Plant anther pollen development late-stage specific expression promoters and application thereof

A technology for pollen development and specificity, applied in the field of DNA application and isolated DNA, can solve the problems of long waiting time, high homology gene silencing, and plant growth and development effects.
CN111154756APending Publication Date: 2020-05-15SHENZHEN INST OF MOLECULAR CROP DESIGN +1
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SHENZHEN INST OF MOLECULAR CROP DESIGN
Publication Date
2020-05-15

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Abstract

The invention discloses plant anther pollen development late-stage specific expression promoters pOsLSP and application thereof, belongs to the technical field of plant biology, and particularly relates to application of rice anther pollen development late-stage specific expression promoters in regulation of male sterility of rice. Nucleotide sequences of pOsLSP5 and pOsLSP6 promoters of rice Nipponbare are cloned and embedded with an amyloplast transport signal peptide gene BT1 (TP) and an alpha-amylase gene ZM-AA1 respectively to construct a recombinant expression vector and perform genetictransformation. Under the drive of the promoters pOsLSP5 and the guide of the amyloplast transport signal peptide, the alpha-amylase gene can be expressed in late-stage pollen and hydrolyze starch tocause transgenic pollen sterility, and the promoters pOsLSP have high accuracy, can be applied to maintenance of homozygous recessive state of male sterile plants and mass expanding propagation of sterile lines, omit an artificial castration step in a hybrid seed production process, and have broad application prospects in crop planting resource improvement, genetic breeding and other aspects.
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Description

technical field

[0001] The present invention belongs to the field of plant biotechnology, in particular, the present invention relates to isolated DNA, which can direct the specific transcription and / or expression of nucleic acid operably linked downstream of it in plant anther pollen. In addition, the present invention also relates to expression cassettes and plants containing the DNA, and to applications of the DNA. Background technique

[0002] Plant gene regulation is mainly carried out at the transcriptional level, which is coordinated by a variety of cis-acting elements and trans-acting factors. The promoter is an important cis-acting element. It is a DNA sequence located in the upstream region of the 5' end of the structural gene to regulate gene transcription. It can activate RNA polymerase to accurately combine with the template DNA to ensure accurate and efficient transcription. play a key role in transcriptional regulation. According to the different characteris...

Claims

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