Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l
A powdery mildew resistance gene and powdery mildew resistance technology, applied in the field of rapid identification of papaya powdery mildew resistance gene by applying bioinformatics, can solve the problems of difficult cloning and long time, so as to shorten the breeding period, shorten the time, and improve the identification efficiency effect
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[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify papaya powdery mildew gene. The specific implementation process is as follows:
[0031] 1) Collection and identification of papaya MLO gene
[0032] In order to obtain all the members of the MLO gene family of papaya, we first constructed an HMM model using the MLO gene sequences of Arabidopsis thaliana and the MLO gene sequences of resistance to powdery mildew of tomato, pea, rose, pepper, and japonicus. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC3159259, 3 TC TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC293173, TC281861, TC283253, TC283383, TC285032, T...
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