Anther development control gene and use thereof in realizing male sterility in Arabidopsis thaliana

Abstract

The invention relates to an anther development control gene and use thereof in realizing male sterility in Arabidopsis thaliana. An Arabidopsis thaliana male sterile mutant ahl16 is screened out from T-DNA-inserted mutant population, a gene AHL16 controlling the fertility of Arabidopsis thaliana is cloned and identified, the nucleotide sequence of the gene AHL16 is represented by SEQ ID N0.1, and genetic complement experiments prove that the AHL16 gene in a wild type can restore the male sterile phenotype. Amino acid sequence analysis and subcellular localization experiments indicate that the AHL16 gene codes proteins of an AT-hookmotif nuclear localized protein family and is located in the nucleus. In Arabidopsis thaliana, the mutation of the gene leads to complete male sterility. The gene has a very important application value in aspects of explanation of influences of the growth of the inner layers of the outer walls of microspores and the structures of the inner walls on the fertility of plants and improvement on yield by hybrid seed production.

Description

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to a gene for regulating the development of plant anthers to regulate fertility, encoded protein and application thereof. Background technique

[0002] The sexual reproduction of plants is not only the main way of plant reproduction, but also one of the foundations of plant evolution and adaptation to the environment. Anthers are the male reproductive organs of plants, where pollen develops. The development of anther and pollen is an important research direction in the study of plant gene function. Abnormal development of anthers and pollen will cause anthers to fail to produce normal functioning male gametes, resulting in male sterility. Male sterility is the basis of hybrid seed production in production. Therefore, the in-depth study on anther and pollen development has both theoretical research significance and practical application value.

[0003] The tapetum is...

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