Rapid Identification of pear powdery mildew resistance gene

A powdery mildew resistant gene and gene technology, applied in the field of plant biotechnology and science, can solve the problems of difficulty in cloning and long time, and achieve the effects of shortening the breeding period, shortening the time and improving the breeding efficiency.

Inactive Publication Date: 2015-04-29
CHANGSHU CITY DONGBANG DONGDUN VEGETABLE PROFESSION COOP
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the basic research of pears is not deep enough, it is not only time-co

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid Identification of pear powdery mildew resistance gene
  • Rapid Identification of pear powdery mildew resistance gene
  • Rapid Identification of pear powdery mildew resistance gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify pear powdery mildew gene. The specific implementation process is as follows:

[0031] 1) Collection and identification of pear MLO gene

[0032] In order to obtain all the members of the MLO gene family in pear, we first constructed an HMM model using the MLO gene sequences of Arabidopsis, tomato, pea, pepper, rose, pepper, and lotus root MLO gene sequences for resistance to powdery mildew. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC285032,TC290021,TC302716,TC283...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to rapid identification of a pear powdery mildew resistance gene, and relates to the subject knowledge of plant comparative genomics, genetics and bioinformatics, belonging to the field of science of biotechnology. The rapid identification of the pear powdery mildew resistance gene comprises the following main steps of: 1) downloading of a complete genome sequence of a pear and collection of an MLO gene; 2) identification of the MLO gene; 3) MLO gene phylogenetic relationship; and 4) comparison of an MLO powdery mildew gene. The rapid identification of the pear powdery mildew resistance gene, provided by the invention, has the advantages of effectively shortening the pear powdery mildew gene mining period, and being favorable for the rapid identification of the powdery mildew gene; corresponding co-segregation functional markers (SNP, SCAR and the like) can be developed through the identification of candidate powdery mildew genes, and the co-segregation functional markers can be rapidly used for the molecular marker-assisted selection of the powdery mildew resistance gene, so that the accuracy is high; by combining other powdery mildew resistance gene molecular markers, a multi-resistance breeding material can be created, the breeding period can be shortened, and the breeding efficiency can be improved; a foundation is laid for explaining a pear powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the powdery mildew gene of pear by means of sequencing the whole genome sequence of pear, using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies, and mainly involves the download of the whole genome sequence of pear and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Pear belongs to Rosaceae Pyrus, and my country is one of the cradles of Pyrus plant center. The pear fruit is delicious, the meat is crisp and juicy, sweet and sour, and the flavor is fragrant and beautiful. Rich in sugar, protein, fat, carbohydrates and multivitamins, it plays an important role in human health. Pear fruit can also be processed to make dried pears, preserved pears, pear paste, pear juice, canned pears, etc., and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12Q1/68
Inventor 钱春桃其他发明人请求不公开姓名
Owner CHANGSHU CITY DONGBANG DONGDUN VEGETABLE PROFESSION COOP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap