Specific expression promoter OsAnth1 for rice anthers

An anther-specific, promoter technology is applied in the fields of biotechnology and plant genetic engineering, which can solve the problems of limiting the ability to improve anther traits and the lack of abundant genetic resources, and achieve the goal of avoiding waste of material and energy, improving growth characteristics and enhancing resistance. Effect

Active Publication Date: 2015-09-30
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0006] However, the genetic resources of anther-specific promoters discovered by

Method used

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  • Specific expression promoter OsAnth1 for rice anthers
  • Specific expression promoter OsAnth1 for rice anthers
  • Specific expression promoter OsAnth1 for rice anthers

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Embodiment Construction

[0031] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0032] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0033] Acquisition of OsAnth1 Promoter Containing Restriction Sites

[0034]Step 1. Design of primers

[0035] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsAnth1 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[00...

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Abstract

The invention provides a specific expression promoter OsAnth1 for anthers and an application thereof. The promoter which can carry out a specific expression in the plant anthers is departed, and the promoter can guide a target gene to carry out a specific high expression in the plant anthers and carry out a low expression or not express in other issues of a plant. The invention further provides an expression box containing the promoter, a plant expression carrier and a method for obtaining corresponding host bacteria and converters by using the promoter. In addition, the promoter is applied to plant gene engineering. The promoter has a significant theory and an actual meaning for relevant researches about the rice anthers. The wide application and the wide market prospect are going to be obtained in the field of agriculture.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice anther-specific expression promoter OsAnth1 and its application. The promoter can drive the specific expression of a target gene in rice anthers in a rice transgene regulation system. Background technique [0002] The exogenous DNA sequence is linked to a specific promoter to promote expression in the plant host, and the choice of the promoter type determines the time and location of gene expression. Plant gene regulation is mainly carried out at the transcriptional level, which is coordinated by a variety of cis-acting elements and trans-acting factors. The promoter is an important cis-acting element. It is a DNA sequence located in the upstream region of the 5' end of the structural gene to regulate gene transcription. It can activate RNA polymerase to accurately combine with the template DNA to ensure a...

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12N15/82C12N5/10A01H5/00
Inventor 秦瑞英杨剑波李浩李莉李娟魏鹏程许蓉芳马卉杨亚春
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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