49 results about "Plant Anthers" patented technology

The invention discloses a plant anther-specific promoter and application thereof. The promoter is 179 to 1174 bp in length at least containing the 996-1174th nucleotide sequence at the 5' end of sequence 1 in the sequence listing at the 3' end and extending to the 5' end according to the nucleotide arrangement of sequence 1 deoxynucleotide fragments. The anther-specific promoter of the present invention has great application prospects in the functional analysis and identification of plant anther growth and development genes, the establishment of artificial male sterile lines, the prevention of plant transgene drift or escape, and the extension of shelf life of flowers.

The invention relates to genetic engineering and molecular biology, and particularly discloses a plant anther specific promoter PCHF15 and application thereof. The invention further provides an expression vector with the plant anther specific promoter PCHF15, a genetic expression box and a carrier with the genetic expression box. The invention also provides a primer pair for amplifying the plant anther specific promoter PCHF15. The plant anther specific promoter PCHF15 is an endogenous gene of rice, is beneficial to genetic engineering of the rice, and drives exogenous genes to accurately express specificity in pollen, so that a novel method for driving specificity expression of the exogenous genes in the pollen is provided.

The invention discloses plant anther specificity promoter and its application. The promoter nucleotide sequence can be the one as (1) sequence table number one in question; or the one as (2), (1) in question nucleotide sequence complementary; or the one as (3), (1), or (2) in question which has 60% or above homology; or the one as (4), (1), (2), or (3) in question which can hybridized at strict hybridization condition. The inverse gene, small RNA, or expression vector can be used to transform host cell or tissue and its progeny cell to culture male sterile line plant, prevent transgene from polluting its wild species, landscaping plant, or crop species, etc.

The invention provides a specific expression promoter OsAnth1 for anthers and an application thereof. The promoter which can carry out a specific expression in the plant anthers is departed, and the promoter can guide a target gene to carry out a specific high expression in the plant anthers and carry out a low expression or not express in other issues of a plant. The invention further provides an expression box containing the promoter, a plant expression carrier and a method for obtaining corresponding host bacteria and converters by using the promoter. In addition, the promoter is applied to plant gene engineering. The promoter has a significant theory and an actual meaning for relevant researches about the rice anthers. The wide application and the wide market prospect are going to be obtained in the field of agriculture.

The invention discloses a method for preparing a cabbage type rape BnPABP3 promoter (PBnPABP3) and the application thereof. The method comprises the following steps of: cloning PBnPABP3 sequence by utilizing the genome walking method, extracting genome DNA by utilizing the SDS cracking method, carrying out enzyme digestion on DNA by respectively using endonuclease DraI, EcoRV, PvuII and StuI in different systems, carrying out the first round of PCR amplification by adopting the product as a template after joint segments are connected with the product, and carrying out the second round of PCR amplification by adopting the 50 times diluent of PCR products obtained in the first round as a template so as to obtain the PBnPABP3. The PBnPABP3 is applied to anther and pollen grains of plant. The promoter has the function of driving exogenous gene expression, the expression parts of the promoter are in the anther and pollen grains of plant, and the promoter has the application potentiality in the aspects of improving the safety of rape edible oil as well as the quality of crops, artificially creating sterile line and restorer line, enriching germ plasm resources, and the like.

This invention describes novel DNA sequences which function as promoters of anther-specific transcription of coding DNA sequences in recombinant or chimeric DNA sequences. The invention also describes recombinant or chimeric DNA sequences, which are expressed specifically in the anther of a plant. The said recombinant or chimeric DNA sequences may be used to create transgenic plants, but especially transgenic male-sterile plants.

The invention relates to a tissue-specific promoter and an application thereof. A nucleotide sequence of the tissue-specific promoter comprises a sequence represented by (a) or (b) as follows: (a) a sequence comprising nucleotide sequences represented by nucleotide at positions 920-932, nucleotide at positions 947-961, nucleotide at positions 1507-1519, nucleotide at positions 1963-1971 and nucleotide at positions 2018-2028 in SEQ ID NO:1 simultaneously; (b) a nucleotide sequence hybridized with the nucleotide sequences limited by (a) under stringent conditions. The tissue-specific promoter is isolated from an ABCG15 gene of a nonglutinous rice variety 93-11 for the first time; in addition, order-adjustable heterogeneous nucleotide sequences are specifically expressed in plant anther tissue, particularly tapetum tissue, so that a foundation is laid for creation of a novel male sterility line.

