Specific promoter GmFTL2 for anthers, ovules and root caps of soybeans and application of specific promoter GmFTL2

A specific promoter, promoter technology, applied in the fields of genetic engineering and molecular biology
CN106906216AActive Publication Date: 2017-06-30INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2017-06-30

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Abstract

The invention discloses a specific promoter GmFTL2 for anthers, ovules and root caps of soybeans. The nucleotide sequence of the specific promoter GmFTL2 is shown as SEQ NO:1. The invention further provides an application of the promoter in regulation of downstream gene expression. The promoter GmFTL2 is used for overexpressing a GUS gene in arabidopsis thaliana, and results show that the promoter GmFTL2 can obviously increase expression quantity of the GUS gene in anthers, ovules and root caps of plants, so that the promoter GmFTL2 has specificity in the anthers, the ovules and the root caps of the plants and is suitable for spherically expressing a target gene in the anthers, the ovules and the root caps of the plants such as various crops, forests, vegetables, flowers, grass and the like.
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Description

technical field

[0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and in particular relates to a soybean anther, ovule and root cap specific promoter GmFTL2 and its application. Background technique

[0002] The promoter is an important cis-acting element in a gene, generally located tens of bases upstream of the transcription initiation site. According to different transcription modes, promoters can be divided into three types: constitutive promoters, tissue or organ-specific promoters, and inducible promoters. The activity of tissue- or organ-specific promoters is induced and regulated by specific tissue cell structures and chemical and physical signals, that is, the expression of such promoters has specific spatiotemporal characteristics. Driven by such promoters, the expression of genes is often limited to certain specific organs or tissue parts or specific developmental periods. Tissue or organ-specific promoters can no...

Claims

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