A method for constructing Torreya est-ssr primer and variety fingerprint

A technology of Torreya and primer sequences, which is applied in the field of Torreya EST-SSR primers and the construction of variety fingerprints, can solve the problems of difficulty in statistical analysis, large amount of DNA, long cycle, etc., and achieves low DNA quality requirements and high polymorphism. , high repeatability
CN108359738BActive Publication Date: 2021-08-27ZHEJIANG FORESTRY UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG FORESTRY UNIVERSITY
Publication Date
2021-08-27

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Abstract

The invention belongs to the technical field of molecular fingerprints, and discloses a Torreya EST-SSR primer and a method for constructing a variety fingerprint. The sequence is: SEQ ID NO: 1-SEQ ID NO: 40. The screened 20 polymorphic EST-SSR markers were used to amplify the DNA of 13 torreya species by PCR; according to the amplification results, the length of the amplified fragment was accurately read, and after statistics, at least three pairs of EST-SSR were used The combination of primers distinguished the 13 Torreya species tested. Based on the transcriptome data of 'Torreya' and 'Torreya' seeds, the present invention analyzes the obtained SSR data, screens out SSR primers with clear amplified bands and high polymorphism, and uses them to construct characteristic varieties of Torreya At the same time, it provides a good basis for the evaluation, preservation and utilization of Torreya germplasm resources.
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Description

technical field

[0001] The invention belongs to the technical field of molecular fingerprints, in particular to a Torreya EST-SSR primer and a method for constructing a variety fingerprint. Background technique

[0002] Torreya (Torreya) was founded by Arnott in 1938, and Florida Torreya (Torreya taxifolia Arn) is the model plant of this genus. According to literature records, there are only 7 species and 2 varieties of Torreya extant, distributed intermittently in East Asia and North America. Among them, Torreya taxifolia (Torreya taxifolia) and Torreya californica Torrey (Torreya californica Torrey) are produced in North America, and Japanese Torreya (Torreya nucifera (L.) Sieb.et Zucc.) is wildly distributed in Japan and the Korean Peninsula. Some cities in my country are introduced as garden trees. cultivation. Torreya fargesii Franch., Torreya jackii Chun, Torreya grandis Fort.ex Lind, Torreya parvifolia Yi, L Yang et Long, Torreya fargesii Franch.Var.yunnanensis) and...

Claims

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