The invention relates to a dyeing method for observing anther development structure changes of plants. By use of the dyeing method, the problem of clearly observing of an anther wall tapetum and structure changes and an anther wall can be effectively solved. The dyeing method comprises the following steps: fixing an anther by using a mixed solution containing formaldehyde, glacial acetic acid and alcohol, performing dehydration by using alcohol, then treating the anther for three times in sequence in a mixed solution containing n-butyl alcohol and alcohol, and soaking the anther in n-butyl alcohol; then transferring the anther into a mixed solution containing n-butyl alcohol and paraffin, pouring out half of the mixed solution and adding an equal weight of paraffin every 3h, and finally, pouring out the mixed solution, embedding the anther into a paraffin block by using paraffin, and cutting the paraffin block into a paraffin belt; and coating gelatin on a glass slide, attaching the paraffin belt to the glass slide, baking the paraffin belt, performing dewaxing, then treating the glass slide once in alcohol and distilled water respectively, treating the glass slide with the anther in periodic acid, rinsing the glass slide by using distilled water, performing dyeing in a Schiff reagent, rinsing the glass slide by using distilled water, and covering the glass slide with another glass slide. The dyeing method provided by the invention is easy to operate, good in dyeing effect, and clear and accurate in observation of the anther development structure changes of the plants.

The invention discloses an early anther specific promotor and an application thereof. The promoter is the promoter of a rice gene LOC_Os05g42240; the promoter can be used for controlling the specific expression of a target gene in such cells as anther primordium, tapetum and microspore cells in an anther early development stage of a plant; and the promoter is applicable to researches in the aspects of plant breeding and gene function analysis.

The invention discloses plant anther pollen development late-stage specific expression promoters pOsLSP and application thereof, belongs to the technical field of plant biology, and particularly relates to application of rice anther pollen development late-stage specific expression promoters in regulation of male sterility of rice. Nucleotide sequences of pOsLSP5 and pOsLSP6 promoters of rice Nipponbare are cloned and embedded with an amyloplast transport signal peptide gene BT1 (TP) and an alpha-amylase gene ZM-AA1 respectively to construct a recombinant expression vector and perform genetictransformation. Under the drive of the promoters pOsLSP5 and the guide of the amyloplast transport signal peptide, the alpha-amylase gene can be expressed in late-stage pollen and hydrolyze starch tocause transgenic pollen sterility, and the promoters pOsLSP have high accuracy, can be applied to maintenance of homozygous recessive state of male sterile plants and mass expanding propagation of sterile lines, omit an artificial castration step in a hybrid seed production process, and have broad application prospects in crop planting resource improvement, genetic breeding and other aspects.

The invention discloses a rape BnPAB5 gene and application of the rape BnPAB5 gene. A separated gene has the sequence as shown in SEQ ID No.1 and SE1 ID No.2. The PAB5 in combination with the poly(A) tail plays a special role in the transcript processes, such as starting of polyadenylation, transporting and translation, thus the expression processes of other genes can be controlled. The BnPAB5 shows preferential expression in rape anther and microspore. The expression of Arabidopsis AtPAB5 is interfered to result in abortion of the microspore. The transgenic arabidopsis of overexpression BnPAB5 is utilized as a male parent and pollinated to the transgenic arabidopsis of the interference AtPAB5, and the fertility of plant anther of filial generation and pollen can be recovered. As the complementary test shown, the rape BnPAB5 and the arabidopsis AtPAB5 have the same functions, such as the functions of controlling the development of the rape anther and arabidopsis anther and the fertility of the pollen; the expression level of the PAB5 can be controlled by genetic engineering technology, thus the fertility of the pollen can be controlled, and male sterility line materials and restoring line materials can be created; and the rape BnPAB5 gene can be applied to the study on male gametes development and the improvement of quality of crop.

The invention provides a method for preserving the callus of rubber plant anther with vitrification and super low temperature, comprising the following steps: the anther callus is inoculated to the pre-culture medium, dark culture is carried out for 2 to 3 days with the culture temperature of 25 to 28 DEG C; the anther callus is processed by 60 percent of PVS2 for 10 to 30 minutes at room temperature; after dehydration process, the anther callus is processed by the PVS2 for 20 to 40 minutes at the temperature of 0 DEG C, the stock solution of the PVS2 is removed, then new PVS2 is added, and the anther callus is stocked in liquid nitrogen; and after the anther callus is resurgent, successive transfer culture is carried out, the regeneration plant can be obtained after further embryoid is induced and the mature embryoid is induced to carry out differentiation. The preservation method by liquid nitrogen with vitrification and super low temperature related by the invention has the advantages of simple equipment, simple experiment procedure and good effect and repetitiveness, etc., can preserve the callus of rubber plant anther for long time, obtains the regeneration plant by culturing after unfrozen and creates conditions for the genetic transformation of the rubber plant.

