Rapid identification of powdery mildew gene of Medicago truncatula by utilizing comparative genomics
A powdery mildew resistance gene, powdery mildew resistance technology, applied in genetic engineering, plant genetic improvement, application and other directions, can solve the problems of difficulty in cloning and long time, and achieve the effects of shortening breeding years, shortening time, and improving identification efficiency.
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[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the powdery mildew gene of Medicago truncatula. The specific implementation process is as follows:
[0031] 1) Collection and identification of the MLO gene of Medicago truncatula
[0032] In order to obtain all the MLO-type gene family members of Medicago truncatula, we first constructed an HMM model using the MLO-type genes of Arabidopsis thaliana and the MLO gene sequences of tomato, pea, rose, pepper, and japonicus for powdery mildew resistance. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC3159259, 3 TC TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC2931...
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