Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof

A related protein and plant flower technology, applied in the field of regulation and control of plant anther dehiscence-related protein TaAOC and its gene and application, can solve the problems of wheat and other problems

Active Publication Date: 2012-05-02
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof

Examples

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Embodiment 1

[0067] Example 1: cDNA cloning and semi-quantitative analysis of TaAOC, a gene related to the regulation of anther dehiscence in the jasmonic acid metabolic pathway of wheat

[0068]The anthers of the hybrid wheat variety Jingmai 7 (Jingshenmai 2009003) were taken, and the total RNA of the anthers was extracted by Trizol method. 5'RACE kit (5'RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3'RACE kit (3'RACE System for Rapid Amplification of cDNA Ends Kit) ( GIBCOBRL, CAT. NO. 18373-019) obtained the TaAOC gene, the full length of which is 845kb.

[0069] Total RNA from anthers of hybrid wheat variety Jingmai 7 (Jingshenmai 2009003) was extracted by Trizol method, and cDNA was obtained by reverse transcription with superscript II (purchased from Invitrogen) reverse transcriptase. Primers P1 and P2 were designed according to the sequence of TaAOC gene coding region. Using the cDNA obtained by reverse transcription as a template, PCR ampl...

Embodiment 2

[0075] Example 2: Construction of TaAOC monocot transgenic expression vector

[0076] 1. Construction of recombinant expression vector

[0077] Construction of pAHC-TaAOC monocotyledonous recombinant expression vector: The cDNA obtained by reverse transcription of the total RNA of the hybrid wheat variety Jingmai 7 (Jingshenmai 2009003) was used as a template, and PCR was carried out with specific primers containing BamHI and SacI linker sequences Amplification; then BamHI and SacI double digestion PCR product, recovery, the digestion product is inserted into the vector pAHC25 CaMV 29A promoter between the BamHI and SacI digestion sites, to obtain the recombinant vector pAHC-TaAOC.

[0078] The primer sequences are as follows:

[0079] AOC[BamHI]: 5′-CGGGATCTATCATCCGTTAGC-3′

[0080] AOC[SacI]: 5′-CGAGCTCACGCACGCAAGTTC-3′

[0081] 2. Acquisition and identification of transgenic wheat

[0082] 1) The recombinant plasmid pAHC-TaAOC obtained above is mediated by the gene gun ...

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Abstract

The invention relates to the field of genetic engineering, in particular to a relevant protein TaAOC for regulating and controlling the cracking of a plant anther as well as a gene and application thereof. The invention provides the relevant protein TaAOC for regulating and controlling the cracking of the plant anther. The amino acid sequence of the relevant protein TaAOC is shown as SEQ ID NO.1.In addition, the invention also provides the gene for coding the relevant protein for regulating and controlling the cracking of the plant anther. The amino acid sequence of the gene is shown as SEQ ID NO.2 or SEQ ID NO.3. Furthermore, the invention provides a recombinant vector containing the gene and application of the relevant protein TaAOC. The gene provided by the invention is correlated with the fertility of the plants, can be used for improving the cracking rate and the setting percentage of the plant anther and has very important theoretical and actual significances for increasing theyield of grains and developing the two-line hybrid wheat theory.

Description

technical field [0001] The invention relates to the field of genetic engineering, and in particular, the invention relates to TaAOC, a protein related to regulating plant anther dehiscence, and its gene and application. Background technique [0002] In modern agricultural production, people widely use heterosis to improve varieties. Heterosis refers to the phenomenon that the hybrid generation produced by the crossing of two parents with different genetic composition is superior to its parents in terms of viability, growth vigor, fecundity, stress resistance, yield and quality. The utilization of heterosis is considered to be the main way to greatly improve the wheat production level in the future. [0003] Male sterility is a common phenomenon in the plant kingdom. Male sterility refers to the sterility of plants due to the inability to produce functional pollen sacs, pollen or male gametes. Its main feature is the abnormal development of stamens, including two situation...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/53C12N15/63A01H5/00
Inventor 张立平刘泽涛马骅向群赵昌平单福华张风廷唐益苗
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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