The invention discloses a cotton casein kinase gene and its application, and relates to a separation cloning, a function verification and an application of a gene GhCKI used for controlling anther growth and identified in a cotton anther expression spectrum. There is a 72% homology between the protein coded by the GhCKI gene and the protein coded by an Arabidopis thaliana ATCKL2 gene, and the GhCKI gene preferentially expresses in the anther, has an anther growth controlling function, can be applied to the sterile line cultivation of a plurality of plants through genetic transformation, and simultaneously can be used as a marker gene of the plant anther fertility stability under high temperature stress.

The invention discloses a method of male sterility in plant by artificial hybridization, which is characterized in that: the method is a method which inhibits the expression of an endogenous HSP70 gene of plant anther through the antisense RNA technology, and interferes the normal growth of the transgenic plant anther so as to acquire a male sterility transgenic plant. In particular, the method is a method which transforms an antisense RNA expression vector of the HSP70 gene driven by an Osg6B promoter expressed by anther specificity into a graminaceous plant, inhibits the expression of the endogenous HSP70 gene of the anther, and interferes the normal growth of the transgenic plant anther so as to acquire the male sterility transgenic plant. The transgenic plant acquired by the method can implement male complete sterility.

The invention belongs to the technical field of plant genetic engineering and provides a promoter sequence for the specificity expression of plant anthers, a recombined nucleic acid sequence containing a promoter, a construction body and an expression system, wherein the promoter can drive functional genes to express the specificity in the plant anthers.

The invention provides a determination method for the mass of a plant anther and pollen. The determination method comprises the following steps: collecting flower buds; collecting an anther with unloosened pollen and weighing the fresh weight of the pollen; putting the pollen into a weighing bottle which is dried and weighed and can be sealed; weighing the total weight of the pollen and the weighing bottle; drying the sealed weighing bottle filled with the pollen to a constant weight; weighing the total dry weight of the pollen and the weighing bottle; then transferring the dried pollen to a leaking sieve and washing to remove pollen particles; then drying pollen shells and weighing the dry weight of the pollen shells; and finally, calculating the dry weights of a single anther and the pollen thereof. According to the determination method, the dry weights of the anther and the pollen are quantitatively estimated by measuring the water content of the anther and the mass of the pollen shell, so as to provide a novel method for scientifically and reasonably estimating male reproduction distribution of plants.

The invention discloses a specific promoter GmFTL2 for anthers, ovules and root caps of soybeans. The nucleotide sequence of the specific promoter GmFTL2 is shown as SEQ NO:1. The invention further provides an application of the promoter in regulation of downstream gene expression. The promoter GmFTL2 is used for overexpressing a GUS gene in arabidopsis thaliana, and results show that the promoter GmFTL2 can obviously increase expression quantity of the GUS gene in anthers, ovules and root caps of plants, so that the promoter GmFTL2 has specificity in the anthers, the ovules and the root caps of the plants and is suitable for spherically expressing a target gene in the anthers, the ovules and the root caps of the plants such as various crops, forests, vegetables, flowers, grass and the like.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention provides an anther-specific expression promoter in plant, wherein said promoter is a promoter of Oncidium aureusidin synthase gene OgAS1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that comprised a promoter having a DNA sequence as SEQ ID No: 3, and a polynucleotide that encode an open reading frame and is linked to the 3′ end of said promoter, wherein said promoter can activate the transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides also a gene expression vector that contains a promoter having DNA sequence as SEQ ID No: 3. The invention provides further a process for producing a transgenic plant or part of organ, tissue or cell of said transgenic plant containing the above-described gene expression cassette.

The invention belongs to the technical field of plant biology, discloses identification and application of a plant anther specific expression promoter pTaASG005 and particularly relates to separation, function identification and application of the plant anther specific expression promoter. The promoter is specifically expressed in plant anthers, particularly in anthers of pollen in a uninucleate stage, a binucleate stage and a trinucleate stage. The plant anther specific expression promoter pTaASG005 has a promising application prospect in the field of plant transgene.

The invention relates to the field of plant molecular biology, in particular to an expression cassette and a vector for recovering male fertility of rice OsCYP704B2 mutant as well as a detection method and application of the expression cassette and the vector. The invention provides a plant anther promoter as shown in SEQ ID NO.1 and the rice OsCYP704B2 gene expression cassette which is driven bythe promoter and is subjected to sequence optimization. The anther promoter provided by the invention can drive genes to be efficiently expressed in plant anther; according to the rice OsCYP704B2 geneexpression cassette provided by the invention, high-level expression of the OsCYP704B2 gene in anther under the driving of the anther promoter can be realized; the rice male sterility caused by OsCYP704B2 function deletion is completely recovered, and the rice male sterility promoter has high genetic stability and can be used for recovering rice male fertility or preparing a rice male genic malesterility maintainer line and a sterile line.

