Rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of melon

A powdery mildew resistant gene and melon technology, which is applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problems of physiological race variation of pathogens, environmental pollution, etc., and achieve shortened breeding years, low efficiency, and improved The effect of breeding efficiency

Inactive Publication Date: 2015-04-29
南农大(常熟)新农村发展研究院有限公司
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Problems solved by technology

At present, in production, the prevention and treatment of melon powdery mildew mainly depends on spraying fungicides, but long-term use of drugs will lead

Method used

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  • Rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of melon
  • Rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of melon
  • Rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of melon

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Embodiment Construction

[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the powdery mildew gene of melon. The specific implementation process is as follows:

[0031] 1) Collection and identification of muskmelon MLO-type genes

[0032] In order to obtain all the members of the MLO gene family in melon, we first constructed an HMM model based on the MLO gene sequences of Arabidopsis thaliana and the MLO gene sequences of tomato, pea, pepper, rose, pepper, and japonicus for resistance to powdery mildew. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC...

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Abstract

The invention relates to rapid identification of MLO (Mycoplasma Like Organism) powdery mildew resistant genes of a melon, relates to comparative genomics of plants, genetics, bioinformatics and other disciplinary knowledge, and belongs to the field of biotechnology science of plants. The rapid identification is mainly carried by the following steps: 1) downloading the whole genome sequence of the melon, and acquiring MLO genes; 2) identifying the MLO genes; 3) obtaining the phylogenetic relationship of MLO genes; 4) comparing MLO powdery mildew resistant genes. By virtue of adopting the rapid identification, the powdery mildew resistant genes mining cycle of the melon can be effectively reduced, which is beneficial to the rapid identification of powdery mildew resistant genes; the identified candidate powdery mildew resistant genes are utilized to develop corresponding coseparation functional markers (such as SNR and SCAR) and can also be quickly used for assistant selection of molecular markers resistant to powdery mildew resistant genes, and the accuracy is high; in combination with other anti-disease gene molecular markers, a plurality of resistive breeding materials can be created, the breeding periods can be reduced, and the breeding efficiency can be increased; the foundation is provided for describing a powdery mildew resistant molecular mechanisms of the melon.

Description

technical field [0001] The present invention is to quickly identify the powdery mildew gene of melon by means of sequencing the whole genome sequence of melon, using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategies, and mainly involves the download of the whole genome sequence of melon and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Melon is also called sweet melon or muskmelon. Melon is named for its sweet taste, and it is also called muskmelon because of its delicate fragrance. Melon is a summer melon to cool off the summer heat, and its nutritional value is comparable to that of watermelon. According to the measurement, except that the content of water and protein in melon is lower than that of watermelon, other nutrients are not less than that of wat...

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68A01H1/02
Inventor 钱春桃其他发明人请求不公开姓名
Owner 南农大(常熟)新农村发展研究院有限公司
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