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Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof

A related protein and plant flower technology, applied in the field of regulation and control of plant anther dehiscence-related protein TaAOC and its gene and application, can solve the problems of wheat and other problems

Active Publication Date: 2013-04-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on AOC in wheat

Method used

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  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof
  • Relevant protein TaAOC for regulating and controlling cracking of plant anther as well as gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: cDNA cloning and semi-quantitative analysis of anther dehiscence-related gene TaAOC in the wheat jasmonic acid metabolic pathway

[0068]The anthers of the hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) were taken, and the total RNA of the anthers was extracted by Trizol method. 5'RACE kit (5'RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3'RACE kit (3'RACE System for Rapid Amplification of cDNA Ends Kit) ( GIBCOBRL, CAT.NO.18373-019) obtained the TaAOC gene, the full length of which is 845kb.

[0069] The total RNA of the anthers of hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) was extracted by Trizol method, and cDNA was obtained by reverse transcription with superscript II (purchased from Invitrogen Company) reverse transcriptase. Primers P1 and P2 were designed according to the sequence of TaAOC gene coding region. Using the cDNA obtained by reverse transcription as a template, PCR amplificati...

Embodiment 2

[0075] Embodiment 2: Construction of TaAOC monocot transgene expression vector

[0076] 1. Construction of recombinant expression vector

[0077] Construction of pAHC-TaAOC monocot recombinant expression vector: using the cDNA obtained by reverse transcription of the total RNA of the hybrid wheat variety Jingmai No. 7 (Jingshenmai 2009003) as a template, PCR was carried out with specific primers containing BamHI and SacI linker sequences Amplify; then BamHI and SacI double-digest the PCR product, recover, and insert the digested product between the BamHI and SacI restriction sites behind the CaMV 29A promoter of the vector pAHC25 in the forward direction to obtain the recombinant vector pAHC-TaAOC.

[0078] The primer sequences are as follows:

[0079] AOC[BamHI]: 5′-CGGGATCCTATCATCCGTTAGC-3′

[0080] AOC[SacI]: 5′-CGAGCTCACGCACGCAAGTTC-3′

[0081] 2. Acquisition and Identification of Transgenic Wheat

[0082] 1) The recombinant plasmid pAHC-TaAOC obtained above was mediat...

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Abstract

The invention relates to the field of genetic engineering, in particular to a relevant protein TaAOC for regulating and controlling the cracking of a plant anther as well as a gene and application thereof. The invention provides the relevant protein TaAOC for regulating and controlling the cracking of the plant anther. The amino acid sequence of the relevant protein TaAOC is shown as SEQ ID NO.1.In addition, the invention also provides the gene for coding the relevant protein for regulating and controlling the cracking of the plant anther. The amino acid sequence of the gene is shown as SEQ ID NO.2 or SEQ ID NO.3. Furthermore, the invention provides a recombinant vector containing the gene and application of the relevant protein TaAOC. The gene provided by the invention is correlated with the fertility of the plants, can be used for improving the cracking rate and the setting percentage of the plant anther and has very important theoretical and actual significances for increasing theyield of grains and developing the two-line hybrid wheat theory.

Description

technical field [0001] The present invention relates to the field of genetic engineering, in particular, the present invention relates to a protein TaAOC related to regulation and control of plant anther dehiscence, its gene and application. Background technique [0002] In modern agricultural production, people widely use heterosis to improve varieties. Heterosis refers to the phenomenon that the first generation of hybrids produced by the hybridization of two parents with different genetic compositions has advantages over their parents in vigor, growth potential, fecundity, stress resistance, yield and quality. Utilization of heterosis is considered to be the main way to greatly increase the wheat production level in the future. [0003] Male sterility is a ubiquitous phenomenon in the plant world. Male sterility refers to the sterility of plants caused by the inability to produce functional anthers, pollen or male gametes. Its main feature is abnormal development of st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/63A01H5/00
Inventor 张立平刘泽涛马骅向群赵昌平单福华张风廷唐益苗
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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