Determination method of plant anther and pollen quality

A determination method and plant flower technology, which are applied in the direction of removing certain components and weighing, etc., can solve the problems of unestimated male reproductive distribution and easy dispersion of pollen, and have good application value, easy popularization, and simple and effective method. Effect

Inactive Publication Date: 2015-09-23
GANSU AGRI UNIV
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Problems solved by technology

However, in the field of plant ecology, the trade-off between quality and quantity is universal. However, due to the natural dehiscence of plant anthers after they mature, the pollen is easily scattered. So far, there is no method to estimate the male reproductive allocation by pollen quality.

Method used

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  • Determination method of plant anther and pollen quality

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Embodiment Construction

[0014] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0015] Due to the natural dehiscence of plant anthers after maturity, the pollen is easily scattered, so it is difficult to directly estimate their mass (especially the dry weight). The method of the invention indirectly estimates the quality of the anther and pollen mainly through the measurement of the water content of the anther and the quality of the remaining anther shell after loosening the pollen.

[0016] The flow chart of the inventive method sees figure 1 .

[0017] The concrete steps of the inventive method are as follows:

[0018] During the full flowering period of the plant, a number of flower buds (the anthers have not yet cracked) that are ready to be released are collected. According to the size of the anthers, one sample fo...

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Abstract

The invention provides a method for measuring the quality of plant anthers and pollen. The steps are as follows: collect buds that are ready to be released, collect unscattered anthers, weigh their fresh weight, and place them in a dry and weighable sealable weighing scale. In the measuring bottle, weigh the total weight of the anthers and the weighing bottle; dry the sealed weighing bottle containing the anthers to a constant weight, and measure the total dry weight of the anthers and the weighing bottle; then, the dried anthers Transfer to a leaking sieve for washing, remove the pollen grains, then dry the anther sac shell, and weigh its dry weight; finally, calculate the dry weight of a single anther and its pollen. The invention quantitatively estimates the dry weight of anthers and pollen by measuring the water content of the anthers and the quality of the anther shell, and provides a new method for scientifically and rationally estimating the male reproductive allocation of plants.

Description

technical field [0001] The invention relates to a method for measuring the quality of plant anthers and pollen. Background technique [0002] The pollen production of a plant represents the size of the male reproductive allocation of the species. At present, the method of pollen counting is commonly used to measure the pollen production of plants and estimate their male reproductive input. However, in the field of plant ecology, the trade-off between quality and quantity is universal. However, due to the natural cracking of plant anthers after they mature, pollen is easily scattered. So far, there is no method for estimating male reproductive allocation based on pollen quality. Contents of the invention [0003] The invention provides a method for measuring the quality of plant anthers and pollen. The method is comprehensively applied with the pollen counting technology, and can scientifically and rationally estimate the size of male reproductive allocation of plants. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/04
Inventor 张勃侯向阳赵晓倩方强恩陈海军侯军军南丽丽
Owner GANSU AGRI UNIV
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