Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for artificially creating plants male sterility

A male sterility and plant technology, applied in botany equipment and methods, plant products, plant gene improvement, etc., can solve problems such as interfering with anthers, antisense fragments are difficult to reach the action site, and abortion traits cannot be stably inherited

Inactive Publication Date: 2008-09-10
LIAOCHENG UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method is still not completely free from the influence of the Mariani method
[0005] The third method is to use antisense RNA technology or other related technologies to interfere with the normal gene expression during anther (pollen) development and lead to male sterility
However, due to the method of in vitro injection, this method has the disadvantages that the antisense fragment is difficult to reach the action site and the abortion traits cannot be stably inherited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for artificially creating plants male sterility
  • Method for artificially creating plants male sterility
  • Method for artificially creating plants male sterility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] 1. Construction of expression vector

[0031] 1.1 Cloning of rice anther-specific promoter Osg6B

[0032] According to the Osg6B sequence reported in the literature (Tsuchiya and Toriyama et al., 1994) (the sequence shown in SEQ ID NO: 1, NCBI accession number: D21160), the primers were designed using DNASTAR software and synthesized and amplified by Beijing Saibaisheng Biotechnology Company Primers: 6B F-EcoRI 5'ggg gaa ttc ttt ttt tta cac ag3' (SEQ ID NO:2), 6B R-NcoI 5'cca tgg tgg att aga gct tg-3' (SEQ ID NO:3). The genomic DNA of the japonica rice variety Nipponbare was used as a PCR amplification template for PCR amplification. The amplification program is: 94°C 5min; 94°C 45sec, 56°C 45sec, 72°C 2min, 35r; 72°C 5min. (Taq enzyme used in PCR was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd., and dNTPs were purchased from Promega Company). After the amplification was completed, the agarose gel (agarose was purchased from Beijing Baierdi Biote...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method of male sterility in plant by artificial hybridization, which is characterized in that: the method is a method which inhibits the expression of an endogenous HSP70 gene of plant anther through the antisense RNA technology, and interferes the normal growth of the transgenic plant anther so as to acquire a male sterility transgenic plant. In particular, the method is a method which transforms an antisense RNA expression vector of the HSP70 gene driven by an Osg6B promoter expressed by anther specificity into a graminaceous plant, inhibits the expression of the endogenous HSP70 gene of the anther, and interferes the normal growth of the transgenic plant anther so as to acquire the male sterility transgenic plant. The transgenic plant acquired by the method can implement male complete sterility.

Description

technical field [0001] The invention relates to a method for artificially creating male sterility of plants. Specifically, it relates to an antisense RNA expression vector of HSP70 gene driven by anther-specifically expressed Osg6B promoter, which is transferred into Gramineae grain crops to suppress the expression of endogenous HSP70 gene, thereby interfering with the anther growth of transgenic plants. A method for achieving male sterility through normal development, which can lay the foundation for the utilization of heterosis in plants. Background technique [0002] Plant male sterility is the basis of plant heterosis utilization. Usually, the way to obtain plant male sterility is to screen out male sterile mutants in natural resources or through mutagenesis, or through distantly related species. These two methods have disadvantages such as difficulty in obtaining sterile materials and long cycle time. With the development of plant molecular biology and developmental b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/82C12N15/11A01H1/00A01H5/00
Inventor 刘立科刘春光刘根齐侯宁张文会陈建南
Owner LIAOCHENG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com