Rapid Identification of pear powdery mildew resistance gene

Abstract

The invention relates to rapid identification of a pear powdery mildew resistance gene, and relates to the subject knowledge of plant comparative genomics, genetics and bioinformatics, belonging to the field of science of biotechnology. The rapid identification of the pear powdery mildew resistance gene comprises the following main steps of: 1) downloading of a complete genome sequence of a pear and collection of an MLO gene; 2) identification of the MLO gene; 3) MLO gene phylogenetic relationship; and 4) comparison of an MLO powdery mildew gene. The rapid identification of the pear powdery mildew resistance gene, provided by the invention, has the advantages of effectively shortening the pear powdery mildew gene mining period, and being favorable for the rapid identification of the powdery mildew gene; corresponding co-segregation functional markers (SNP, SCAR and the like) can be developed through the identification of candidate powdery mildew genes, and the co-segregation functional markers can be rapidly used for the molecular marker-assisted selection of the powdery mildew resistance gene, so that the accuracy is high; by combining other powdery mildew resistance gene molecular markers, a multi-resistance breeding material can be created, the breeding period can be shortened, and the breeding efficiency can be improved; a foundation is laid for explaining a pear powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the powdery mildew gene of pear by means of sequencing the whole genome sequence of pear, using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies, and mainly involves the download of the whole genome sequence of pear and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Pear belongs to Rosaceae Pyrus, and my country is one of the cradles of Pyrus plant center. The pear fruit is delicious, the meat is crisp and juicy, sweet and sour, and the flavor is fragrant and beautiful. Rich in sugar, protein, fat, carbohydrates and multivitamins, it plays an important role in human health. Pear fruit can also be processed to make dried pears, preserved pears, pear paste, pear juice, canned pears, etc., and...

AI Technical Summary

Problems solved by technology

Because the basic research of pears is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

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