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Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l

A powdery mildew resistance gene and powdery mildew resistance technology, applied in the field of rapid identification of papaya powdery mildew resistance gene by applying bioinformatics, can solve the problems of difficult cloning and long time, so as to shorten the breeding period, shorten the time, and improve the identification efficiency effect

Inactive Publication Date: 2012-10-10
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research of papaya is not deep enough, it is not only time-consuming to use these methods but also difficult to accurately clone these genes

Method used

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  • Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l
  • Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l
  • Application of bioinformatics in fast identifying powdery mildew resistance gene of carica papaya l

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Embodiment Construction

[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify papaya powdery mildew gene. The specific implementation process is as follows:

[0031] 1) Collection and identification of papaya MLO gene

[0032] In order to obtain all the members of the MLO gene family of papaya, we first constructed an HMM model using the MLO gene sequences of Arabidopsis thaliana and the MLO gene sequences of resistance to powdery mildew of tomato, pea, rose, pepper, and japonicus. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC3159259, 3 TC TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC293173, TC281861, TC283253, TC283383, TC285032, T...

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Abstract

The invention relates to fast identification of a powdery mildew resistance gene of carica papaya l, relating to subject knowledge including plant comparative genomics, genetics, bioinformatics and the like, belonging to the field of plant biotechnology sciences. Application of the invention mainly comprises the steps: 1) downloading complete genome sequence of the carica papaya l and acquiring MLO-type genes; 2) identifying the MLO-type genes; 3) researching phylogenetic relationships of the MLO-type genes; and 4) comparing MLO-type powdery mildew resistance genes. The invention has the advantages that discovery period of the powdery mildew resistance gene of the carica papaya l is effectively shortened, which assists in fast identification of the powdery mildew resistance gene; that identified candidate powdery mildew resistance genes are developed with corresponding co-segregation functional markers (SNP, SCAR, etc.), which can be used in fast auxiliary selection of molecular markers of the powdery mildew resistance gene with high accuracy; that other molecular markers of resistance genes can be combined to formulate multi-resistance breeding materials, which shortens breeding years and improves breeding yields; and that a foundation is laid to illustrate the powdery mildew resistance gene of the carica papaya l.

Description

technical field [0001] The present invention uses papaya sequencing whole genome sequence to quickly identify papaya powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies, and mainly involves the downloading of papaya whole genome sequence, candidate Gene identification, gene comparison, clustering and other means to identify the powdery mildew gene belong to the field of plant biotechnology science. Background technique [0002] papaya( Carica papaya L. ), also known as longevity fruit and milk melon, belongs to Papaya family, herbaceous dicotyledons, and is the preferred fruit tree in tropical and subtropical regions. With the continuous deepening of people's understanding of the nutritional and health value of papaya, its market demand is increasing. In recent years, powdery mildew has seriously harmed papaya planting areas, such as Yunnan and Guizhou, leading to early defoliation, greatly reduce...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68
Inventor 钱孝英俞锞
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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