Method for artificially creating plants male sterility

A male sterility and plant technology, applied in botany equipment and methods, plant products, plant gene improvement, etc., can solve problems such as interfering with anthers, antisense fragments are difficult to reach the action site, and abortion traits cannot be stably inherited
CN101260409AInactive Publication Date: 2008-09-10LIAOCHENG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LIAOCHENG UNIV
Publication Date
2008-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method of male sterility in plant by artificial hybridization, which is characterized in that: the method is a method which inhibits the expression of an endogenous HSP70 gene of plant anther through the antisense RNA technology, and interferes the normal growth of the transgenic plant anther so as to acquire a male sterility transgenic plant. In particular, the method is a method which transforms an antisense RNA expression vector of the HSP70 gene driven by an Osg6B promoter expressed by anther specificity into a graminaceous plant, inhibits the expression of the endogenous HSP70 gene of the anther, and interferes the normal growth of the transgenic plant anther so as to acquire the male sterility transgenic plant. The transgenic plant acquired by the method can implement male complete sterility.
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Description

technical field

[0001] The invention relates to a method for artificially creating male sterility of plants. Specifically, it relates to an antisense RNA expression vector of HSP70 gene driven by anther-specifically expressed Osg6B promoter, which is transferred into Gramineae grain crops to suppress the expression of endogenous HSP70 gene, thereby interfering with the anther growth of transgenic plants. A method for achieving male sterility through normal development, which can lay the foundation for the utilization of heterosis in plants. Background technique

[0002] Plant male sterility is the basis of plant heterosis utilization. Usually, the way to obtain plant male sterility is to screen out male sterile mutants in natural resources or through mutagenesis, or through distantly related species. These two methods have disadvantages such as difficulty in obtaining sterile materials and long cycle time. With the development of plant molecular biology and developmental b...

Claims

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