Adventitious-bud-grafted rapid propagation method for pears

Abstract

The invention belongs to the technical field of propagation of pears and particularly discloses an adventitious-bud-grafted rapid propagation method for pears. According to the method, annual or biennial Pyrus betulaefolia bunge or Pyrus calleryana solid-wood seedlings are selected as rootstocks, adventitious buds obtained through tissue culture and subculture propagation of Pyrus sinensis or goldpears are selected as scions, the rootstocks and the scions are pretreated before grafting, then, grafting is carried out in a greenhouse, T-shaped bud grafting is adopted as a grafting method, basalportions of adventitious-bud scion stems are beveled, then, the beveled basal portions of the adventitious-bud stems are dipped with a hormone mixed solution, the dipped adventitious-bud stems are inserted into T-shaped cuts of rootstock stems, grafted ports are subjected to sealing and plastic bag covering treatment, and then, grafted post-management is carried out. According to the adventitious-bud-grafted rapid propagation method for the pears, provided by the invention, culture is carried out in the greenhouse, the operation is simple, the culture period is short, the survival rate is high, the problem that adventitious roots of the pears are difficult in regeneration can be effectively solved, and a technical support is provided for tissue culture regeneration, industrialized seedling rearing and functional genomics research of the pears.

Description

technical field [0001] The invention relates to the technical field of pear seedling cultivation and multiplication, in particular to a method for rapid propagation of pear adventitious bud grafting. Background technique [0002] my country is one of the primary centers of pears, with a long history of cultivation, and now my country's pear production and area are the first in the world, with remarkable economic and social status. Tissue culture is the foundation of the entire life sciences, especially with the development of virus-free rapid propagation technology and functional genomics, the requirements for efficient regeneration systems of tissue culture are also getting higher and higher. Compared with other crops, the development of pear tissue culture technology is relatively late, and its explant regeneration is usually divided into two stages: the first stage is the regeneration of adventitious buds, which can be obtained by primary culture, and can be obtained by m...

AI Technical Summary

Problems solved by technology

Compared with other crops, the development of pear tissue culture technology is relatively late, and its explant regeneration is usually divided into two stages: the first stage is the regeneration of adventitious buds, which can be obtained by primary culture, and can be obtained by multiplication after adventitious buds are obtained. A large amount of clonal material has been obtained, and the regeneration and multiplication problems have basically been solved at this stage; the second stage is the induction and regeneration of adventitious roots, which is relatively difficult, and the regeneration rate is low in the induction of adventitious regenerative roots, and the root development or absorption after regeneration is not enough. Normal, difficult to survive after hardening or slow growth after survival, thus limiting the wide application of tissue culture technology on Pyrus plants

Therefore, how to solve the problems of difficult regeneration of adventitious shoot roots, low regeneration rate and abnormal root development after adventitious bud proliferation has become an urgent problem to be solved in pear tissue culture.

In order to solve the above problems, predecessors have carried out a lot of research, including the direct induction of hormones, test tube grafting, etc., but either the effect is not ideal, or the operation is cumbersome.

For example Chen Lei et al. "Research on Pear Tissue Culture and In-vessel Grafting Technology", which mainly studies the in-vessel (in-test tube) grafting of pears. It needs to go through a long seedling hardening period in the later stage before it can be transplanted outside the bottle. Complicated and slow growth after grafting

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