The invention discloses a plant anther-specific promoter and application thereof. The nucleotide sequence of the promoter is: (1), the nucleotide sequence described in Sequence 1 in the sequence listing; or (2), the nucleotide sequence complementary to the nucleotide sequence described in (1); Or (3), a nucleotide sequence having 60% or more homology with the nucleotide sequence described in (1) or (2); or (4), with (1), (2) or (3) The nucleotide sequence is a nucleotide sequence capable of hybridizing under stringent hybridization conditions. The expression vector containing the promoter and the antisense gene of the endogenous gene, the small RNA capable of interfering with the expression of the endogenous gene, or the fusion fragment of the exogenous gene can be used to transform the host cell or host tissue and its progeny cells to cultivate male sterile plants , Transgenic plants that can prevent transgenes from polluting wild species and related species through pollen drift, and landscaping plants or crop varieties that eliminate or greatly reduce "flying catkins".