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Rapid identification of powdery mildew gene of Medicago truncatula by utilizing comparative genomics

A powdery mildew resistance gene, powdery mildew resistance technology, applied in genetic engineering, plant genetic improvement, application and other directions, can solve the problems of difficulty in cloning and long time, and achieve the effects of shortening breeding years, shortening time, and improving identification efficiency.

Inactive Publication Date: 2012-10-10
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research of Medicago truncatula is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of powdery mildew gene of Medicago truncatula by utilizing comparative genomics
  • Rapid identification of powdery mildew gene of Medicago truncatula by utilizing comparative genomics
  • Rapid identification of powdery mildew gene of Medicago truncatula by utilizing comparative genomics

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Embodiment Construction

[0030] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the powdery mildew gene of Medicago truncatula. The specific implementation process is as follows:

[0031] 1) Collection and identification of the MLO gene of Medicago truncatula

[0032] In order to obtain all the MLO-type gene family members of Medicago truncatula, we first constructed an HMM model using the MLO-type genes of Arabidopsis thaliana and the MLO gene sequences of tomato, pea, rose, pepper, and japonicus for powdery mildew resistance. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC3159259, 3 TC TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC2931...

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Abstract

The present invention is rapid identification of a powdery mildew gene of Medicago truncatula, relates to discipline knowledge of plant comparative genomics, genetics and bioinformatics and belongs to the technical field of plant biotechnology. The invention mainly comprises the following steps: 1) download of Medicago truncatula genome sequence and collection of an MLO gene; 2) identification of MLO type gene; 3) MLO gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew gene. The invention effectively shortens a mining cycle of the powdery mildew gene of Medicago truncatula and facilitates the rapid identification of the powdery mildew gene. The identified candidate powdery mildew genes can be used to develop corresponding coseparation functional markers (SNP and SCAR, etc.), and can also be used quickly for molecular marker assisted selection of powdery mildew resistant gene, and has high accuracy. Combined with other disease resistant gene molecular markers, the identified candidate powdery mildew genes can be used in development of multiresistance breeding material, so as to shorten the breeding period and improve breeding efficiency. The invention lays foundation to elaborate molecular mechanism of powdery mildew resistance of Medicago truncatula.

Description

Technical field [0001] The present invention uses the entire genome sequence of Trumidae, and uses plant comparative genome, genetics, biological information, and candidate genetic strategies to quickly identify the genes of Trumida Plurium Plurium Powder, which mainly involves the download of the whole genome sequence of Trumidaee, candidates for candidatesGene identification, genetic comparison, clustering and other means, which then identified the genetic leather gene, belongs to the field of plant biotechnology science. Background technique [0002] 豆Man powder is mainly used in Doue Leveillula leguminosarum Golov.) And pea white powder ( Lysiphe pisi DC.) Caused that the germs overwintering on the diseased body with closure shells, or wintering with dormant mycelium. After returning to green in the spring of the following year, the initial infection was initially invaded by the subconta spores.In the growth season, the germs were repeatedly invaded by conidia, and the conidi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68A01H5/00
Inventor 钱孝英俞锞
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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