Plant anther specific promoter PCHF15 and application thereof

An anther-specific and promoter technology, applied in the fields of genetic engineering and molecular biology, to achieve precise expression levels
CN106434673AActive Publication Date: 2017-02-22HAINAN BOLIAN RICE GENE TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN BOLIAN RICE GENE TECH CO LTD
Publication Date
2017-02-22

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Abstract

The invention relates to genetic engineering and molecular biology, and particularly discloses a plant anther specific promoter PCHF15 and application thereof. The invention further provides an expression vector with the plant anther specific promoter PCHF15, a genetic expression box and a carrier with the genetic expression box. The invention also provides a primer pair for amplifying the plant anther specific promoter PCHF15. The plant anther specific promoter PCHF15 is an endogenous gene of rice, is beneficial to genetic engineering of the rice, and drives exogenous genes to accurately express specificity in pollen, so that a novel method for driving specificity expression of the exogenous genes in the pollen is provided.
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Description

technical field

[0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a plant anther-specific promoter PCHF15 and its application. Background technique

[0002] Plant genes are mainly regulated at the transcriptional level, which is coordinated between various cis-acting elements and trans-acting factors. The promoter is an important regulatory element in plant gene transcription, generally located in the upstream region of the 5' end of the structural gene, and is the recognition and binding site of RNA polymerase and some trans-acting factors. Promoter is the regulatory center of gene transcription. In-depth study of promoter is not only conducive to mastering the control of gene transcription mode and regulatory mechanism, but also conducive to the regulation of exogenous gene expression. The promoter mainly has two regions, which are the core promoter region and the transcriptional regulatory region. The core promoter...

Claims

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