The invention discloses a method of utilizing a
chemistry-
enzyme method to produce L-
glufosinate ammonium. According to the method, racemic N-acetyl
glufosinate ammonium is taken as the substrate,
engineering bacteria containing carboxyl-peptidase genes are subjected to
fermentation culture to obtain wet cells, wet cells or pure enzymes, which are obtained by steps of
grinding wet cells through ultrasonic
waves and extracting grinded wet cells, are taken as the catalyst, a
buffer solution with a pH value of 5-10 is taken as the reaction medium, reactions are performed in a water bath with a temperature of 45 DEG C at a rotation speed of 200 rpm, after complete reactions, a reaction solution containing D-N-acetyl
glufosinate ammonium and L-
glufosinate ammonium is obtained, the reaction solution is separated and purified, and collected D-N-acetyl
glufosinate ammonium is subjected to
racemization and then split in cycles to obtain L-
glufosinate ammonium at the same time. The provided method does not need a coenzyme circulation
system or an amino donor with a structure that is similar with the product; the reaction steps are simple, the
reaction product is easy to separate, the opticalselectivity is high (ee value is 99%), the separation effect of the
ion exchange column is obvious, and L-glufosinate ammonium with higher purity can be obtained more easily (purity is 98%).