38 results about "Genomic research" patented technology

The invention belongs to the technical field of propagation of pears and particularly discloses an adventitious-bud-grafted rapid propagation method for pears. According to the method, annual or biennial Pyrus betulaefolia bunge or Pyrus calleryana solid-wood seedlings are selected as rootstocks, adventitious buds obtained through tissue culture and subculture propagation of Pyrus sinensis or goldpears are selected as scions, the rootstocks and the scions are pretreated before grafting, then, grafting is carried out in a greenhouse, T-shaped bud grafting is adopted as a grafting method, basalportions of adventitious-bud scion stems are beveled, then, the beveled basal portions of the adventitious-bud stems are dipped with a hormone mixed solution, the dipped adventitious-bud stems are inserted into T-shaped cuts of rootstock stems, grafted ports are subjected to sealing and plastic bag covering treatment, and then, grafted post-management is carried out. According to the adventitious-bud-grafted rapid propagation method for the pears, provided by the invention, culture is carried out in the greenhouse, the operation is simple, the culture period is short, the survival rate is high, the problem that adventitious roots of the pears are difficult in regeneration can be effectively solved, and a technical support is provided for tissue culture regeneration, industrialized seedling rearing and functional genomics research of the pears.

The present invention provides an activation tag Ac / Ds transposition system, comprising a transposase Ac expression vector and a transposon Ds carrier, characterized in that the transposon Ds carrier has an activation tag, and the activation tag is 4 A series of 35S enhancers, 4×35S enhancers are placed inside the 3' end of Ds. The Ac / Ds transposition system of the present invention can express the Ac transposase gene at a high level in various plants, activate the expression level of genes adjacent to the transposon insertion site at a high level, to create dominant mutants, and efficiently identify and Isolation of sequences flanking transposable element insertion sites for mutant library creation in polyploid plants. The invention is an ideal plant functional genomics research tool, and has great significance for the establishment of mutants and functional genomics research of crops, especially polyploid crops.

The invention discloses a Tripterygium wilfordii protoplast separation and instantaneous conversion method. Experiments prove that the yield of protoplasts obtained by the separation method is 4.24*10<5> / g FW, and the activity is 94.5%; and exogenous plasmids are successfully converted into the protoplasts extracted by the invention through a PEG 4000 mediated conversion method and are smoothly expressed, thereby successfully establishing a Tripterygium wilfordii suspension cell protoplast instantaneous conversion system. The invention lays a foundation for carrying out functional genomics research of Tripterygium wilfordii in a deep-going way, and simultaneously provides a material basis for research of Tripterygium wilfordii such as gene editing, subcellular localization, gene overexpression and interference and the like.

The invention belongs to biotechnology field, and relates to a method for developing a dendranthema SSR (Simple Sequence Repeat) primer based on transcriptome sequencing. A lot of sequence information is processed in batch for searching an SSR sequence and designing an SSR labeled primer based on the transcriptome sequencing by utilizing EST-SSR (Expressed Sequence Tags-Simple Sequence Repeat) interspecific metastasis and using a Perl (Practical Extraction and Reporting Language) programming language method in combination, so that the defects of low efficiency, long time consumption, high cost and the like of the SSR development are conquered. Different dendranthema materials are selected for verifying the designed SSR primer, and the primer is a successful SSR primer if detected out by any strip. By adopting the method, 1788 pairs of SSR primers are successively designed, so that a novel method and thinking are provided for the development of the dendranthema SSR primer to further achieve molecular marker assistant selection breeding and comparative genomics research.

The invention discloses a method for inducing haploid endosperm callus tissues by using ripe taxales chinensis var.mairei endosperms, and the method comprises the following steps of: (1) seed exotesta peeling: mechanically breaking a shell, and manually peeling exotesta; (2) explants sterilization: sequentially sterilizing by using 70% alcohol for 90 seconds and 0.1% mercuric chloride for 12 minutes, and washing five times with sterile water for later use; (3) seed pretreatment: sealing with the sterile water, and soaking seeds for 1-5 days at normal temperature; (4) separation of endosperm tissues; and (5) in vitro induction of endosperm callus tissues: inoculating seed endosperms to a callus tissue-inducing culture medium, so as to obtain the 97.92% induction rate after 30 days. By utilizing the method, ripe taxales chinensis var.mairei endosperm callus tissues can be obtained conveniently and quickly, and the ripe taxales chinensis var.mairei endosperm callus tissues lay a foundation for simplifying genetic analysis and chromosome operation and are used as transgenic materials and genomics research materials.