Carrier of male fertility of recoverable rice OsCYP704B2 mutant containing Oryza Punctata promoter and application thereof

Abstract

The invention relates to a carrier of the male fertility of a recoverable rice OsCYP704B2 mutant containing an Oryza Punctata promoter and an application thereof. The nucleotide sequence of the OryzaPunctata gene OpCYP704B2 promoter is shown by SEQ ID NO.1. The nucleotide sequence of a rice gene OsCYP704B2 expression cassette driven by the promoter and optimized in sequence is shown by SEQ ID NO.4. The promoter provided by the invention can drive genes to express efficiently in plant anthers. The rice gene OsCYP704B2 expression cassette provided by the invention can achieve gene OsCYP704B2 being driven by the promoter to express in the anthers in a high level and fully recovering rice male sterility caused by the lack of OsCYP704B2 functions, has high hereditary stability and can be usedfor recovering the male fertility of rice or preparing nucleic male sterility maintainer lines and male sterile lines of the rice.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to an expression cassette containing a wild rice spotted OpCYP704B2 gene promoter capable of restoring the male fertility of rice OsCYP704B2 mutants and its application. Background technique [0002] Rice is one of the most important food crops, and hybrid rice has played an important role in increasing food production. Hybrid rice is the selection of two rice parents with different genetic backgrounds and complementary traits to produce the first-generation hybrids with heterosis for rice production. Hybrid rice has obvious heterosis phenomenon, which can increase yield by 30% compared with conventional rice. At present, hybrid rice can be divided into three-line hybrid rice and two-line hybrid rice. Three-line hybrid rice (three-line hybrid rice) means that the production of this hybrid rice requires three rice lines: rice cytoplasmic male sterile line, rice cytoplasmic ma...

AI Technical Summary

Problems solved by technology

The three-line breeding method has problems such as disease susceptibility, poor quality, and low breeding efficiency, and due to the constraints of the restoration relationship, 95% of rice resources cannot be used for hybrid breeding

[0006] Finally, the larger the transformed fragment, the lower the transformation efficiency

Therefore, Li et al. used the rice original genome sequence up to 3.8 kb (excluding the flanking vector sequence) as the restoration gene fragment, which has a high possibility of genetic instability in engineering production applications, and also increases vector construction and Difficulty of transformation; also difficult to identify the genotype of the mutation site

In summary, the recovery DNA fragments and vectors developed by Li et al. are not suitable for engineering seed breeding techniques, and there is an urgent need to develop recovery DNA fragments and vectors with higher genetic stability, easier construction and transformation, and convenient detection of mutant genotypes

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