Determination method for mass of plant anther and pollen

A measurement method, a technology of plant flowers, applied in the direction of weighing by removing certain components, can solve the problems of easy scattering of pollen, no estimation of male reproductive allocation, etc., and achieve easy promotion, good application value, simple and effective method Effect

Inactive Publication Date: 2014-02-26
GANSU AGRI UNIV
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Problems solved by technology

However, in the field of plant ecology, the trade-off between quality and quantity is universal. However, due to the natural dehiscence of plant...

Method used

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  • Determination method for mass of plant anther and pollen

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Embodiment Construction

[0014] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0015] Due to the natural dehiscence of plant anthers after maturity, the pollen is easily scattered, so it is difficult to directly estimate their mass (especially the dry weight). The method of the invention indirectly estimates the quality of the anther and pollen mainly through the measurement of the water content of the anther and the quality of the remaining anther shell after loosening the pollen.

[0016] The flow chart of the inventive method sees figure 1 .

[0017] The concrete steps of the inventive method are as follows:

[0018] During the full flowering period of the plant, a number of flower buds (the anthers have not yet cracked) that are ready to be released are collected. According to the size of the anthers, one sample fo...

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Abstract

The invention provides a determination method for the mass of a plant anther and pollen. The determination method comprises the following steps: collecting flower buds; collecting an anther with unloosened pollen and weighing the fresh weight of the pollen; putting the pollen into a weighing bottle which is dried and weighed and can be sealed; weighing the total weight of the pollen and the weighing bottle; drying the sealed weighing bottle filled with the pollen to a constant weight; weighing the total dry weight of the pollen and the weighing bottle; then transferring the dried pollen to a leaking sieve and washing to remove pollen particles; then drying pollen shells and weighing the dry weight of the pollen shells; and finally, calculating the dry weights of a single anther and the pollen thereof. According to the determination method, the dry weights of the anther and the pollen are quantitatively estimated by measuring the water content of the anther and the mass of the pollen shell, so as to provide a novel method for scientifically and reasonably estimating male reproduction distribution of plants.

Description

technical field [0001] The invention relates to a method for measuring the quality of plant anthers and pollen. Background technique [0002] The pollen production of a plant represents the size of the male reproductive allocation of the species. At present, the method of pollen counting is commonly used to measure the pollen production of plants and estimate their male reproductive input. However, in the field of plant ecology, the trade-off between quality and quantity is universal. However, due to the natural cracking of plant anthers after they mature, pollen is easily scattered. So far, there is no method for estimating male reproductive allocation based on pollen quality. Contents of the invention [0003] The invention provides a method for measuring the quality of plant anthers and pollen. The method is comprehensively applied with the pollen counting technology, and can scientifically and rationally estimate the size of male reproductive allocation of plants. ...

Claims

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Application Information

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IPC IPC(8): G01N5/04
Inventor 张勃侯向阳赵晓倩方强恩陈海军侯军军南丽丽
Owner GANSU AGRI UNIV
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