The invention relates to the field of genetic engineering, in particular to a relevant protein TaAOC for regulating and controlling the cracking of a plant anther as well as a gene and application thereof. The invention provides the relevant protein TaAOC for regulating and controlling the cracking of the plant anther. The amino acid sequence of the relevant protein TaAOC is shown as SEQ ID NO.1.In addition, the invention also provides the gene for coding the relevant protein for regulating and controlling the cracking of the plant anther. The amino acid sequence of the gene is shown as SEQ ID NO.2 or SEQ ID NO.3. Furthermore, the invention provides a recombinant vector containing the gene and application of the relevant protein TaAOC. The gene provided by the invention is correlated with the fertility of the plants, can be used for improving the cracking rate and the setting percentage of the plant anther and has very important theoretical and actual significances for increasing theyield of grains and developing the two-line hybrid wheat theory.

The invention relates to a carrier of the male fertility of a recoverable rice OsCYP704B2 mutant containing an Oryza Punctata promoter and an application thereof. The nucleotide sequence of the OryzaPunctata gene OpCYP704B2 promoter is shown by SEQ ID NO.1. The nucleotide sequence of a rice gene OsCYP704B2 expression cassette driven by the promoter and optimized in sequence is shown by SEQ ID NO.4. The promoter provided by the invention can drive genes to express efficiently in plant anthers. The rice gene OsCYP704B2 expression cassette provided by the invention can achieve gene OsCYP704B2 being driven by the promoter to express in the anthers in a high level and fully recovering rice male sterility caused by the lack of OsCYP704B2 functions, has high hereditary stability and can be usedfor recovering the male fertility of rice or preparing nucleic male sterility maintainer lines and male sterile lines of the rice.

The invention belongs to the technical field of plant biology and particularly relates to isolation, functional identification and application of a promoter pTaASG019 featuring plant anther-specific expression. The promoter featuring plant anther-specific expression has promising application prospect in the field of plant transgenosis.

The invention belongs to the field of plant biotechnology, and relates to the separation and application of plant tissue specific promoters, in particular to application of a rape tissue specific promoter for regulating target genes expressed in plant anther. The promoter and the truncated fragments of the promoter are integrated with the reported genes and transformed into arabidopsis thaliana, and can drive the reported genes to express specifically in anther by GUS staining, and the sequences are shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4; after further truncation, a promoter fragment with a sequence of pollen specific expression shown as SEQ ID NO.5 is obtained. The invention further discloses that the tissue specific promoter or the truncated promoter fragments provide new effective regulatory elements for controlling plant fertility, and have a good application prospect for genetic engineering breeding.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.

The invention discloses a plant anther-specific promoter and application thereof. The nucleotide sequence of the promoter is: (1), the nucleotide sequence described in Sequence 1 in the sequence listing; or (2), the nucleotide sequence complementary to the nucleotide sequence described in (1); Or (3), a nucleotide sequence having 60% or more homology with the nucleotide sequence described in (1) or (2); or (4), with (1), (2) or (3) The nucleotide sequence is a nucleotide sequence capable of hybridizing under stringent hybridization conditions. The expression vector containing the promoter and the antisense gene of the endogenous gene, the small RNA capable of interfering with the expression of the endogenous gene, or the fusion fragment of the exogenous gene can be used to transform the host cell or host tissue and its progeny cells to cultivate male sterile plants , Transgenic plants that can prevent transgenes from polluting wild species and related species through pollen drift, and landscaping plants or crop varieties that eliminate or greatly reduce "flying catkins".

The invention relates to the field of genetic engineering, in particular to a relevant protein TaOPR for regulating and controlling the cracking of a plant anther as well as a gene and application thereof. The invention provides the relevant protein TaOPR for regulating and controlling the cracking of the plant anther. The amino acid sequence of the relevant protein TaOPR is shown as SEQ ID NO.1.In addition, the invention also provides the gene for coding the relevant protein for regulating and controlling the cracking of the plant anther. The amino acid sequence of the gene is shown as SEQ ID NO.2 or SEQ ID NO.3. Furthermore, the invention provides a recombinant vector containing the gene and application of the relevant protein TaOPR. The gene provided by the invention is correlated with the fertility of the plants, can be used for improving the cracking rate and the setting percentage of the plant anther and has very important theoretical and actual significances for increasing theyield of grains and developing the two-line hybrid wheat theory.