorphism of the WNT10B, a primer is designed according to the WNT10B gene sequence of people with a genomics method, the genomic DNA of pigs is used as the template for amplification, SNP is screened by amplified fragments through sequencing, PCR-RFLP is used for conducting gene typing, a basic group mutation is found at the 552 bp position, polymorphism of PCR-RFLP-Sal I is caused, a new molecular mark is provided for auxiliary selection of the pig meat quality character mark, and the mark can be used for detecting the conditions of domestic and foreign pig breeds.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular biology, and relates to a nucleic acid molecule comprising a gene nucleotide related to pork quality traits as shown in SEQ ID NO:1. The present invention also relates to the molecular cloning method of the WNTOB gene shown in SEQ ID NO: 1, the single nucleotide polymorphism site in the polynucleotide sequence and its application in pig molecular marker-assisted selection. Background technique [0002] Pork is the most important meat on the table of 1.3 billion people in my country. In 2009, the number of pigs slaughtered in my country was 645 million, ranking first in the world. The growth rate of foreign breeds of pigs has reached a very high level, but it is an indisputable fact that the meat quality is poor. The problem of pork quality has attracted widespread attention. Among many influencing factors, intramuscular fat content (IMF) is an important influencing factor , IMF is the...

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Problems solved by technology

[0010] At present, there are few reports on the pig WNT10B gene and its binding protein and the differences in the expression of the gene in different tissues and different breeds of pigs at home and abroad. The bioinformatics analysis of the gene and the study of gene expression profiles will contribute to the molecular inheritance of the WNT10B gene. The basic basis for the study of gene functions such as characteristics and fat deposition in the muscle

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