Tripterygium wilfordii protoplast separation and instantaneous conversion method

A protoplast and tripterygium wilfordii technology, applied in the field of plant tissue culture, can solve the research gaps of triptolide protoplasts and transient transformation, limit the in-depth study of tripterygium wilfordii functional genes and synthetic biology, etc.
CN106399223AActive Publication Date: 2017-02-15CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2017-02-15

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Abstract

The invention discloses a Tripterygium wilfordii protoplast separation and instantaneous conversion method. Experiments prove that the yield of protoplasts obtained by the separation method is 4.24*10<5> / g FW, and the activity is 94.5%; and exogenous plasmids are successfully converted into the protoplasts extracted by the invention through a PEG 4000 mediated conversion method and are smoothly expressed, thereby successfully establishing a Tripterygium wilfordii suspension cell protoplast instantaneous conversion system. The invention lays a foundation for carrying out functional genomics research of Tripterygium wilfordii in a deep-going way, and simultaneously provides a material basis for research of Tripterygium wilfordii such as gene editing, subcellular localization, gene overexpression and interference and the like.
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Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and specifically relates to a method for separating and transiently transforming protoplasts of Tripterygium wilfordii, in particular to a method for separating and purifying protoplasts of Tripterygium wilfordii and a method for transiently transforming exogenous genes. Background technique

[0002] The traditional Chinese medicine Tripterygium wilfordii Hook.f. is a root medicinal material, which is derived from the root or xylem of the root of Tripterygium wilfordii Hook.f.. and other effects. The main active substances of Tripterygium wilfordii are alkaloids, diterpenes, triterpenes, sesquiterpenes and sugars. It has anti-inflammatory, anti-tumor, immunosuppressive and other effects, and is mainly used in rheumatoid, anti-tumor, glomerular diseases, systemic lupus erythematosus and other diseases in modern clinical treatment. In recent years, the study of Tripterygium wilfordii has attra...

Claims

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