Rapid identification of strawberry powdery mildew gene by using comparative genomics

Abstract

The present invention provides rapid identification of strawberry anti-powdery mildew gene, relates to knowledge of plant comparative genomics, genetics, bioinformatics and other disciplines, and belongs to the plant biotechnology science field. The main steps of the invention comprise: 1) downloading the complete genome sequence of the strawberry, and collecting MLO type gene; 2) identifying the MLO type gene; 3) achieving the MLO type gene phylogeny relationship; and 4) comparing the MLO type powdery mildew gene. According to the invention, the strawberry powdery mildew gene finding period is effectively shortened so as to easily achieve rapid powdery mildew gene identification; the corresponding co-segregation function marker (SNP, SCAR and the like) is developed through the identified candidate powdery mildew gene, the strawberry anti-powdery mildew gene can further be rapidly used for the molecule marker-assisted selection of the anti-powdery mildew gene, and the accuracy is high; the strawberry anti-powdery mildew gene can be combined with other disease resistance gene molecule markers so as to create multi-resistance breeding materials, shorten the breeding period, and improve the breeding efficiency; and the foundation is established for exposition of the strawberry anti-powdery mildew molecule mechanism.

Description

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AI Technical Summary

Benefits of technology

This patented method allows scientists to rapidly identify specific types of bacteria that cause damage or reduce their effectiveness during storage by searching out resistant genes from existing sources like rice plants. By comparing these with known strains found at different locations within the same type of crop grown under similar conditions, researchers are able to make more accurate conclusion about how well they were doing this work compared against older crops without causing any harmful consequences.

Problems solved by technology

The technical problem addressed in this Patented Experimental System relates to identifying specific types of bacterial strains causing pulmonerrilliectatic syndrome or gray leaf spotting symptoms when they grow up inside crop crops without being affected by external factors like weather conditions. Current techniques involve screening out individual sources from each variety and then selecting those having good tolerances against certain pathogens. These approaches require extensive laborious effort and may result in false positives due to poorly understood phenotypic characters.

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